dbPTM

HIPK3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HIPK3_MOUSE
UniProt AC	Q9ERH7
Protein Name	Homeodomain-interacting protein kinase 3
Gene Name	Hipk3
Organism	Mus musculus (Mouse).
Sequence Length	1192
Subcellular Localization	Cytoplasm. Nucleus.
Protein Description	Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE)..
Protein Sequence	MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSGCVFQERTYPQIHVNGRNFGNSHPSTKGSAFQTKIPFTKPRGHSFSLQAGAIVVKDTAGATKVLAAQAQQAGVEAPRAVVWRNRLHFLEGPQRCGLKRKSEELENHSGAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTSGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPVLQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCRETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDIVHVNTVMDLEGGDLLAEKADRREFVNLLKKMLLIDADLRITPIETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSPSSCETNNHSKMSLLRPVASNGTAALAANFTKVGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFHQTLIICPPAIQGIPAAHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQQTWSGRTQQMLIPAWQQVTPMAPAAATLTSEGMAGSQRLGDWGKMIPHSNHYNSVMPPPLLTNQITLSAPQPISVGIAHVVWPQPATTKKNKLCQNRSNSLQNTNIPHSAFISPKIISGKEVEEVSCVDTQDNHTSEGEAGTCREASVRQDSSVSDKQRQTIIIADSPSPAVSVITISSDSDDEETSPRPSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDDEQESGCETVDGSPTSDSSGHDSPFAENSFVEDAHQNTELGTCAGPEAKPAVGTAVEPPVGRESGLSVDEHMANTDSTCQPLRKGQPAPGKLHQPPALGARQQKPAAAFPQQHLNLSQVQHFGTGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGSTHLGGQPTLLPYPSSASLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGINPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
357	Phosphorylation	HVSKTVCSTYLQSRY CCCHHHHHHHHHHCC	18.75	22499769
358	Phosphorylation	VSKTVCSTYLQSRYY CCHHHHHHHHHHCCC	24.11	22499769
359	Phosphorylation	SKTVCSTYLQSRYYR CHHHHHHHHHHCCCC	6.10	25521595
362	Phosphorylation	VCSTYLQSRYYRAPE HHHHHHHHCCCCCCH	21.47	21082442
428	Ubiquitination	QLLNVGTKSTRFFCR HEEECCCCCCEEEEC	43.85	-
770	Phosphorylation	NKLCQNRSNSLQNTN CHHHCCCCCCCCCCC	38.02	26643407
772	Phosphorylation	LCQNRSNSLQNTNIP HHCCCCCCCCCCCCC	32.36	26643407
776	Phosphorylation	RSNSLQNTNIPHSAF CCCCCCCCCCCCHHC	22.89	29899451
870	Phosphorylation	SLRECKGSLDCEACQ CHHHCCCCCCHHHHH	13.41	-
1000	Acetylation	STCQPLRKGQPAPGK CCCCCCCCCCCCCCC	70.36	7718731
1182	Phosphorylation	AYTGFPLSPTKLSQY CCCCCCCCCCCHHHC	30.66	20415495
1184	Phosphorylation	TGFPLSPTKLSQYPY CCCCCCCCCHHHCCC	41.04	20415495

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of HIPK3_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of HIPK3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HIPK3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of HIPK3_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HIPK3_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-359, AND MASSSPECTROMETRY.	18034455 [Abstract]