GTF2I_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GTF2I_RAT
• UniProt AC: Q5U2Y1
• Protein Name: General transcription factor II-I
• Gene Name: Gtf2i
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 979
• Subcellular Localization: Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm..
• Protein Description: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation (By similarity)..
• Protein Sequence: MAQVAMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKCTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVAVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVMAADADRPMLSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGAEVEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPTKRPKSSEPPPPPPVPEPTNAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAYPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVVSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNSPQTSTVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKTNSSPSVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPATEEIKETDGSSQIKQEPDPTW

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAQVAMSAL ------CCCHHHCCC	16.74	-
16	Phosphorylation	LPAEDEESSESRMVV CCCCCCCCCHHHHHH	37.04	25575281
17	Phosphorylation	PAEDEESSESRMVVT CCCCCCCCHHHHHHH	42.76	25575281
19	Phosphorylation	EDEESSESRMVVTFL CCCCCCHHHHHHHHH	27.85	25575281
96	Phosphorylation	EMHKMKCTTQANRMS HHHHCCCCCCHHHCC	19.20	23984901
97	Phosphorylation	MHKMKCTTQANRMSV HHHCCCCCCHHHCCC	34.84	23984901
103	Phosphorylation	TTQANRMSVDAVEIE CCCHHHCCCCEEEHH	17.38	27097102
130	Acetylation	CYGKALGKSTVVPVP HHHHHCCCCCEEECC	43.75	-
210	Phosphorylation	DADRPMLSPGGSCGP CCCCCCCCCCCCCCC	18.42	27097102
214	Phosphorylation	PMLSPGGSCGPIKVK CCCCCCCCCCCEEEE	23.09	27097102
222 (in isoform 2)	Phosphorylation	-	50.62	23984901
225 (in isoform 2)	Phosphorylation	-	46.31	23984901
228 (in isoform 2)	Phosphorylation	-	31.62	23298284
233 (in isoform 2)	Phosphorylation	-	21.71	23298284
248	Phosphorylation	EESEDPDYYQYNIQG ECCCCCCCEEEEEEC	9.61	-
298	Phosphorylation	VTIEDDDYSPPTKRP EEEECCCCCCCCCCC	30.03	-
302	Phosphorylation	DDDYSPPTKRPKSSE CCCCCCCCCCCCCCC	42.97	28689409
307	Phosphorylation	PPTKRPKSSEPPPPP CCCCCCCCCCCCCCC	42.49	29779826
308	Phosphorylation	PTKRPKSSEPPPPPP CCCCCCCCCCCCCCC	61.32	27097102
334	Acetylation	VREFNFEKWNARITD EEECCHHHHHHHHHH	41.43	-
379	Phosphorylation	QSHVEDLYVEGLPEG HHHHHHHHCCCCCCC	14.58	-
393	Phosphorylation	GIPFRRPSTYGIPRL CCCCCCCHHCCCCHH	32.23	27097102
394	Phosphorylation	IPFRRPSTYGIPRLE CCCCCCHHCCCCHHH	28.57	28432305
395	Phosphorylation	PFRRPSTYGIPRLER CCCCCHHCCCCHHHH	19.86	28432305
431	Acetylation	REDLQLDKPASGVKE CHHHCCCCCCCCCCH	51.59	22902405
484	Phosphorylation	EAYPNDLYVEGLPEN CCCCCCCEECCCCCC	10.18	-
