KCMA1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KCMA1_RAT
• UniProt AC: Q62976
• Protein Name: Calcium-activated potassium channel subunit alpha-1
• Gene Name: Kcnma1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1209
• Subcellular Localization: Cell membrane; Multi-pass membrane protein.; Isoform 7: Endoplasmic reticulum membrane; Multi-pass membrane protein. Has a dominant-negative effect on other isoforms, preventing their localization to the cell membrane.
• Protein Description: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX)..
• Protein Sequence: MANGGGGGGGGSSGSSGGGGGGGGGETALRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELAKPGKLPLVSVNQEKNSGTHILMITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	GGGGGGGSSGSSGGG CCCCCCCCCCCCCCC	34.50	18573811
27	Phosphorylation	GGGGGGETALRMSSN CCCCCHHHHCHHCCC	33.98	18573811
119	S-palmitoylation	LKYLWTVCCHCGGKT HHHHHHHHHHCCCCC	0.74	-
120	S-palmitoylation	KYLWTVCCHCGGKTK HHHHHHHHHCCCCCC	2.30	-
122	S-palmitoylation	LWTVCCHCGGKTKEA HHHHHHHCCCCCCCC	4.85	-
136	Phosphorylation	AQKINNGSSQADGTL CHHCCCCCCCCCCCC	22.77	22673903
137	Phosphorylation	QKINNGSSQADGTLK HHCCCCCCCCCCCCC	30.68	22673903
142	Phosphorylation	GSSQADGTLKPVDEK CCCCCCCCCCCCCCC	31.96	22673903
519	Acetylation	SIKNYHPKIRIITQM HCCCCCHHHHHHHHH	31.16	22902405
581	Phosphorylation	ANLFSMRSFIKIEED HHHHHCHHCEECCCC	23.76	18573811
584	Ubiquitination	FSMRSFIKIEEDTWQ HHCHHCEECCCCCHH	41.89	-
710	Phosphorylation	LEDEQPPTLSPKKKQ CCCCCCCCCCCCCCC	46.69	28551015
712	Phosphorylation	DEQPPTLSPKKKQRN CCCCCCCCCCCCCCC	36.83	27097102
725	Phosphorylation	RNGGMRNSPNTSPKL CCCCCCCCCCCCCHH	14.79	18573811
728	Phosphorylation	GMRNSPNTSPKLMRH CCCCCCCCCCHHHCC	50.19	25403869
729	Phosphorylation	MRNSPNTSPKLMRHD CCCCCCCCCHHHCCC	27.03	25403869
742 (in isoform 8)	Phosphorylation	-	43.27	25403869
773 (in isoform 8)	Phosphorylation	-	2.55	30240740
805	Dimethylation	SSALIGLRNLVMPLR HHHHHHHHHHHHCCH	29.26	-
805	Methylation	SSALIGLRNLVMPLR HHHHHHHHHHHHCCH	29.26	4199105
847	Phosphorylation	LHNFPKVSILPGTPL HHCCCCEEECCCCCC	25.24	18573811
913	Phosphorylation	IGVLQANSQGFTPPG CCEEECCCCCCCCCC	34.39	25403869
917	Phosphorylation	QANSQGFTPPGMDRS ECCCCCCCCCCCCCC	35.08	25403869
924	Phosphorylation	TPPGMDRSSPDNSPV CCCCCCCCCCCCCCC	41.79	25403869
925	Phosphorylation	PPGMDRSSPDNSPVH CCCCCCCCCCCCCCC	36.85	30240740
929	Phosphorylation	DRSSPDNSPVHGMLR CCCCCCCCCCCCCCC	35.36	30240740
939	Phosphorylation	HGMLRQPSITTGVNI CCCCCCCCCCCCCCC	24.99	18573811
962	Phosphorylation	PGKLPLVSVNQEKNS CCCCCEEEECCCCCC	24.04	18573811
1061	Phosphorylation	NALRGGYSTPQTLAN HHHCCCCCCHHHHCC	36.56	22673903
1062	Phosphorylation	ALRGGYSTPQTLANR HHCCCCCCHHHHCCH	15.78	25403869
1065	Phosphorylation	GGYSTPQTLANRDRC CCCCCHHHHCCHHHC	29.10	25403869
1120	Phosphorylation	RLRDAHLSTPSQCTK EEHHCCCCCCCCCCE	28.53	18573811
1162	Phosphorylation	NAGQSRASLSHSSHS CCCCCCHHHCCCCCC	29.33	18573811
1164	Phosphorylation	GQSRASLSHSSHSSQ CCCCHHHCCCCCCCC	20.87	27115346
1167	Phosphorylation	RASLSHSSHSSQSSS CHHHCCCCCCCCCCC	23.20	18573811
1173	Phosphorylation	SSHSSQSSSKKSSSV CCCCCCCCCCCCCCC	38.24	18573811
1174	Phosphorylation	SHSSQSSSKKSSSVH CCCCCCCCCCCCCCC	49.72	18573811
1177	Phosphorylation	SQSSSKKSSSVHSIP CCCCCCCCCCCCCCC	31.42	25575281
1178	Phosphorylation	QSSSKKSSSVHSIPS CCCCCCCCCCCCCCC	45.30	25575281
1179	Phosphorylation	SSSKKSSSVHSIPST CCCCCCCCCCCCCCC	30.82	25575281
1182	Phosphorylation	KKSSSVHSIPSTANR CCCCCCCCCCCCCCC	33.76	30240740
1185	Phosphorylation	SSVHSIPSTANRPNR CCCCCCCCCCCCCCC	38.18	25575281
1186	Phosphorylation	SVHSIPSTANRPNRP CCCCCCCCCCCCCCC	23.69	22668510
1195	Phosphorylation	NRPNRPKSRESRDKQ CCCCCCCCCCCHHHH	43.92	-
1198	Phosphorylation	NRPKSRESRDKQKKE CCCCCCCCHHHHHHH	44.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KCMA1_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KCMA1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KCMA1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.