ISG15_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ISG15_MOUSE
• UniProt AC: Q64339
• Protein Name: Ubiquitin-like protein ISG15
• Gene Name: Isg15
• Organism: Mus musculus (Mouse).
• Sequence Length: 161
• Subcellular Localization: Cytoplasm. Secreted. Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins..
• Protein Description: Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity..
• Protein Sequence: MAWDLKVKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGGGGDQCA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Ubiquitination	--MAWDLKVKMLGGN --CCCEEEEEEECCC	37.02	-
6	Acetylation	--MAWDLKVKMLGGN --CCCEEEEEEECCC	37.02	22826441
20	Phosphorylation	NDFLVSVTNSMTVSE CCEEEEEECCCCHHH	17.67	28576409
35	Ubiquitination	LKKQIAQKIGVPAFQ HHHHHHHHHCCHHHH	32.19	-
76	S-nitrosocysteine	VMLVVQNCSEPLSIL EEEEEECCCCCEEEE	2.45	-
76	S-nitrosylation	VMLVVQNCSEPLSIL EEEEEECCCCCEEEE	2.45	18606809
106	Ubiquitination	TQTVDTLKKKVSQRE HHHHHHHHHHHHHHH	53.28	-
141	Ubiquitination	LLGEYGLKPQCTVIK HHHHCCCCCCCEEEE	28.78	-
141	Acetylation	LLGEYGLKPQCTVIK HHHHCCCCCCCEEEE	28.78	22826441
144	S-nitrosocysteine	EYGLKPQCTVIKHLR HCCCCCCCEEEEEEE	4.44	-
144	S-nitrosylation	EYGLKPQCTVIKHLR HCCCCCCCEEEEEEE	4.44	18606809
148	Ubiquitination	KPQCTVIKHLRLRGG CCCCEEEEEEEECCC	32.05	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ISG15_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ISG15_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ISG15_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBP5_MOUSE	Usp5	physical	14673145

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.