JUN_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: JUN_RAT
• UniProt AC: P17325
• Protein Name: Transcription factor AP-1
• Gene Name: Jun
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 334
• Subcellular Localization: Nucleus .
• Protein Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells..
• Protein Sequence: MTAKMETTFYDDALNASFLQSESGAYGYSNPKILKQSMTLNLADPVGNLKPHLRAKNSDLLTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVSGAGMVAPAVASVAGAGGGGGYSASLHSEPPVYANLSNFNPGALSSGGGAPSYGATGLAFPSQPQQQQQPPQPPHHLPQQIPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTAKMETTF ------CCCCCCCEE	34.68	-
8	Phosphorylation	MTAKMETTFYDDALN CCCCCCCEEHHHHHC	13.59	26022182
26	Phosphorylation	LQSESGAYGYSNPKI HCCCCCCCCCCCHHH	21.81	26022182
28	Phosphorylation	SESGAYGYSNPKILK CCCCCCCCCCHHHHH	7.81	26022182
56	Acetylation	LKPHLRAKNSDLLTS CCHHHHHCCCCCCCC	51.02	-
58	Phosphorylation	PHLRAKNSDLLTSPD HHHHHCCCCCCCCCC	29.00	28689409
62	Phosphorylation	AKNSDLLTSPDVGLL HCCCCCCCCCCCCHH	45.57	27097102
63	Phosphorylation	KNSDLLTSPDVGLLK CCCCCCCCCCCCHHH	21.05	16831194
73	Phosphorylation	VGLLKLASPELERLI CCHHHHCCHHHHHEE	29.37	1749429
83	Phosphorylation	LERLIIQSSNGHITT HHHEEEECCCCCEEC	18.50	28689409
84	Phosphorylation	ERLIIQSSNGHITTT HHEEEECCCCCEECC	30.38	28689409
89	Phosphorylation	QSSNGHITTTPTPTQ ECCCCCEECCCCCCE	20.95	28689409
90	Phosphorylation	SSNGHITTTPTPTQF CCCCCEECCCCCCEE	30.12	28689409
91	Phosphorylation	SNGHITTTPTPTQFL CCCCEECCCCCCEEE	19.26	28689409
93	Phosphorylation	GHITTTPTPTQFLCP CCEECCCCCCEEECC	36.55	28689409
95	Phosphorylation	ITTTPTPTQFLCPKN EECCCCCCEEECCCC	34.14	28689409
234	Phosphorylation	ALKEEPQTVPEMPGE HHHHCCCCCCCCCCC	49.06	28689409
242	Phosphorylation	VPEMPGETPPLSPID CCCCCCCCCCCCCCC	35.80	21630457
246	Phosphorylation	PGETPPLSPIDMESQ CCCCCCCCCCCHHHH	26.11	1651323
252	Phosphorylation	LSPIDMESQERIKAE CCCCCHHHHHHHHHH	30.61	22668510
274	Acetylation	IAASKCRKRKLERIA HHHHHHHHHHHHHHH	64.45	-
289	Phosphorylation	RLEEKVKTLKAQNSE HHHHHHHHHHHHCHH	36.16	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2	T	Phosphorylation	Kinase	PAK2	Q64303	Uniprot
8	T	Phosphorylation	Kinase	PAK2	Q64303	Uniprot
63	S	Phosphorylation	Kinase	CDK1	P06493	PSP
63	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
63	S	Phosphorylation	Kinase	PLK3	Q9R011	Uniprot
63	S	Phosphorylation	Kinase	MAPK8	P49185	Uniprot
73	S	Phosphorylation	Kinase	CDK1	P06493	PSP
73	S	Phosphorylation	Kinase	PLK3	Q9R011	Uniprot
73	S	Phosphorylation	Kinase	JNK1	P49185	PSP
73	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
89	T	Phosphorylation	Kinase	PAK2	Q64303	Uniprot
93	T	Phosphorylation	Kinase	PAK2	Q64303	Uniprot
242	T	Phosphorylation	Kinase	GSK3-BETA	P18266	Uniprot
246	S	Phosphorylation	Kinase	MAPK3	P21708	GPS
246	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
246	S	Phosphorylation	Kinase	GSK3-BETA	P18266	Uniprot
246	S	Phosphorylation	Kinase	CDK1	P06493	PSP
246	S	Phosphorylation	Kinase	DYRK2	-	Uniprot
252	S	Phosphorylation	Kinase	GSK3-BETA	P18266	Uniprot
289	T	Phosphorylation	Kinase	PAK2	Q64303	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Map3k1	Q62925	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
242	T	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	-
242	T	Phosphorylation	Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242.	-
246	S	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	-
246	S	Phosphorylation	Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242.	-
252	S	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	-
271	K	Acetylation	Acetylated at Lys-271 by EP300.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of JUN_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.