H2AJ_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: H2AJ_HUMAN
• UniProt AC: Q9BTM1
• Protein Name: Histone H2A.J
• Gene Name: H2AFJ
• Organism: Homo sapiens (Human).
• Sequence Length: 129
• Subcellular Localization: Nucleus. Chromosome.
• Protein Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling..
• Protein Sequence: MSGRGKQGGKVRAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESQKTKSK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSGRGKQGG ------CCCCCCCCC	36.88	21406692
6	Acetylation	--MSGRGKQGGKVRA --CCCCCCCCCCCCH	44.39	158541
6	Lactylation	--MSGRGKQGGKVRA --CCCCCCCCCCCCH	44.39	31645732
6	Ubiquitination	--MSGRGKQGGKVRA --CCCCCCCCCCCCH	44.39	-
10	Ubiquitination	GRGKQGGKVRAKAKS CCCCCCCCCCHHCCC	35.06	-
10	Acetylation	GRGKQGGKVRAKAKS CCCCCCCCCCHHCCC	35.06	26051181
10	Lactylation	GRGKQGGKVRAKAKS CCCCCCCCCCHHCCC	35.06	31645732
10	Lactoylation	GRGKQGGKVRAKAKS CCCCCCCCCCHHCCC	35.06	-
17	Phosphorylation	KVRAKAKSRSSRAGL CCCHHCCCCCCCCCC	42.10	23882029
19	Phosphorylation	RAKAKSRSSRAGLQF CHHCCCCCCCCCCCC	31.13	30622161
20	Phosphorylation	AKAKSRSSRAGLQFP HHCCCCCCCCCCCCC	25.66	27966365
21	Methylation	KAKSRSSRAGLQFPV HCCCCCCCCCCCCCH	33.64	-
30	Methylation	GLQFPVGRVHRLLRK CCCCCHHHHHHHHHC	22.69	-
37 (in isoform 2)	Ubiquitination	-	52.02	21890473
37 (in isoform 1)	Ubiquitination	-	52.02	21890473
37	Ubiquitination	RVHRLLRKGNYAERV HHHHHHHCCCHHHHC	52.02	21906983
40	Phosphorylation	RLLRKGNYAERVGAG HHHHCCCHHHHCCCC	20.64	28152594
58	Phosphorylation	YLAAVLEYLTAEILE HHHHHHHHHHHHHHH	12.75	-
77	Phosphorylation	AARDNKKTRIIPRHL HHHCCCCCCEEHHHH	28.80	23882029
82	Methylation	KKTRIIPRHLQLAIR CCCCEEHHHHHHHHC	32.51	-
89	Methylation	RHLQLAIRNDEELNK HHHHHHHCCHHHHHH	38.65	-
96 (in isoform 1)	Ubiquitination	-	58.68	21890473
96 (in isoform 2)	Ubiquitination	-	58.68	21890473
96	Acetylation	RNDEELNKLLGKVTI CCHHHHHHHHCCEEE	58.68	8395049
96	Ubiquitination	RNDEELNKLLGKVTI CCHHHHHHHHCCEEE	58.68	21906983
100 (in isoform 1)	Ubiquitination	-	35.85	21890473
100	Acetylation	ELNKLLGKVTIAQGG HHHHHHCCEEECCCC	35.85	24471261
100	Ubiquitination	ELNKLLGKVTIAQGG HHHHHHCCEEECCCC	35.85	21906983
102	Phosphorylation	NKLLGKVTIAQGGVL HHHHCCEEECCCCCC	17.68	20068231
105	Methylation	LGKVTIAQGGVLPNI HCCEEECCCCCCCCE	45.37	-
119	Acetylation	IQAVLLPKKTESQKT EEEEECCCCCCCCCC	72.76	23236377
119 (in isoform 1)	Ubiquitination	-	72.76	21890473
119	Sumoylation	IQAVLLPKKTESQKT EEEEECCCCCCCCCC	72.76	-
119	Ubiquitination	IQAVLLPKKTESQKT EEEEECCCCCCCCCC	72.76	18781797
119	Sumoylation	IQAVLLPKKTESQKT EEEEECCCCCCCCCC	72.76	-
120	Acetylation	QAVLLPKKTESQKTK EEEECCCCCCCCCCC	56.43	15177633
120	Ubiquitination	QAVLLPKKTESQKTK EEEECCCCCCCCCCC	56.43	21906983
121	Phosphorylation	AVLLPKKTESQKTKS EEECCCCCCCCCCCC	47.17	20068231
123	Phosphorylation	LLPKKTESQKTKSK- ECCCCCCCCCCCCC-	42.06	25849741
125	Ubiquitination	PKKTESQKTKSK--- CCCCCCCCCCCC---	68.17	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
121	T	Phosphorylation	Kinase	DCAF1	Q9Y4B6	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
2	S	Acetylation	Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.	-
2	S	Phosphorylation	Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.	-
2	S	Phosphorylation	Phosphorylation on Ser-2 (H2AS1ph) is enhanced during mitosis.	-
2	S	Phosphorylation	Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription.	-
63	K	ubiquitylation	Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties (By similarity).	-
105	Q	Methylation	Glutamine methylation at Gln-105 (H2AQ104me) by FBL is specifically dedicated to polymerase I.	-
120	K	ubiquitylation	Monoubiquitination of Lys-120 (H2AXK119ub) gives a specific tag for epigenetic transcriptional repression.	-
121	T	Phosphorylation	Phosphorylation at Thr-121 (H2AT120ph) by DCAF1 is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of H2AJ_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of H2AJ_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-96, AND MASS SPECTROMETRY.
PubMed: 19608861