ITSN1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ITSN1_RAT
• UniProt AC: Q9WVE9
• Protein Name: Intersectin-1
• Gene Name: Itsn1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1217
• Subcellular Localization: Endomembrane system. Cell junction, synapse, synaptosome. Cell projection, lamellipodium. Membrane, clathrin-coated pit. Colocalizes with SGIP1 at the plasma membrane in structures corresponding most probably to clathrin-coated pits..
• Protein Description: Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Inhibits ARHGAP31 activity toward RAC1..
• Protein Sequence: MAQFPTPFGGSLDIWAITVEERAKHDQQFQSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNKDGRMDQVEFSIAMKLIKLKLQGYQLPPALPPVMKQQPAAISSAPAFGIGGMAGMPPLTAVAPVPMGSIPVVGMSPPLVSSVPQAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASAPAAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSADQRLPEEPSSEDEQQVEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLTQRNKDQEGIVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILSDQLKQVQQNSLHRDSLLTLKRALEAKELARQQLREQLDEVEKETRSKLQEIDVFNNQLKELREIHSKQQLQKQRSIEAERLKQKEQERKSLELEKQKEEGQRRVQERDKQWQEHVQQEEQQRPRKPHEEDKLKREDSVKKKEAEERAKPEVQDKQSRLFHPHQEPAKPAQAPWPTTEKGPLTISAQESAKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGEPKGKTGWFPANYAEKIPENEIPTPAKPVTDLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSTNEKPETDNWDTWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTEAPSSLKRVASPAAKPAIPGEEFVAMYTYESSEHGDLTFQQGHVIVVTKKDGDWWTGTVGETSGVFPSNYVRLKDSEGSGTAGKTGSLEKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLSKEDPDWWKGEVSGQVGLFPSNYVKLTTDMDPSQQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
184	Phosphorylation	ATLPKSSSFSRSGPG CCCCCCCCCCCCCCC	34.04	16396499
188	Phosphorylation	KSSSFSRSGPGSQLN CCCCCCCCCCCCHHH	48.41	30181290
192	Phosphorylation	FSRSGPGSQLNTKLQ CCCCCCCCHHHHHHH	34.33	30181290
196	Phosphorylation	GPGSQLNTKLQKAQS CCCCHHHHHHHHHHC	41.55	30181290
203	Phosphorylation	TKLQKAQSFDVASAP HHHHHHHCCCCCCCC	28.42	29779826
208	Phosphorylation	AQSFDVASAPAAAEW HHCCCCCCCCHHHHH	34.33	27097102
313	Phosphorylation	PSFRRVRSGSGMSVI CCCCCCCCCCCCEEE	34.16	27097102
315	Phosphorylation	FRRVRSGSGMSVISS CCCCCCCCCCEEECC	32.35	23712012
318	Phosphorylation	VRSGSGMSVISSSSA CCCCCCCEEECCCCC	21.59	27097102
321	Phosphorylation	GSGMSVISSSSADQR CCCCEEECCCCCCCC	23.24	25403869
322	Phosphorylation	SGMSVISSSSADQRL CCCEEECCCCCCCCC	19.72	25403869
323	Phosphorylation	GMSVISSSSADQRLP CCEEECCCCCCCCCC	23.82	25403869
324	Phosphorylation	MSVISSSSADQRLPE CEEECCCCCCCCCCC	37.50	25403869
334	Phosphorylation	QRLPEEPSSEDEQQV CCCCCCCCCHHHHHH	50.03	23991683
335	Phosphorylation	RLPEEPSSEDEQQVE CCCCCCCCHHHHHHH	60.84	23991683
476	Phosphorylation	VLKARRKTLEFELEA EEEEECCEEHHHHHH	29.70	30181290
558	Phosphorylation	LKQVQQNSLHRDSLL HHHHHHCCCCHHHHH	22.63	29779826
563	Phosphorylation	QNSLHRDSLLTLKRA HCCCCHHHHHHHHHH	25.82	25575281
566	Phosphorylation	LHRDSLLTLKRALEA CCHHHHHHHHHHHHH	35.21	28432305
623	Phosphorylation	QQLQKQRSIEAERLK HHHHHHHHHHHHHHH	23.45	29779826
638	Phosphorylation	QKEQERKSLELEKQK HHHHHHHHHHHHHHH	33.04	29779826
685	Phosphorylation	DKLKREDSVKKKEAE HHHCHHHHHHHHHHH	30.96	29779826
888	Phosphorylation	AGQLRQRSAFTPATA CCCCCCCCCCCCCCC	21.28	28689409
889	Phosphorylation	GQLRQRSAFTPATAT CCCCCCCCCCCCCCC	17.96	-
