ILF3_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ILF3_RAT
• UniProt AC: Q9JIL3
• Protein Name: Interleukin enhancer-binding factor 3
• Gene Name: Ilf3
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 897
• Subcellular Localization: Nucleus, nucleolus . Cytoplasm . Nucleus . Localizes in the cytoplasm in response to viral infection. The unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-I
• Protein Description: RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs..
• Protein Sequence: MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGNSELSEAENMDTPPDDESKEGAGEQKAEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLTTVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLISQTGPVHAPIFTMSVEVDGSTFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEESDGKPAVVAPPPVVEAVSNPSSVFPSDATTEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDAPLALEANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEAPPPPNIRGRGRGGNIRGRGRGRGFGGTNHGGGYMNAGAGYGSYGYSSNSATAGYSQFYSNGGHYGNAGGGGSGGGGGSSSYSSYYQGDSYNSPVPPKHAGKKPLHGGQQKPSYSSGYQSHQGQQQPYNQSQYSSYGTPQGKQKGYGHGQGSYSSYSNSYNSPGGGGGSDYSYDSKFNYSGSGGRSGGNSYGSSGSSYNTGSHGGYGAGSGGSSSYQGKQGGYSSQSNYSSPGSSQSYSGPASSYQSSQGGYSRNTEHSMNYQYR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
19	Phosphorylation	RHVMAKHSSVYPTQE CCHHHHCCCCCCCHH	22.31	25575281
20	Phosphorylation	HVMAKHSSVYPTQEE CHHHHCCCCCCCHHH	25.95	25575281
22	Phosphorylation	MAKHSSVYPTQEELE HHHCCCCCCCHHHHH	11.51	25575281
24	Phosphorylation	KHSSVYPTQEELEAV HCCCCCCCHHHHHHH	30.81	25575281
36	Phosphorylation	EAVQNMVSHTERALK HHHHHHHHHHHHHHH	17.58	25575281
38	Phosphorylation	VQNMVSHTERALKAV HHHHHHHHHHHHHHH	21.47	25575281
57	Phosphorylation	DEQEKGNSELSEAEN HHHHCCCCCCHHHHH	48.89	22673903
60	Phosphorylation	EKGNSELSEAENMDT HCCCCCCHHHHHCCC	30.61	23298284
67	Phosphorylation	SEAENMDTPPDDESK HHHHHCCCCCCCCCC	26.42	23298284
73	Phosphorylation	DTPPDDESKEGAGEQ CCCCCCCCCCCCCHH	42.89	23298284
100	Acetylation	MRVGLVAKGLLLKGD HHHHHHHHCHHHCCC	42.48	-
100	Ubiquitination	MRVGLVAKGLLLKGD HHHHHHHHCHHHCCC	42.48	-
144	Phosphorylation	TTVTEDKYEILQSVD EEECCCHHHHHHHCC	22.11	-
188	Phosphorylation	EKVLAGETLSVNDPP HHHHCCCCCCCCCCC	24.34	-
190	Phosphorylation	VLAGETLSVNDPPDV HHCCCCCCCCCCCCC	26.71	23984901
209	Phosphorylation	KCLAALASLRHAKWF HHHHHHHHHHHHHHH	27.13	23984901
214	Acetylation	LASLRHAKWFQARAN HHHHHHHHHHHHHHC	42.27	22902405
315	Phosphorylation	RQQREDITQSAQHAL HHHHHHHHHHHHHHH	28.88	-
382	Phosphorylation	EEDGEEKSPSKKKKK CCCCCCCCCCHHHHH	37.37	30411139
384	Phosphorylation	DGEEKSPSKKKKKIQ CCCCCCCCHHHHHHH	65.29	27097102
460	Acetylation	AKLHVAVKVLQDMGL HHHHHHHHHHHHCCC	27.73	-
469	Phosphorylation	LQDMGLPTGAEGRDS HHHCCCCCCCCCCCC	54.81	27097102
476	Phosphorylation	TGAEGRDSSKGEDSA CCCCCCCCCCCCCCC	32.37	27097102
477	Phosphorylation	GAEGRDSSKGEDSAE CCCCCCCCCCCCCCC	49.31	27097102
