ITB3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ITB3_MOUSE
• UniProt AC: O54890
• Protein Name: Integrin beta-3
• Gene Name: Itgb3
• Organism: Mus musculus (Mouse).
• Sequence Length: 787
• Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane . Cell junction, focal adhesion .
• Protein Description: Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets. [PubMed: 19332769 ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (By similarity ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (By similarity ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (By similarity]
• Protein Sequence: MRAQWPGQLWAALLALGALAGVVVGESNICTTRGVNSCQQCLAVSPVCAWCSDETLSQGSPRCNLKENLLKDNCAPESIEFPVSEAQILEARPLSSKGSGSSAQITQVSPQRIALRLRPDDSKIFSLQVRQVEDYPVDIYYLMDLSFSMKDDLSSIQTLGTKLASQMRKLTSNLRIGFGAFVDKPVSPYMYISPPQAIKNPCYNMKNACLPMFGYKHVLTLTDQVSRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVLPNDGHCHIGTDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVSLYQNYSELIPGTTVGVLSDDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCVGLKIGDTVSFSIEAKVRGCPQEKEQSFTIKPVGFKDSLTVQVTFDCDCACQAFAQPSSPRCNNGNGTFECGVCRCDQGWLGSMCECSEEDYRPSQQEECSPKEGQPICSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCNCGDCVCDSDWTGYYCNCTTRTDTCMSTNGLLCSGRGNCECGSCVCVQPGSYGDTCEKCPTCPDACSFKKECVECKKFNRGTLHEENTCSRYCRDDIEQVKELTDTGKNAVNCTYKNEDDCVVRFQYYEDTSGRAVLYVVEEPECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
345	N-linked_Glycosylation	NVVSLYQNYSELIPG HHHHHHHHHHHHCCC	28.83	-
396	N-linked_Glycosylation	EELSLSFNATCLNNE CHHEEEEEEEECCCC	30.85	-
477	N-linked_Glycosylation	SPRCNNGNGTFECGV CCCCCCCCCEEEECE	48.75	-
584	N-linked_Glycosylation	DWTGYYCNCTTRTDT CCCEEECCCCCCCCC	15.28	-
679	N-linked_Glycosylation	DTGKNAVNCTYKNED HCCCCEECCEECCCC	15.25	-
766	Phosphorylation	RARAKWDTANNPLYK HHHHHHCCCCCHHHH	30.12	22817900
772	Phosphorylation	DTANNPLYKEATSTF CCCCCHHHHCHHHCC	14.19	22817900
773	Ubiquitination	TANNPLYKEATSTFT CCCCHHHHCHHHCCC	48.86	-
778	Phosphorylation	LYKEATSTFTNITYR HHHCHHHCCCCCEEC	30.08	-
784	Phosphorylation	STFTNITYRGT---- HCCCCCEECCC----	11.84	14519852

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ITB3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ITB3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ITB3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PA24A_MOUSE	Pla2g4a	physical	18840708
KPCB_MOUSE	Prkcb	physical	18840708

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.