dbPTM

K2C8_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	K2C8_RAT
UniProt AC	Q10758
Protein Name	Keratin, type II cytoskeletal 8
Gene Name	Krt8
Organism	Rattus norvegicus (Rat).
Sequence Length	483
Subcellular Localization	Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Protein Description	Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle..
Protein Sequence	MSVRVTQKSYKMSTSGPRAFSSRSFTSGPGARISSSSFSRVGSSSSSFRGSLGGFGGAGVGGITAVTVNQSLLNPLKLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTSRSNMDNMFESYINNLRRQLEALGQEKLKLEVELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQIHEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVRAQYEEIANRSRAEAETMYQIKYEELQTLAGKHGDDLRRSKTEISEMNRNISRLQAEIDALKGQRATLEAAIADAEQRGELAVKDANAKLEDLKNALQKAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSSYGGLTSPGFSYGMSSFQPGFGSVGGSSTYSRTKAVVVKKIETRDGKLVSESSDIMSK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSVRVTQKS ------CCCEEEECC	20.73	22673903
9	Phosphorylation	SVRVTQKSYKMSTSG CCEEEECCEECCCCC	21.84	22817900
10	Phosphorylation	VRVTQKSYKMSTSGP CEEEECCEECCCCCC	20.11	23984901
13	Phosphorylation	TQKSYKMSTSGPRAF EECCEECCCCCCCCC	18.44	22817900
14	Phosphorylation	QKSYKMSTSGPRAFS ECCEECCCCCCCCCC	33.92	23984901
15	Phosphorylation	KSYKMSTSGPRAFSS CCEECCCCCCCCCCC	39.22	23984901
21	Phosphorylation	TSGPRAFSSRSFTSG CCCCCCCCCCCCCCC	24.69	29779826
22	Phosphorylation	SGPRAFSSRSFTSGP CCCCCCCCCCCCCCC	26.03	16641100
23	Methylation	GPRAFSSRSFTSGPG CCCCCCCCCCCCCCC	34.29	-
24	Phosphorylation	PRAFSSRSFTSGPGA CCCCCCCCCCCCCCC	34.52	23712012
26	Phosphorylation	AFSSRSFTSGPGARI CCCCCCCCCCCCCEE	33.74	16641100
27	Phosphorylation	FSSRSFTSGPGARIS CCCCCCCCCCCCEEC	40.77	16641100
32	Methylation	FTSGPGARISSSSFS CCCCCCCEECCCCCC	34.93	-
34	Phosphorylation	SGPGARISSSSFSRV CCCCCEECCCCCCCC	20.72	22817900
35	Phosphorylation	GPGARISSSSFSRVG CCCCEECCCCCCCCC	27.85	29779826
36	Phosphorylation	PGARISSSSFSRVGS CCCEECCCCCCCCCC	28.72	23984901
37	Phosphorylation	GARISSSSFSRVGSS CCEECCCCCCCCCCC	29.23	16396499
39	Phosphorylation	RISSSSFSRVGSSSS EECCCCCCCCCCCCC	28.18	22673903
40	Methylation	ISSSSFSRVGSSSSS ECCCCCCCCCCCCCC	34.50	-
43	Phosphorylation	SSFSRVGSSSSSFRG CCCCCCCCCCCCCCC	24.96	23712012
44	Phosphorylation	SFSRVGSSSSSFRGS CCCCCCCCCCCCCCC	28.45	16396499
45	Phosphorylation	FSRVGSSSSSFRGSL CCCCCCCCCCCCCCC	31.53	16396499
46	Phosphorylation	SRVGSSSSSFRGSLG CCCCCCCCCCCCCCC	35.13	16396499
47	Phosphorylation	RVGSSSSSFRGSLGG CCCCCCCCCCCCCCC	22.40	23712012
49	Asymmetric dimethylarginine	GSSSSSFRGSLGGFG CCCCCCCCCCCCCCC	35.42	-
49	Methylation	GSSSSSFRGSLGGFG CCCCCCCCCCCCCCC	35.42	-
51	Phosphorylation	SSSSFRGSLGGFGGA CCCCCCCCCCCCCCC	21.42	16641100
64	Phosphorylation	GAGVGGITAVTVNQS CCCCCCEEEEEECHH	20.53	23984901
67	Phosphorylation	VGGITAVTVNQSLLN CCCEEEEEECHHHCC	15.98	23984901
71	Phosphorylation	TAVTVNQSLLNPLKL EEEEECHHHCCCCCC	29.62	23984901
89	Phosphorylation	PNIQAVRTQEKEQIK CCCHHHHCCCHHHHH	34.19	23984901
97	Phosphorylation	QEKEQIKTLNNKFAS CCHHHHHHHHHHHHH	36.03	23984901
