HNF4A_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HNF4A_RAT
• UniProt AC: P22449
• Protein Name: Hepatocyte nuclear factor 4-alpha
• Gene Name: Hnf4a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 474
• Subcellular Localization: Nucleus.
• Protein Description: Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine..
• Protein Sequence: MRLSKTLADMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
87	Phosphorylation	CKGFFRRSVRKNHMY CCHHHHHHHHCCCCE	23.24	17389749
142	Phosphorylation	DRISTRRSSYEDSSL HHHHHCHHHCCCCCC	33.70	22673903
143	Phosphorylation	RISTRRSSYEDSSLP HHHHCHHHCCCCCCH	30.63	22673903
144	Phosphorylation	ISTRRSSYEDSSLPS HHHCHHHCCCCCCHH	25.63	23984901
147	Phosphorylation	RRSSYEDSSLPSINA CHHHCCCCCCHHHHH	23.10	23984901
148	Phosphorylation	RSSYEDSSLPSINAL HHHCCCCCCHHHHHH	57.53	23984901
151	Phosphorylation	YEDSSLPSINALLQA CCCCCCHHHHHHHHH	33.49	23984901
162	Phosphorylation	LLQAEVLSQQITSPI HHHHHHHHHHCCCCC	25.80	22673903
166	Phosphorylation	EVLSQQITSPISGIN HHHHHHCCCCCCCCC	24.90	22673903
167	Phosphorylation	VLSQQITSPISGING HHHHHCCCCCCCCCC	23.12	22673903
170	Phosphorylation	QQITSPISGINGDIR HHCCCCCCCCCCCHH	36.68	22673903
313	Phosphorylation	GKIKRLRSQVQVSLE HHHHHHHHHEEEEHH	39.02	-
429	Phosphorylation	RPRGQAATPETPQPS CCCCCCCCCCCCCCC	25.36	28432305
432	Phosphorylation	GQAATPETPQPSPPS CCCCCCCCCCCCCCC	28.55	28432305
436	Phosphorylation	TPETPQPSPPSGSGS CCCCCCCCCCCCCCC	44.17	21738781
439	Phosphorylation	TPQPSPPSGSGSESY CCCCCCCCCCCCCCE	49.31	28432305
441	Phosphorylation	QPSPPSGSGSESYKL CCCCCCCCCCCCEEE	43.41	28432305
443	Phosphorylation	SPPSGSGSESYKLLP CCCCCCCCCCEEECC	25.68	28689409
445	Phosphorylation	PSGSGSESYKLLPGA CCCCCCCCEEECCCC	29.30	28689409
446	Phosphorylation	SGSGSESYKLLPGAI CCCCCCCEEECCCCE	11.12	28689409
458	Acetylation	GAITTIVKPPSAIPQ CCEEEEECCCCCCCC	47.57	22902405

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
87	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
87	S	Phosphorylation	Kinase	PRKCB	P05771	GPS
87	S	Phosphorylation	Kinase	PRKCG	P05129	GPS
142	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
142	S	Phosphorylation	Kinase	PKA	-	Uniprot
143	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
143	S	Phosphorylation	Kinase	PKA	-	Uniprot
313	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
313	S	Phosphorylation	Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity).	9234678
458	K	Acetylation	Acetylation at Lys-458 lowers transcriptional activation by about two-fold.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HNF4A_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of HNF4A_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.