KCNJ1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KCNJ1_HUMAN
• UniProt AC: P48048
• Protein Name: ATP-sensitive inward rectifier potassium channel 1
• Gene Name: KCNJ1
• Organism: Homo sapiens (Human).
• Sequence Length: 391
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.
• Protein Description: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium..
• Protein Sequence: MNASSRNVFDTLIRVLTESMFKHLRKWVVTRFFGHSRQRARLVSKDGRCNIEFGNVEAQSRFIFFVDIWTTVLDLKWRYKMTIFITAFLGSWFFFGLLWYAVAYIHKDLPEFHPSANHTPCVENINGLTSAFLFSLETQVTIGYGFRCVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTVTPEGETIILDQININFVVDAGNENLFFISPLTIYHVIDHNSPFFHMAAETLLQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFAPIVSKTKEGKYRVDFHNFSKTVEVETPHCAMCLYNEKDVRARMKRGYDNPNFILSEVNETDDTKM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MNASSRNVFDT ----CCHHHHHHHHH		18547545
5	Phosphorylation	---MNASSRNVFDTL ---CCHHHHHHHHHH		29759185
11	Phosphorylation	SSRNVFDTLIRVLTE HHHHHHHHHHHHHHH		29759185
19	Phosphorylation	LIRVLTESMFKHLRK HHHHHHHHHHHHHHH		22210691
44	Phosphorylation	RQRARLVSKDGRCNI HHHEEEEECCCCEEE		18547545
117	N-linked_Glycosylation	PEFHPSANHTPCVEN CCCCCCCCCCCCCCC		10889209
164	Phosphorylation	IFLLIFQSILGVIIN HHHHHHHHHHHHHHH		8621594
183	Phosphorylation	GAILAKISRPKKRAK HHHHHHHCCCCCCCC		8621594
191	Phosphorylation	RPKKRAKTITFSKNA CCCCCCCEEEECCCE		18547545
193	Phosphorylation	KKRAKTITFSKNAVI CCCCCEEEECCCEEE		18547545
201	Phosphorylation	FSKNAVISKRGGKLC ECCCEEECCCCCEEE		18547545
202	Acetylation	SKNAVISKRGGKLCL CCCEEECCCCCEEEE		22424773
219	Phosphorylation	RVANLRKSLLIGSHI EHHCCCHHHHHCHHH		18620882
234	Phosphorylation	YGKLLKTTVTPEGET HHCCCCEEECCCCCE		18547545
313	Phosphorylation	ATCQVRTSYVPEEVL EEEEEECCCCCHHHE		18620882
337	Phosphorylation	SKTKEGKYRVDFHNF ECCCCCCEEEEEECC		12556363
381	Phosphorylation	DNPNFILSEVNETDD CCCCCEEEECCCCCC		28387310
389	Phosphorylation	EVNETDDTKM----- ECCCCCCCCC-----		28387310

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
4	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
44	S	Phosphorylation	Kinase	SGK1	O00141	Uniprot
44	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
44	S	Phosphorylation	Kinase	SGK-FAMILY	-	GPS
44	S	Phosphorylation	Kinase	SGK_GROUP	-	PhosphoELM
193	T	Phosphorylation	Kinase	PKCA	P17252	PSP
201	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
219	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
313	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
337	Y	Phosphorylation	Kinase	SRC	P12931	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	SH3RF1	Q7Z6J0	PMID:19710010

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
44	S	Phosphorylation	Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.	12684516

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KCNJ1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NHRF1_HUMAN	SLC9A3R1	physical	14604981
NHRF2_HUMAN	SLC9A3R2	physical	14604981
SH3R1_HUMAN	SH3RF1	physical	19710010

Drug and Disease Associations

• Kegg Disease
• H00239: Bartter syndrome
• OMIM Disease
• 241200: Bartter syndrome 2 (BS2)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00217: Bethanidine
• DB00222: Glimepiride
• DB01016: Glyburide
• DB01382: Glycodiazine
• DB00350: Minoxidil
• DB00839: Tolazamide
• DB01124: Tolbutamide
• DB01392: Yohimbine