101	Acetylation	QIKTLNNKFASFIDK HHHHHHHHHHHHHHH	41.03	22902405
101	Malonylation	QIKTLNNKFASFIDK HHHHHHHHHHHHHHH	41.03	-
101	N6-malonyllysine	QIKTLNNKFASFIDK HHHHHHHHHHHHHHH	41.03	-
104	Phosphorylation	TLNNKFASFIDKVRF HHHHHHHHHHHHHHH	26.35	22673903
108	Acetylation	KFASFIDKVRFLEQQ HHHHHHHHHHHHHHH	30.41	22902405
117	Acetylation	RFLEQQNKMLETKWS HHHHHHHHHHHHHHH	40.29	22368959
122	Acetylation	QNKMLETKWSLLQQQ HHHHHHHHHHHHHHH	25.96	22902405
124	Phosphorylation	KMLETKWSLLQQQKT HHHHHHHHHHHHHHH	22.28	23984901
134	Phosphorylation	QQQKTSRSNMDNMFE HHHHHCCCCHHHHHH	35.95	28689409
142	Phosphorylation	NMDNMFESYINNLRR CHHHHHHHHHHHHHH	21.76	28689409
158	Acetylation	LEALGQEKLKLEVEL HHHHCCCCHHHEEEH	42.93	22902405
178	Acetylation	LVEDFKNKYEDEINK HHHHHHHHHHHHHHH	51.08	15606717
198	Acetylation	NEFVLIKKDVDEAYM CEEEEEECCCCHHHH	56.86	22902405
204	Phosphorylation	KKDVDEAYMNKVELE ECCCCHHHHCHHHHH	10.12	25575281
207	Acetylation	VDEAYMNKVELESRL CCHHHHCHHHHHHHH	22.13	22902405
237	Phosphorylation	EEIRELQSQISDTSV HHHHHHHHHHCCCEE	41.95	23984901
240	Phosphorylation	RELQSQISDTSVVLS HHHHHHHCCCEEEEE	27.48	23984901
242	Phosphorylation	LQSQISDTSVVLSMD HHHHHCCCEEEEEEC	19.34	23984901
243	Phosphorylation	QSQISDTSVVLSMDN HHHHCCCEEEEEECC	18.41	21630457
247	Phosphorylation	SDTSVVLSMDNSRSL CCCEEEEEECCCCCC	16.69	23984901
253	Phosphorylation	LSMDNSRSLDMDSII EEECCCCCCCHHHHH	28.65	22108457
258	Phosphorylation	SRSLDMDSIIAEVRA CCCCCHHHHHHHHHH	14.84	21630457
267	Phosphorylation	IAEVRAQYEEIANRS HHHHHHHHHHHHHHH	17.97	25575281
274	Phosphorylation	YEEIANRSRAEAETM HHHHHHHHHHHHHHH	35.33	29779826
280	Phosphorylation	RSRAEAETMYQIKYE HHHHHHHHHHHHCHH	28.38	23984901
282	Phosphorylation	RAEAETMYQIKYEEL HHHHHHHHHHCHHHH	17.95	23984901
285	Acetylation	AETMYQIKYEELQTL HHHHHHHCHHHHHHH	30.96	22902405
291	Phosphorylation	IKYEELQTLAGKHGD HCHHHHHHHCHHCCH	31.06	23984901
295	Acetylation	ELQTLAGKHGDDLRR HHHHHCHHCCHHHHH	38.52	22902405
303	Phosphorylation	HGDDLRRSKTEISEM CCHHHHHCHHHHHHH	37.18	22668510
304	Succinylation	GDDLRRSKTEISEMN CHHHHHCHHHHHHHH	48.65	26843850
304	Acetylation	GDDLRRSKTEISEMN CHHHHHCHHHHHHHH	48.65	22902405
305	Phosphorylation	DDLRRSKTEISEMNR HHHHHCHHHHHHHHH	39.54	22668510
308	Phosphorylation	RRSKTEISEMNRNIS HHCHHHHHHHHHHHH	25.26	28689409
315	Phosphorylation	SEMNRNISRLQAEID HHHHHHHHHHHHHHH	30.41	23984901
325	Acetylation	QAEIDALKGQRATLE HHHHHHHHHHHHHHH	55.51	22902405
330	Phosphorylation	ALKGQRATLEAAIAD HHHHHHHHHHHHHHC	27.59	23984901
347	Acetylation	QRGELAVKDANAKLE HHCCHHHHHHHHHHH	45.58	22902405
352	Acetylation	AVKDANAKLEDLKNA HHHHHHHHHHHHHHH	52.82	22902405
357	Acetylation	NAKLEDLKNALQKAK HHHHHHHHHHHHHHH	53.12	22902405
393	Acetylation	IEIATYRKLLEGEES HHHHHHHHHHCCCHH	47.19	3967001
400	Phosphorylation	KLLEGEESRLESGMQ HHHCCCHHHHHHHCC	37.55	23984901
404	Phosphorylation	GEESRLESGMQNMSI CCHHHHHHHCCCCEE	44.12	23984901
410	Phosphorylation	ESGMQNMSIHTKTTS HHHCCCCEEEECCCC	20.83	28689409
413	Phosphorylation	MQNMSIHTKTTSGYA CCCCEEEECCCCCCC	28.54	28689409
