IF4A3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IF4A3_MOUSE
• UniProt AC: Q91VC3
• Protein Name: Eukaryotic initiation factor 4A-III
• Gene Name: Eif4a3
• Organism: Mus musculus (Mouse).
• Sequence Length: 411
• Subcellular Localization: Nucleus . Nucleus speckle . Cytoplasm . Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments
• Protein Description: ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly. Involved in craniofacial development..
• Protein Sequence: MAANATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSVSVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MAANATMA -------CCCCCCCC	7.78	-
2	Acetylation	------MAANATMAT ------CCCCCCCCC	14.93	-
6	Phosphorylation	--MAANATMATSGSA --CCCCCCCCCCHHH	13.72	25367039
9	Phosphorylation	AANATMATSGSARKR CCCCCCCCCHHHHHH	23.85	29233185
10	Phosphorylation	ANATMATSGSARKRL CCCCCCCCHHHHHHH	22.63	27566939
12	Phosphorylation	ATMATSGSARKRLLK CCCCCCHHHHHHHHC	25.35	26824392
24	Phosphorylation	LLKEEDMTKVEFETS HHCHHHCCCEEEECC	44.23	20469934
54	Phosphorylation	EDLLRGIYAYGFEKP HHHHHHHHHHCCCCC	9.15	22499769
56	Phosphorylation	LLRGIYAYGFEKPSA HHHHHHHHCCCCCHH	12.84	22499769
60	Acetylation	IYAYGFEKPSAIQQR HHHHCCCCCHHHHHH	41.85	22642817
60	Ubiquitination	IYAYGFEKPSAIQQR HHHHCCCCCHHHHHH	41.85	27667366
124	Acetylation	ELAVQIQKGLLALGD HHHHHHHHHHHHHCC	53.83	-
163	Phosphorylation	GQHVVAGTPGRVFDM CCEEECCCCCHHHHH	16.91	26824392
198	Acetylation	EMLNKGFKEQIYDVY HHHHHHHHHHHHHHH	58.33	22826441
202	Phosphorylation	KGFKEQIYDVYRYLP HHHHHHHHHHHHHCC	10.07	18563927
242	Acetylation	DPIRILVKRDELTLE CCEEEEECCCCCCHH	51.16	23864654
252	Ubiquitination	ELTLEGIKQFFVAVE CCCHHHHHHHEEEEE	52.49	-
289	Acetylation	IFCNTKRKVDWLTEK HHCCCCCCHHHHHHH	46.21	22826441
289	Ubiquitination	IFCNTKRKVDWLTEK HHCCCCCCHHHHHHH	46.21	-
296	Acetylation	KVDWLTEKMREANFT CHHHHHHHHHHCCCE	37.71	-
321	Acetylation	KERESIMKEFRSGAS HHHHHHHHHHHHCCC	52.62	-
374	Ubiquitination	RSGRYGRKGVAINFV CCCCCCCCCEEEEEE	54.06	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of IF4A3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IF4A3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IF4A3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IF4A3_HUMAN	EIF4A3	physical	20360068

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.