GTF2I_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GTF2I_MOUSE
• UniProt AC: Q9ESZ8
• Protein Name: General transcription factor II-I
• Gene Name: Gtf2i
• Organism: Mus musculus (Mouse).
• Sequence Length: 998
• Subcellular Localization: Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm..
• Protein Description: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation..
• Protein Sequence: MAQVVMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVLAAEAERSMLSPSGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGPSETDGVDEKLPLSKALQGSHHSSEGNEGTEVEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPTKRLKSTEPPPPPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVVSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNSSPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPVTEEIKETDGSSQIKQEPDPTW

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAQVVMSAL ------CCCHHHCCC	23.37	-
7	Phosphorylation	-MAQVVMSALPAEDE -CCCHHHCCCCCCCC	18.29	24759943
16	Phosphorylation	LPAEDEESSESRMVV CCCCCCCCCHHHHHH	37.04	25266776
17	Phosphorylation	PAEDEESSESRMVVT CCCCCCCCHHHHHHH	42.76	30635358
19	Phosphorylation	EDEESSESRMVVTFL CCCCCCHHHHHHHHH	27.85	30635358
95	Phosphorylation	AEMHKMKSTTQANRM HHHHHCCCCHHHHCC	32.94	25777480
96	Phosphorylation	EMHKMKSTTQANRMS HHHHCCCCHHHHCCC	19.87	25777480
97	Phosphorylation	MHKMKSTTQANRMSV HHHCCCCHHHHCCCC	32.54	25777480
103	Phosphorylation	TTQANRMSVDAVEIE CHHHHCCCCCEEEHH	17.38	27742792
111	Phosphorylation	VDAVEIETLRKTVED CCEEEHHHHHHHHHH	37.21	30635358
130	Acetylation	CYGKALGKSTVVPVP HHHHHCCCCCEEECC	43.75	23806337
207	Phosphorylation	LAAEAERSMLSPSGS HHHHHHHHHCCCCCC	18.64	29472430
210	Phosphorylation	EAERSMLSPSGSCGP HHHHHHCCCCCCCCC	14.37	22942356
212	Phosphorylation	ERSMLSPSGSCGPIK HHHHCCCCCCCCCEE	39.71	25266776
214	Phosphorylation	SMLSPSGSCGPIKVK HHCCCCCCCCCEEEE	21.90	24759943
219	Ubiquitination	SGSCGPIKVKTEPTE CCCCCCEEEECCCCC	40.59	22790023
248	Phosphorylation	EESEDPDYYQYNIQG ECCCCCCCEEEECCC	9.61	-
258 (in isoform 5)	Phosphorylation	-	54.79	21149613
259 (in isoform 5)	Phosphorylation	-	42.25	26643407
262 (in isoform 5)	Phosphorylation	-	10.79	26643407
277 (in isoform 4)	Phosphorylation	-	34.42	23375375
278 (in isoform 4)	Phosphorylation	-	25.35	25338131
286 (in isoform 4)	Phosphorylation	-	47.76	29514104
300 (in isoform 6)	Phosphorylation	-	53.03	25338131
305 (in isoform 6)	Phosphorylation	-	44.41	29514104
317	Phosphorylation	VTIEDDDYSPPTKRL EEEECCCCCCCCCCC	30.03	-
326	Phosphorylation	PPTKRLKSTEPPPPP CCCCCCCCCCCCCCC	42.46	25619855
327	Phosphorylation	PTKRLKSTEPPPPPP CCCCCCCCCCCCCCC	51.42	25619855
353	Acetylation	VREFNFEKWNARITD CHHCCHHHHHHHHHH	41.43	23806337
359	Phosphorylation	EKWNARITDLRKQVE HHHHHHHHHHHHHHH	24.35	25777480
398	Phosphorylation	QSHVEDLYVEGLPEG HHHHHHHHCCCCCCC	14.58	-
412	Phosphorylation	GIPFRRPSTYGIPRL CCCCCCCHHCCCCHH	32.23	28833060
413	Phosphorylation	IPFRRPSTYGIPRLE CCCCCCHHCCCCHHH	28.57	28833060
441	Phosphorylation	KKHELLNSTREDLQL HHHHHHHCCCHHHCC	28.89	-
450	Acetylation	REDLQLDKPASGVKE CHHHCCCCCCCCCCH	51.59	23806337