496	Phosphorylation	PENIPFRSPSWYGIP CCCCCCCCCCCCCCC	24.54	23984901
498	Phosphorylation	NIPFRSPSWYGIPRL CCCCCCCCCCCCCHH	33.89	23984901
500	Phosphorylation	PFRSPSWYGIPRLEK CCCCCCCCCCCHHHH	14.98	23984901
537	Phosphorylation	TEVTQPRTNTPVKED CEECCCCCCCCCCCC	50.56	28432305
539	Phosphorylation	VTQPRTNTPVKEDWN ECCCCCCCCCCCCCH	28.92	27097102
601	Phosphorylation	PEGIPFRSPTWFGIP CCCCCCCCCCCCCCC	27.36	27097102
649	Phosphorylation	INTKALQSPKRPRSP HCHHHCCCCCCCCCC	32.99	22108457
655	Phosphorylation	QSPKRPRSPGSNSKV CCCCCCCCCCCCCCC	35.97	25403869
658	Phosphorylation	KRPRSPGSNSKVPEI CCCCCCCCCCCCCEE	41.12	22673903
660	Phosphorylation	PRSPGSNSKVPEIEV CCCCCCCCCCCEEEE	36.41	25532521
668	Phosphorylation	KVPEIEVTVEGPNNN CCCEEEEEEECCCCC	10.47	23984901
676	Phosphorylation	VEGPNNNSPQTSTVR EECCCCCCCCCCEEE	22.10	27097102
679	Phosphorylation	PNNNSPQTSTVRTPT CCCCCCCCCEEECCC	29.74	27097102
680	Phosphorylation	NNNSPQTSTVRTPTQ CCCCCCCCEEECCCC	21.47	23984901
681	Phosphorylation	NNSPQTSTVRTPTQT CCCCCCCEEECCCCC	19.86	23984901
684	Phosphorylation	PQTSTVRTPTQTNGS CCCCEEECCCCCCCC	26.76	27097102
686	Phosphorylation	TSTVRTPTQTNGSNV CCEEECCCCCCCCCC	47.62	27097102
696	Acetylation	NGSNVPFKPRGREFS CCCCCCCCCCCCEEE	27.95	-
703	Phosphorylation	KPRGREFSFEAWNAK CCCCCEEEHHHHHCH	19.88	23984901
765	Phosphorylation	GVPFRRPSTFGIPRL CCCCCCCHHCCCCHH	34.26	27097102
766	Phosphorylation	VPFRRPSTFGIPRLE CCCCCCHHCCCCHHH	28.67	28432305
799	Phosphorylation	FETAIKESTSSKSPP HHHHHHHCCCCCCCC	28.70	28432305
800	Phosphorylation	ETAIKESTSSKSPPR HHHHHHCCCCCCCCC	37.80	26437020
801	Phosphorylation	TAIKESTSSKSPPRK HHHHHCCCCCCCCCC	45.08	26437020
802	Phosphorylation	AIKESTSSKSPPRKT HHHHCCCCCCCCCCC	36.92	26437020
804	Phosphorylation	KESTSSKSPPRKTNS HHCCCCCCCCCCCCC	41.93	25403869
809	Phosphorylation	SKSPPRKTNSSPSVN CCCCCCCCCCCCCCC	41.43	23984901
811	Phosphorylation	SPPRKTNSSPSVNTT CCCCCCCCCCCCCCC	49.19	23984901
812	Phosphorylation	PPRKTNSSPSVNTTA CCCCCCCCCCCCCCC	24.22	23984901
814	Phosphorylation	RKTNSSPSVNTTASG CCCCCCCCCCCCCCC	29.91	23984901
817	Phosphorylation	NSSPSVNTTASGVED CCCCCCCCCCCCCCC	22.91	23984901
818	Phosphorylation	SSPSVNTTASGVEDL CCCCCCCCCCCCCCC	17.39	27097102
820	Phosphorylation	PSVNTTASGVEDLNI CCCCCCCCCCCCCCE	41.08	23984901
831	Phosphorylation	DLNIIQVTIPDDDNE CCCEEEEECCCCCCH	15.49	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
248	Y	Phosphorylation	Kinase	BTK	-	Uniprot
379	Y	Phosphorylation	Kinase	BTK	-	Uniprot
393	S	Phosphorylation	Kinase	PRKG1	-	Uniprot
484	Y	Phosphorylation	Kinase	BTK	-	Uniprot
765	S	Phosphorylation	Kinase	PRKG1	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GTF2I_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GTF2I_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of GTF2I_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.