415	Phosphorylation	NMSIHTKTTSGYAGG CCEEEECCCCCCCCC	27.61	22673903
416	Phosphorylation	MSIHTKTTSGYAGGL CEEEECCCCCCCCCC	23.01	22673903
417	Phosphorylation	SIHTKTTSGYAGGLS EEEECCCCCCCCCCC	34.73	22817900
419	Phosphorylation	HTKTTSGYAGGLSSS EECCCCCCCCCCCCC	11.22	22673903
424	Phosphorylation	SGYAGGLSSSYGGLT CCCCCCCCCCCCCCC	22.10	22673903
425	Phosphorylation	GYAGGLSSSYGGLTS CCCCCCCCCCCCCCC	32.40	22673903
426	Phosphorylation	YAGGLSSSYGGLTSP CCCCCCCCCCCCCCC	25.15	22817900
427	Phosphorylation	AGGLSSSYGGLTSPG CCCCCCCCCCCCCCC	19.70	22673903
431	Phosphorylation	SSSYGGLTSPGFSYG CCCCCCCCCCCCCCC	35.83	21630457
432	Phosphorylation	SSYGGLTSPGFSYGM CCCCCCCCCCCCCCC	28.24	22108457
436	Phosphorylation	GLTSPGFSYGMSSFQ CCCCCCCCCCCCCCC	27.05	22673903
437	Phosphorylation	LTSPGFSYGMSSFQP CCCCCCCCCCCCCCC	18.00	22673903
440	Phosphorylation	PGFSYGMSSFQPGFG CCCCCCCCCCCCCCC	24.52	22673903
441	Phosphorylation	GFSYGMSSFQPGFGS CCCCCCCCCCCCCCC	21.00	22673903
448	Phosphorylation	SFQPGFGSVGGSSTY CCCCCCCCCCCCCCC	18.48	22673903
464	Acetylation	RTKAVVVKKIETRDG CCEEEEEEEEEECCC	36.10	22902405
472	Acetylation	KIETRDGKLVSESSD EEEECCCCEECCCHH	50.43	22902405
475	Phosphorylation	TRDGKLVSESSDIMS ECCCCEECCCHHCCC	42.04	23712012
477	Phosphorylation	DGKLVSESSDIMSK- CCCEECCCHHCCCC-	26.29	22108457
478	Phosphorylation	GKLVSESSDIMSK-- CCEECCCHHCCCC--	27.40	29779826
482	Phosphorylation	SESSDIMSK------ CCCHHCCCC------	35.23	22108457

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
9	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
9	S	Phosphorylation	Kinase	PRKCE	P09216	Uniprot
13	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
24	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
24	S	Phosphorylation	Kinase	PRKCE	P09216	Uniprot
34	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
37	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
43	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
51	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
417	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
426	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of K2C8_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of K2C8_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of K2C8_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of K2C8_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomics of vasopressin-sensitive renal cells:regulation of aquaporin-2 phosphorylation at two sites."; Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.; Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-24; THR-26;SER-27; SER-475 AND SER-478, AND MASS SPECTROMETRY.	16641100 [Abstract]
"Keratin 8 phosphorylation in vitro by cAMP-dependent protein kinaseoccurs within the amino- and carboxyl-terminal end domains."; Ando S., Tokui T., Yano T., Inagaki M.; Biochem. Biophys. Res. Commun. 221:67-71(1996). Cited for: PROTEIN SEQUENCE OF 9-18; 24-75 AND 415-452, AND PHOSPHORYLATION ATSER-9; SER-13; SER-24; SER-34; SER-37; SER-43; SER-51; SER-417;SER-424 AND SER-426.	8660345 [Abstract]