IKBA_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IKBA_RAT
• UniProt AC: Q63746
• Protein Name: NF-kappa-B inhibitor alpha
• Gene Name: Nfkbia
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 314
• Subcellular Localization: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export..
• Protein Description: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (By similarity)..
• Protein Sequence: MFQPAGHGQDWAMEGPRDGLKKERLVDDRHDSGLDSMKDEDYEQMVKELREIRLQPQEAPLAAEPWKQQLTEDGDSFLHLAIIHEEKTLTMEVIGQVKGDLAFLNFQNNLQQTPLHLAVITNQPGIAEALLKAGCDPELRDFRGNTPLHLACEQGCLASVAVLTQTCTPQHLHSVLQATNYNGHTCLHLASIHGYLGIVEHLVTLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQTLPESEDEESYDTESEFTEDELPYDDCVFGGQRLTL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Phosphorylation	LVDDRHDSGLDSMKD CCCCCCCCCHHHCCH	34.82	11490015
36	Phosphorylation	RHDSGLDSMKDEDYE CCCCCHHHCCHHHHH	32.54	16407217
42	Phosphorylation	DSMKDEDYEQMVKEL HHCCHHHHHHHHHHH	13.15	15935065
210	Hydroxylation	VTLGADVNAQEPCNG HHCCCCCCCCCCCCC	36.13	-
244	Hydroxylation	LKCGADVNRVTYQGY HHCCCCCCCEEECCC	33.00	-
283	Phosphorylation	NLQTLPESEDEESYD HHCCCCCCCCCCCCC	48.70	-
288	Phosphorylation	PESEDEESYDTESEF CCCCCCCCCCCCCCC	26.68	-
291	Phosphorylation	EDEESYDTESEFTED CCCCCCCCCCCCCCC	32.93	-
293	Phosphorylation	EESYDTESEFTEDEL CCCCCCCCCCCCCCC	39.85	-
296	Phosphorylation	YDTESEFTEDELPYD CCCCCCCCCCCCCCC	38.18	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
32	S	Phosphorylation	Kinase	IKBKB	Q9QY78	GPS
32	S	Phosphorylation	Kinase	RPS6KA1	Q63531	GPS
32	S	Phosphorylation	Kinase	IKKA	-	Uniprot
36	S	Phosphorylation	Kinase	IKBKB	Q9QY78	GPS
36	S	Phosphorylation	Kinase	RPS6KA1	Q63531	GPS
36	S	Phosphorylation	Kinase	IKKA	-	Uniprot
36	S	Phosphorylation	Kinase	IKKE	-	Uniprot
36	S	Phosphorylation	Kinase	TBK1	-	Uniprot
42	Y	Phosphorylation	Kinase	TYR-KINASES	-	Uniprot
283	S	Phosphorylation	Kinase	CK2	-	Uniprot
288	S	Phosphorylation	Kinase	CK2	-	Uniprot
291	T	Phosphorylation	Kinase	CK2	-	Uniprot
293	S	Phosphorylation	Kinase	CK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
21	K	ubiquitylation	Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3.	-
22	K	ubiquitylation	Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3.	-
32	S	Phosphorylation	Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36 (By similarity).	-
32	S	ubiquitylation	Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36 (By similarity).	-
36	S	Phosphorylation	Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36 (By similarity).	-
36	S	ubiquitylation	Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36 (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IKBA_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CXA1_MOUSE	Gja1	physical	15777848

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.