891	Phosphorylation	LRQRSAFTPATATGS CCCCCCCCCCCCCCC	16.25	27097102
893	Phosphorylation	QRSAFTPATATGSSP CCCCCCCCCCCCCCC	13.55	-
894	Phosphorylation	RSAFTPATATGSSPS CCCCCCCCCCCCCCC	26.42	29779826
896	Phosphorylation	AFTPATATGSSPSPV CCCCCCCCCCCCCCC	32.99	27097102
898	Phosphorylation	TPATATGSSPSPVLG CCCCCCCCCCCCCCC	34.42	27097102
899	Phosphorylation	PATATGSSPSPVLGQ CCCCCCCCCCCCCCC	30.64	23712012
901	Phosphorylation	TATGSSPSPVLGQGE CCCCCCCCCCCCCCC	29.59	23712012
970	Phosphorylation	VKLISGPVRKSTSID EEEECCCCCCCCCCC	15.98	22673903
973	Phosphorylation	ISGPVRKSTSIDTGP ECCCCCCCCCCCCCC	19.47	29779826
974	Phosphorylation	SGPVRKSTSIDTGPT CCCCCCCCCCCCCCC	32.51	27097102
975	Phosphorylation	GPVRKSTSIDTGPTE CCCCCCCCCCCCCCC	26.09	23712012
976	Phosphorylation	PVRKSTSIDTGPTEA CCCCCCCCCCCCCCC	5.79	-
978	Phosphorylation	RKSTSIDTGPTEAPS CCCCCCCCCCCCCCC	43.31	27097102
981	Phosphorylation	TSIDTGPTEAPSSLK CCCCCCCCCCCCHHH	46.23	22673903
985	Phosphorylation	TGPTEAPSSLKRVAS CCCCCCCCHHHHHCC	56.18	27097102
986	Phosphorylation	GPTEAPSSLKRVASP CCCCCCCHHHHHCCC	36.53	27097102
987	Phosphorylation	PTEAPSSLKRVASPA CCCCCCHHHHHCCCC	4.88	-
992	Phosphorylation	SSLKRVASPAAKPAI CHHHHHCCCCCCCCC	16.53	30240740
1061	Phosphorylation	LKDSEGSGTAGKTGS EECCCCCCCCCCCCC	30.37	23984901
1063	Phosphorylation	DSEGSGTAGKTGSLE CCCCCCCCCCCCCCC	22.47	23984901
1066	Phosphorylation	GSGTAGKTGSLEKKP CCCCCCCCCCCCCCH	30.96	23984901
1068	Phosphorylation	GTAGKTGSLEKKPEI CCCCCCCCCCCCHHH	38.29	23984901
1076	Phosphorylation	LEKKPEIAQVIASYT CCCCHHHHHHHHHHC	8.91	23984901
1077	Phosphorylation	EKKPEIAQVIASYTA CCCHHHHHHHHHHCC	32.58	23984901
1078	Phosphorylation	KKPEIAQVIASYTAT CCHHHHHHHHHHCCC	2.67	23984901
1080	Phosphorylation	PEIAQVIASYTATGP HHHHHHHHHHCCCCH	9.53	23984901
1081	Phosphorylation	EIAQVIASYTATGPE HHHHHHHHHCCCCHH	16.34	23984901
1081	Phosphorylation	EIAQVIASYTATGPE HHHHHHHHHCCCCHH	16.34	23984901
1082	Phosphorylation	IAQVIASYTATGPEQ HHHHHHHHCCCCHHH	7.44	23984901
1083	Phosphorylation	AQVIASYTATGPEQL HHHHHHHCCCCHHHE	18.43	23984901
1085	Phosphorylation	VIASYTATGPEQLTL HHHHHCCCCHHHEEE	45.53	23984901
1086	Phosphorylation	IASYTATGPEQLTLA HHHHCCCCHHHEEEC	22.68	23984901
1091	Phosphorylation	ATGPEQLTLAPGQLI CCCHHHEEECCCEEE	22.14	23984901
1129	Phosphorylation	IGWFPANYVKLLSPG EEEEEHHHEEEECCC	10.89	22673903
1134	Phosphorylation	ANYVKLLSPGTSKIT HHHEEEECCCCCCCC	31.55	30411139
1136	Phosphorylation	YVKLLSPGTSKITPT HEEEECCCCCCCCCC	38.96	22673903
1137	Phosphorylation	VKLLSPGTSKITPTE EEEECCCCCCCCCCC	30.17	27097102
1138	Phosphorylation	KLLSPGTSKITPTEL EEECCCCCCCCCCCC	28.16	27097102
1141	Phosphorylation	SPGTSKITPTELPKT CCCCCCCCCCCCCCC	28.09	30411139
1143	Phosphorylation	GTSKITPTELPKTAV CCCCCCCCCCCCCCC	41.31	28689409
1326	Phosphorylation	-------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------		-
1326	Phosphorylation	-------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------		22668510
1662	Phosphorylation	-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		-
1662	Phosphorylation	-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ITSN1_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ITSN1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ITSN1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.