dbPTM

IMPCT_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IMPCT_MOUSE
UniProt AC	O55091
Protein Name	Protein IMPACT
Gene Name	Impact
Organism	Mus musculus (Mouse).
Sequence Length	318
Subcellular Localization	Cytoplasm .
Protein Description	Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. [PubMed: 15937339]
Protein Sequence	MAEEEVGNSQRQSEEIEAMAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	AEEEVGNSQRQSEEI CHHHCCCCHHHHHHH	22.32	22006019
38	S-nitrosocysteine	DENAKIFCIRVTDFM ECCCCEEEEEECCCC	1.90	-
38	S-nitrosylation	DENAKIFCIRVTDFM ECCCCEEEEEECCCC	1.90	21278135
98	Ubiquitination	EIYVHNMGESILYQW EEEHHCCCHHHHHHH	30.71	27667366
116	Ubiquitination	IRDALIQKSQITEPD HHHHHHHHHHCCCCC	37.02	27667366
130	Phosphorylation	DPDVKKKTEEVEVES CCCHHHHCEEEEECC	46.38	24925903
137	Phosphorylation	TEEVEVESEEDPILE CEEEEECCCCCCCCC	52.00	24925903
159	Phosphorylation	KTLDLKISEETQPET CEEEEEECCCCCCCC	28.00	25338131
204	Ubiquitination	QVKLVLAKLYENKKI HHHHHHHHHHCCCCH	47.65	22790023
206	Phosphorylation	KLVLAKLYENKKIAS HHHHHHHHCCCCHHH	19.42	20139300
210	Ubiquitination	AKLYENKKIASATHN HHHHCCCCHHHCCCC	55.90	22790023
213	Phosphorylation	YENKKIASATHNIYA HCCCCHHHCCCCEEE	36.85	20139300
215	Phosphorylation	NKKIASATHNIYAYR CCCHHHCCCCEEEEE	16.98	20139300
219	Phosphorylation	ASATHNIYAYRIFCE HHCCCCEEEEEEEEC	11.69	20139300
221	Phosphorylation	ATHNIYAYRIFCEDK CCCCEEEEEEEECCH	6.30	20139300
228	Ubiquitination	YRIFCEDKQTFLQDC EEEEECCHHHEECCC	29.01	22790023
284	Ubiquitination	FKHINNCARNILVEK HHHHHHHHHHHHEEC	15.07	27667366
291	Ubiquitination	ARNILVEKNFTNTPD HHHHHEECCCCCCCC	50.29	22790023
296	Phosphorylation	VEKNFTNTPDESTKN EECCCCCCCCHHHHC	29.11	25521595
302	Ubiquitination	NTPDESTKNLGKKKV CCCCHHHHCCCCHHC	60.50	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of IMPCT_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of IMPCT_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IMPCT_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
CDK1_YEAST	CDC28	physical	26176233
CDK1_MOUSE	Cdk1	physical	26176233

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IMPCT_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND MASSSPECTROMETRY.	19131326 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND MASSSPECTROMETRY.	17203969 [Abstract]