HMGB3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HMGB3_MOUSE
• UniProt AC: O54879
• Protein Name: High mobility group protein B3
• Gene Name: Hmgb3
• Organism: Mus musculus (Mouse).
• Sequence Length: 200
• Subcellular Localization: Nucleus . Chromosome . Cytoplasm .
• Protein Description: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. [PubMed: 19890330 Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling]
• Protein Sequence: MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSSKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLSDNEKQPYVTKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEEEEEEEEEEEEEEEEDE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Acetylation	-----MAKGDPKKPK -----CCCCCCCCCC	63.65	-
12	Acetylation	DPKKPKGKMSAYAFF CCCCCCCCHHHHHHH	35.49	23806337
23	Cysteine derivative	YAFFVQTCREEHKKK HHHHHHHHHHHHHHH	2.63	-
23	Oxidation	YAFFVQTCREEHKKK HHHHHHHHHHHHHHH	2.63	-
30	Acetylation	CREEHKKKNPEVPVN HHHHHHHHCCCCCCC	80.62	-
42	Phosphorylation	PVNFAEFSKKCSERW CCCHHHHHHHHHHHH	23.78	22817900
43	Acetylation	VNFAEFSKKCSERWK CCHHHHHHHHHHHHH	64.22	22826441
45	Cysteine derivative	FAEFSKKCSERWKTM HHHHHHHHHHHHHHC	6.16	-
45	Oxidation	FAEFSKKCSERWKTM HHHHHHHHHHHHHHC	6.16	-
95	Methylation	KDPNAPKRPPSGFFL CCCCCCCCCCCCCEE	47.50	58857841
98	Phosphorylation	NAPKRPPSGFFLFCS CCCCCCCCCCEEEEC	51.41	24719451
104	Oxidation	PSGFFLFCSEFRPKI CCCCEEEECCCCCCC	4.08	-
104	Cysteine derivative	PSGFFLFCSEFRPKI CCCCEEEECCCCCCC	4.08	-
104	Glutathionylation	PSGFFLFCSEFRPKI CCCCEEEECCCCCCC	4.08	24333276
112	Acetylation	SEFRPKIKSTNPGIS CCCCCCCCCCCCCCC	58.30	-
113	Phosphorylation	EFRPKIKSTNPGISI CCCCCCCCCCCCCCH	36.57	22817900
114	Phosphorylation	FRPKIKSTNPGISIG CCCCCCCCCCCCCHH	40.19	20139300
125	Acetylation	ISIGDVAKKLGEMWN CCHHHHHHHHHHHHH	48.67	7627571
126	Acetylation	SIGDVAKKLGEMWNN CHHHHHHHHHHHHHC	52.57	23806337
135	Phosphorylation	GEMWNNLSDNEKQPY HHHHHCCCCCCCCCH	40.28	27087446
139	Acetylation	NNLSDNEKQPYVTKA HCCCCCCCCCHHHHH	63.93	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HMGB3_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
23	C	Oxidation	Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).	-
45	C	Oxidation	Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).	-
104	C	Oxidation	Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HMGB3_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of HMGB3_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.