dbPTM

HMDH_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HMDH_RAT
UniProt AC	P51639
Protein Name	3-hydroxy-3-methylglutaryl-coenzyme A reductase
Gene Name	Hmgcr
Organism	Rattus norvegicus (Rat).
Sequence Length	887
Subcellular Localization	Endoplasmic reticulum membrane Multi-pass membrane protein.
Protein Description	Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins..
Protein Sequence	MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQSKVSLGLAEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAVKYIFFEQAETESTLSLKNPITSPVVTPKKAQDNCCRREPLLVRRNQKLSSVEEDPGVNQDRKVEVIKPLVAEAETSGRATFVLGASAASPPLALGAQEPGIELPSEPRPNEECLQILESAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSAKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMSRGPVVRLPRACDSAEVKSWLETPEGFAVVKEAFDSTSRFARLQKLHVTLAGRNLYIRLQSKTGDAMGMNMISKGTEKALLKLQEFFPELQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
248	Ubiquitination	VLEEEENKPNPVTQR HHHHHHCCCCCHHHH	49.83	-
281	N-linked_Glycosylation	IADPSPQNSTAEQSK CCCCCCCCCCHHHHC	44.72	-
355	Phosphorylation	LSLKNPITSPVVTPK ECCCCCCCCCCCCCC	28.38	27097102
356	Phosphorylation	SLKNPITSPVVTPKK CCCCCCCCCCCCCCH	19.20	27097102
360	Phosphorylation	PITSPVVTPKKAQDN CCCCCCCCCCHHHCC	29.62	27097102
383	Phosphorylation	VRRNQKLSSVEEDPG HHCCCCCCCCCCCCC	39.10	28432305
384	Phosphorylation	RRNQKLSSVEEDPGV HCCCCCCCCCCCCCC	43.94	28432305
871	Phosphorylation	SHMVHNRSKINLQDL HHCCCCCCCCCHHHH	43.11	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
871	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot
871	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
871	S	Phosphorylation	Kinase	CAMK1	Q63450	GPS
871	S	Phosphorylation	Kinase	PRKCA	P05696	GPS
871	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
871	S	Phosphorylation	Kinase	AMPK_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
248	K	ubiquitylation	-
Lys-248 is the main site of ubiquitination.
871	S	Phosphorylation	2369897
Phosphorylation at Ser-871 reduces the catalytic activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HMDH_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of HMDH_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HMDH_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Regulation of HMG-CoA reductase: identification of the sitephosphorylated by the AMP-activated protein kinase in vitro and inintact rat liver."; Clarke P.R., Hardie D.G.; EMBO J. 9:2439-2446(1990). Cited for: PROTEIN SEQUENCE OF 423-443 AND 867-878, AND PHOSPHORYLATION ATSER-871.	2369897 [Abstract]