503	Phosphorylation	EAHPNDLYVEGLPEN CCCCCCCEECCCCCC	10.18	-
515	Phosphorylation	PENIPFRSPSWYGIP CCCCCCCCCCCCCCC	24.54	26745281
517	Phosphorylation	NIPFRSPSWYGIPRL CCCCCCCCCCCCCHH	33.89	26745281
519	Phosphorylation	PFRSPSWYGIPRLEK CCCCCCCCCCCHHHH	14.98	26745281
547	Phosphorylation	RPELLTHSTTEVTQP CHHHHCCCCCEECCC	31.61	29895711
548	Phosphorylation	PELLTHSTTEVTQPR HHHHCCCCCEECCCC	21.26	29895711
552	Phosphorylation	THSTTEVTQPRTNTP CCCCCEECCCCCCCC	26.99	29895711
556	Phosphorylation	TEVTQPRTNTPVKED CEECCCCCCCCCCCC	50.56	21082442
558	Phosphorylation	VTQPRTNTPVKEDWN ECCCCCCCCCCCCCH	28.92	26824392
668	Phosphorylation	INTKALQSPKRPRSP HCHHHCCCCCCCCCC	32.99	27087446
674	Phosphorylation	QSPKRPRSPGSNSKV CCCCCCCCCCCCCCC	35.97	27087446
677	Phosphorylation	KRPRSPGSNSKVPEI CCCCCCCCCCCCCEE	41.12	23684622
679	Phosphorylation	PRSPGSNSKVPEIEV CCCCCCCCCCCEEEE	36.41	23684622
687	Phosphorylation	KVPEIEVTVEGPNNS CCCEEEEEEECCCCC	10.47	25619855
694	Phosphorylation	TVEGPNNSSPQTSAV EEECCCCCCCCCCEE	50.46	23684622
695	Phosphorylation	VEGPNNSSPQTSAVR EECCCCCCCCCCEEE	24.13	27087446
698	Phosphorylation	PNNSSPQTSAVRTPT CCCCCCCCCEEECCC	23.26	25619855
699	Phosphorylation	NNSSPQTSAVRTPTQ CCCCCCCCEEECCCC	21.37	25619855
703	Phosphorylation	PQTSAVRTPTQTNGS CCCCEEECCCCCCCC	24.88	26060331
705	Phosphorylation	TSAVRTPTQTNGSNV CCEEECCCCCCCCCC	47.62	28066266
707	Phosphorylation	AVRTPTQTNGSNVPF EEECCCCCCCCCCCC	43.98	28066266
715	Acetylation	NGSNVPFKPRGREFS CCCCCCCCCCCCEEE	27.95	23806337
722	Phosphorylation	KPRGREFSFEAWNAK CCCCCEEEHHHHHCH	19.88	23684622
784	Phosphorylation	GVPFRRPSTFGIPRL CCCCCCCHHCCCCHH	34.26	28833060
785	Phosphorylation	VPFRRPSTFGIPRLE CCCCCCHHCCCCHHH	28.67	28833060
818	Phosphorylation	FETAIKESTSSKSPP HHHHHHHCCCCCCCC	28.70	30635358
819	Phosphorylation	ETAIKESTSSKSPPR HHHHHHCCCCCCCCC	37.80	25266776
820	Phosphorylation	TAIKESTSSKSPPRK HHHHHCCCCCCCCCC	45.08	25266776
821	Phosphorylation	AIKESTSSKSPPRKI HHHHCCCCCCCCCCC	36.92	28507225
823	Phosphorylation	KESTSSKSPPRKINS HHCCCCCCCCCCCCC	41.93	26824392
830	Phosphorylation	SPPRKINSSPNVNTT CCCCCCCCCCCCCCC	51.12	25619855
831	Phosphorylation	PPRKINSSPNVNTTA CCCCCCCCCCCCCCC	18.92	27087446
836	Phosphorylation	NSSPNVNTTASGVED CCCCCCCCCCCCCCC	21.32	25619855
837	Phosphorylation	SSPNVNTTASGVEDL CCCCCCCCCCCCCCC	17.39	25619855
839	Phosphorylation	PNVNTTASGVEDLNI CCCCCCCCCCCCCCE	41.08	25619855
879	Ubiquitination	VNDLFSRKFGEAIGM HHHHHHHHHHHHHCC	57.43	22790023
920	Phosphorylation	VSFKAPSYLEISSMR CCCCCCCCEEHHHHH	13.80	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
248	Y	Phosphorylation	Kinase	BTK	P35991	Uniprot
398	Y	Phosphorylation	Kinase	BTK	P35991	Uniprot
412	S	Phosphorylation	Kinase	PRKG1	P0C605	Uniprot
503	Y	Phosphorylation	Kinase	BTK	P35991	Uniprot
784	S	Phosphorylation	Kinase	PRKG1	P0C605	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GTF2I_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GTF2I_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PIAS2_MOUSE	Pias2	physical	20211142

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.