HCN2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HCN2_RAT
• UniProt AC: Q9JKA9
• Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
• Gene Name: Hcn2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 863
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein .
• Protein Description: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current..
• Protein Sequence: MDARGGGGRPGDSPGTTPAPGPPPPPPPPAPLQLQPPPAPPPNPTTPSHPESADEPGPRSRLCSRDSSCTPGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPAAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGFFPPPPPRQVRSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPSLPHGAPAPSPAASARPASSSTPRLGPAPTTRTAAPSPDRRDSASPGAASGLDPLDSARSRLSSNL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Phosphorylation	GPRSRLCSRDSSCTP CCCHHCCCCCCCCCC	43.65	28551015
67	Phosphorylation	SRLCSRDSSCTPGAA HHCCCCCCCCCCCCC	26.63	28551015
68	Phosphorylation	RLCSRDSSCTPGAAK HCCCCCCCCCCCCCC	26.71	28551015
70	Phosphorylation	CSRDSSCTPGAAKGG CCCCCCCCCCCCCCC	27.06	28551015
90	Phosphorylation	GRGEPQCSPEGPARG CCCCCCCCCCCCCCC	22.27	28551015
119	Phosphorylation	AAAEEAGSEEAGPAG HHHHHHCCCCCCCCC	39.03	30240740
131	Phosphorylation	PAGEPRGSQASFLQR CCCCCCCHHHHHHHH	24.69	22673903
134	Phosphorylation	EPRGSQASFLQRQFG CCCCHHHHHHHHHHH	20.89	22673903
150	Ubiquitination	LLQPGVNKFSLRMFG HHCCCCCHHHHHHHC	33.64	-
160	Ubiquitination	LRMFGSQKAVEREQE HHHHCCHHHHHHHHH	56.98	-
380	N-linked_Glycosylation	VSINNMVNHSWSELY EEHHHHCCCCHHHHH	16.83	-
641	Phosphorylation	DRIGKKNSILLHKVQ HHHCCCCCCCEEECC	24.40	25403869
726	Phosphorylation	VGPLALGSPRLVRRA CCCHHCCCCCCCCCC	14.01	27097102
728	Methylation	PLALGSPRLVRRAPP CHHCCCCCCCCCCCC	47.73	-
743	Phosphorylation	GPLPPAASPGPPAAS CCCCCCCCCCCCCCC	32.28	30322021
750	Phosphorylation	SPGPPAASPPAAPSS CCCCCCCCCCCCCCC	33.26	27097102
756	Phosphorylation	ASPPAAPSSPRAPRT CCCCCCCCCCCCCCC	48.53	27097102
757	Phosphorylation	SPPAAPSSPRAPRTS CCCCCCCCCCCCCCC	19.83	27097102
763	Phosphorylation	SSPRAPRTSPYGVPG CCCCCCCCCCCCCCC	32.75	28551015
764	Phosphorylation	SPRAPRTSPYGVPGS CCCCCCCCCCCCCCC	19.87	28551015
766	Phosphorylation	RAPRTSPYGVPGSPA CCCCCCCCCCCCCCC	30.44	28551015
771	Phosphorylation	SPYGVPGSPATRVGP CCCCCCCCCCCCCCC	12.80	25403869
774	Phosphorylation	GVPGSPATRVGPALP CCCCCCCCCCCCCCC	29.31	28432305
789	Phosphorylation	ARRLSRASRPLSASQ HHHHHCCCCCCCCCC	33.06	22673903
793	Phosphorylation	SRASRPLSASQPSLP HCCCCCCCCCCCCCC	28.67	22673903
795	Phosphorylation	ASRPLSASQPSLPHG CCCCCCCCCCCCCCC	38.97	27115346
798	Phosphorylation	PLSASQPSLPHGAPA CCCCCCCCCCCCCCC	47.07	30240740
816	Phosphorylation	AASARPASSSTPRLG CHHCCCCCCCCCCCC	27.75	16641100
817	Phosphorylation	ASARPASSSTPRLGP HHCCCCCCCCCCCCC	40.10	16641100
830	Phosphorylation	GPAPTTRTAAPSPDR CCCCCCCCCCCCCCC	25.69	28551015
834	Phosphorylation	TTRTAAPSPDRRDSA CCCCCCCCCCCCCCC	34.40	28432305
840	Phosphorylation	PSPDRRDSASPGAAS CCCCCCCCCCCCHHC	28.91	28432305
842	Phosphorylation	PDRRDSASPGAASGL CCCCCCCCCCHHCCC	28.02	30411139
847	Phosphorylation	SASPGAASGLDPLDS CCCCCHHCCCCHHHH	39.08	28551015

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
641	S	Phosphorylation	Kinase	PRKG2	Q64595	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
641	S	Phosphorylation	Phosphorylation at Ser-641 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HCN2_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of HCN2_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.