ITK_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ITK_MOUSE
• UniProt AC: Q03526
• Protein Name: Tyrosine-protein kinase ITK/TSK
• Gene Name: Itk
• Organism: Mus musculus (Mouse).
• Sequence Length: 625
• Subcellular Localization: Cytoplasm. Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation..
• Protein Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3. [PubMed: 15662016]
• Protein Sequence: MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQTLVYLQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	SQQKRRTSPSNFKVR HHHHCCCCCCCCEEE	25.23	28285833
166	Phosphorylation	SKKPLPPTPEDNRRS CCCCCCCCCCCCCCC	37.05	27600695
186	Phosphorylation	ETLVIALYDYQTNDP CEEEEEEEECCCCCH	12.00	22817900
517	Phosphorylation	RFVLDDQYTSSTGTK EEEECCCCCCCCCCC	18.65	20438120
568	Phosphorylation	KIPYENRSNSEVVED CCCCCCCCCCCEEEE	57.18	27566939
570	Phosphorylation	PYENRSNSEVVEDIS CCCCCCCCCEEEEHH	32.77	25266776

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
186	Y	Phosphorylation	Kinase	ITK	Q03526	PSP
517	Y	Phosphorylation	Kinase	LCK	P06240	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ITK_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ITK_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of ITK_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.