HAP28_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HAP28_MOUSE
• UniProt AC: Q3UHX2
• Protein Name: 28 kDa heat- and acid-stable phosphoprotein
• Gene Name: Pdap1
• Organism: Mus musculus (Mouse).
• Sequence Length: 181
• Protein Sequence: MPKGGRKGGHKGRVRQYTSPEEIDAQLQAEKQKANEEDEQEEGGDGASGDPKKEKKSLDSDESEDEDDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLDGPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERKAKDDATLSGKRMQSLSLNK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
17	Phosphorylation	HKGRVRQYTSPEEID CCCCCCCCCCHHHHH	9.91	23984901
18	Phosphorylation	KGRVRQYTSPEEIDA CCCCCCCCCHHHHHH	29.48	25521595
19	Phosphorylation	GRVRQYTSPEEIDAQ CCCCCCCCHHHHHHH	25.94	25521595
48	Phosphorylation	EEGGDGASGDPKKEK HCCCCCCCCCCHHHH	49.62	25521595
52	Acetylation	DGASGDPKKEKKSLD CCCCCCCHHHHCCCC	77.60	12563341
53	Acetylation	GASGDPKKEKKSLDS CCCCCCHHHHCCCCC	78.96	12649943
57	Phosphorylation	DPKKEKKSLDSDESE CCHHHHCCCCCCCCC	48.77	27087446
60	Phosphorylation	KEKKSLDSDESEDED HHHCCCCCCCCCCCC	48.87	27087446
63	Phosphorylation	KSLDSDESEDEDDDY CCCCCCCCCCCCCHH	56.17	27087446
70	Phosphorylation	SEDEDDDYQQKRKGV CCCCCCHHHHHHHCC	21.60	27149854
95	Malonylation	RVAQTTKKVTQLDLD HHHHCCCEEEECCCC	48.71	26320211
97	Phosphorylation	AQTTKKVTQLDLDGP HHCCCEEEECCCCCH	32.09	29514104
108	Phosphorylation	LDGPKELSRREREEI CCCHHHHCHHHHHHH	30.31	-
126	Methylation	KAKERYMKMHLAGKT HHHHHHHHHHHCCCH	17.77	-
132	Malonylation	MKMHLAGKTEQAKAD HHHHHCCCHHHHHHH	43.19	26320211
132	Acetylation	MKMHLAGKTEQAKAD HHHHHCCCHHHHHHH	43.19	23806337
137	Malonylation	AGKTEQAKADLARLA CCCHHHHHHHHHHHH	41.97	26320211
164	Acetylation	KEEERKAKDDATLSG HHHHHHHHHHCCCCC	60.21	23806337
168	Phosphorylation	RKAKDDATLSGKRMQ HHHHHHCCCCCHHHH	28.57	22817900
170	Phosphorylation	AKDDATLSGKRMQSL HHHHCCCCCHHHHHH	37.60	26824392
172	Acetylation	DDATLSGKRMQSLSL HHCCCCCHHHHHHCC	41.12	69133
176	Phosphorylation	LSGKRMQSLSLNK-- CCCHHHHHHCCCC--	15.58	27087446
178	Phosphorylation	GKRMQSLSLNK---- CHHHHHHCCCC----	34.83	28725479

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of HAP28_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HAP28_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HAP28_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of HAP28_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-63 AND TYR-70,AND MASS SPECTROMETRY.
PubMed: 19131326

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 AND SER-63, AND MASSSPECTROMETRY.
PubMed: 19367708

"Specific phosphopeptide enrichment with immobilized titanium ionaffinity chromatography adsorbent for phosphoproteome analysis.";
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.;
J. Proteome Res. 7:3957-3967(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57 AND SER-63, AND MASSSPECTROMETRY.
PubMed: 18630941

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-60 AND SER-63,AND MASS SPECTROMETRY.
PubMed: 17242355

"A differential phosphoproteomic analysis of retinoic acid-treated P19cells.";
Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.;
J. Proteome Res. 6:3174-3186(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-60 AND SER-63,AND MASS SPECTROMETRY.
PubMed: 17622165

"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry.";
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
J. Proteome Res. 6:250-262(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48 AND SER-63, AND MASSSPECTROMETRY.
PubMed: 17203969