482	Phosphorylation	DSSKGEDSAEESDGK CCCCCCCCCCCCCCC	33.02	27097102
486	Phosphorylation	GEDSAEESDGKPAVV CCCCCCCCCCCCCEE	43.07	27097102
503	Phosphorylation	PPVVEAVSNPSSVFP CCCEEECCCCCCCCC	50.50	27097102
506	Phosphorylation	VEAVSNPSSVFPSDA EEECCCCCCCCCCCC	43.30	27097102
507	Phosphorylation	EAVSNPSSVFPSDAT EECCCCCCCCCCCCC	29.09	27097102
511	Phosphorylation	NPSSVFPSDATTEQG CCCCCCCCCCCCCCC	28.54	28432305
514	Phosphorylation	SVFPSDATTEQGPIL CCCCCCCCCCCCCCC	35.54	28432305
515	Phosphorylation	VFPSDATTEQGPILT CCCCCCCCCCCCCCC	28.21	28432305
522	Phosphorylation	TEQGPILTKHGKNPV CCCCCCCCCCCCCCC	23.01	28432305
571	Phosphorylation	QKFQGAGSNKKVAKA EEECCCCCCHHHHHH	44.96	21630457
609	Methylation	KRAPVPVRGGPKFAA CCCCCCCCCCCCCCC	37.98	19019797
778	Phosphorylation	PQGKQKGYGHGQGSY CCCCCCCCCCCCCCC	17.00	23984901
784	Phosphorylation	GYGHGQGSYSSYSNS CCCCCCCCCCCCCCC	17.60	23984901
785	Phosphorylation	YGHGQGSYSSYSNSY CCCCCCCCCCCCCCC	14.41	23984901
786	Phosphorylation	GHGQGSYSSYSNSYN CCCCCCCCCCCCCCC	25.21	23984901
787	Phosphorylation	HGQGSYSSYSNSYNS CCCCCCCCCCCCCCC	25.09	23984901
788	Phosphorylation	GQGSYSSYSNSYNSP CCCCCCCCCCCCCCC	13.11	23984901
789	Phosphorylation	QGSYSSYSNSYNSPG CCCCCCCCCCCCCCC	22.65	23984901
791	Phosphorylation	SYSSYSNSYNSPGGG CCCCCCCCCCCCCCC	21.54	23984901
792	Phosphorylation	YSSYSNSYNSPGGGG CCCCCCCCCCCCCCC	24.74	23984901
794	Phosphorylation	SYSNSYNSPGGGGGS CCCCCCCCCCCCCCC	19.38	23984901
801	Phosphorylation	SPGGGGGSDYSYDSK CCCCCCCCCCCCCCC	36.33	23984901
803	Phosphorylation	GGGGGSDYSYDSKFN CCCCCCCCCCCCCCC	15.72	23984901
804	Phosphorylation	GGGGSDYSYDSKFNY CCCCCCCCCCCCCCC	27.70	23984901
805	Phosphorylation	GGGSDYSYDSKFNYS CCCCCCCCCCCCCCC	20.29	23984901
811	Phosphorylation	SYDSKFNYSGSGGRS CCCCCCCCCCCCCCC	19.96	27097102
812	Phosphorylation	YDSKFNYSGSGGRSG CCCCCCCCCCCCCCC	27.82	23984901
814	Phosphorylation	SKFNYSGSGGRSGGN CCCCCCCCCCCCCCC	31.94	28432305
818	Phosphorylation	YSGSGGRSGGNSYGS CCCCCCCCCCCCCCC	55.09	28689409
822	Phosphorylation	GGRSGGNSYGSSGSS CCCCCCCCCCCCCCC	33.86	28689409
834	Phosphorylation	GSSYNTGSHGGYGAG CCCCCCCCCCCCCCC	19.54	28689409
867	Phosphorylation	NYSSPGSSQSYSGPA CCCCCCCCCCCCCCC	29.12	28689409
891	Phosphorylation	YSRNTEHSMNYQYR- CCCCCCCCCCCCCC-	11.51	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
188	T	Phosphorylation	Kinase	PKR	-	Uniprot
315	T	Phosphorylation	Kinase	PKR	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
188	T	Phosphorylation	Phosphorylated at Thr-188 and Thr-315 by PKR in response to RNA viruses.	-
315	T	Phosphorylation	Phosphorylated at Thr-188 and Thr-315 by PKR in response to RNA viruses.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ILF3_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of ILF3_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.