GRK4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GRK4_HUMAN
• UniProt AC: P32298
• Protein Name: G protein-coupled receptor kinase 4
• Gene Name: GRK4
• Organism: Homo sapiens (Human).
• Sequence Length: 578
• Subcellular Localization: Cytoplasm. Cytoplasm, cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongl
• Protein Description: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2..
• Protein Sequence: MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MELENIVA -------CCHHHHHH	13.80	22223895
5	Ubiquitination	---MELENIVANSLL ---CCHHHHHHHHHH	46.81	21890473
6	Ubiquitination	--MELENIVANSLLL --CCHHHHHHHHHHH	1.95	21890473
10	Phosphorylation	LENIVANSLLLKARQ HHHHHHHHHHHHHHC	15.62	-
14	Ubiquitination	VANSLLLKARQGGYG HHHHHHHHHHCCCCC	41.30	-
22	Ubiquitination	ARQGGYGKKSGRSKK HHCCCCCCCCCCCCC	34.97	21890473
23	Ubiquitination	RQGGYGKKSGRSKKW HCCCCCCCCCCCCCH	54.50	21890473
24	Phosphorylation	QGGYGKKSGRSKKWK CCCCCCCCCCCCCHH	42.81	-
48	Phosphorylation	QCSELRHSIEKDYSS HHHHHHHHHHHCHHH	26.47	26437602
90	Phosphorylation	FLDAVAEYEVADDED HHHHHHEEEECCCCC	13.13	22210691
104	Phosphorylation	DRSDCGLSILDRFFN CCCCCCHHHHHHHHC	13.34	24719451
139	Phosphorylation	GLKEENPSKKAFEEC CCCCCCCCHHHHHHH	59.37	29083192
147	Phosphorylation	KKAFEECTRVAHNYL HHHHHHHHHHHHHHH	31.30	29083192
184 (in isoform 3)	Ubiquitination	-	54.98	21890473
184 (in isoform 2)	Ubiquitination	-	54.98	21890473
184	Ubiquitination	LERQPVTKNTFRHYR HHCCCCCCCCEEEEE	54.98	21890473
185 (in isoform 2)	Ubiquitination	-	34.77	21890473
185	Ubiquitination	ERQPVTKNTFRHYRV HCCCCCCCCEEEEEE	34.77	21890473
185 (in isoform 3)	Ubiquitination	-	34.77	21890473
195	Ubiquitination	RHYRVLGKGGFGEVC EEEEECCCCCCCCCC	52.26	-
195	Acetylation	RHYRVLGKGGFGEVC EEEEECCCCCCCCCC	52.26	20167786
211	Ubiquitination	CQVRATGKMYACKKL EEEECCCCCHHHHHH	25.12	-
216 (in isoform 1)	Ubiquitination	-	48.63	21890473
216 (in isoform 4)	Ubiquitination	-	48.63	21890473
216	Ubiquitination	TGKMYACKKLQKKRI CCCCHHHHHHHHHHH	48.63	21890473
217 (in isoform 4)	Ubiquitination	-	56.91	21890473
217 (in isoform 1)	Ubiquitination	-	56.91	21890473
217	Ubiquitination	GKMYACKKLQKKRIK CCCHHHHHHHHHHHH	56.91	21890473
223	Ubiquitination	KKLQKKRIKKRKGEA HHHHHHHHHHHHHHH	9.41	21890473
224	Ubiquitination	KLQKKRIKKRKGEAM HHHHHHHHHHHHHHH	51.03	21890473
227	Ubiquitination	KKRIKKRKGEAMALN HHHHHHHHHHHHHHC	70.72	-
229	Ubiquitination	RIKKRKGEAMALNEK HHHHHHHHHHHHCHH	38.00	21890473
230	Ubiquitination	IKKRKGEAMALNEKR HHHHHHHHHHHCHHH	9.38	21890473
244	Phosphorylation	RILEKVQSRFVVSLA HHHHHHHHHHHHHHH	30.58	-
249	Phosphorylation	VQSRFVVSLAYAYET HHHHHHHHHHHHHHC	11.28	-
255	Ubiquitination	VSLAYAYETKDALCL HHHHHHHHCCCEEEE	41.01	21890473
256	Ubiquitination	SLAYAYETKDALCLV HHHHHHHCCCEEEEE	22.88	21890473
256	Phosphorylation	SLAYAYETKDALCLV HHHHHHHCCCEEEEE	22.88	-
409	Phosphorylation	IKNDTEEYSEKFSED HHCCHHHHHHHHHHH	18.85	-
419	Phosphorylation	KFSEDAKSICRMLLT HHHHHHHHHHHHHHH	28.74	-
426	Phosphorylation	SICRMLLTKNPSKRL HHHHHHHHCCHHHCC	25.47	-
430	Phosphorylation	MLLTKNPSKRLGCRG HHHHCCHHHCCCCCC	39.54	-
485	Phosphorylation	VLDIEQFSVVKGIYL CCCHHHHEEEEEEEE	26.20	-
527	Acetylation	GCFKDINKSESEEAL CCCCCCCCCCCCCCC	56.80	7307297
528	Phosphorylation	CFKDINKSESEEALP CCCCCCCCCCCCCCC	41.70	30622161
530	Phosphorylation	KDINKSESEEALPLD CCCCCCCCCCCCCCC	48.07	30622161

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
10	S	Phosphorylation	Kinase	GRK4	P32298	PSP
139	S	Phosphorylation	Kinase	GRK4	P32298	PSP
244	S	Phosphorylation	Kinase	GRK4	P32298	PSP
249	S	Phosphorylation	Kinase	GRK4	P32298	PSP
256	T	Phosphorylation	Kinase	GRK4	P32298	PSP
419	S	Phosphorylation	Kinase	GRK4	P32298	PSP
426	T	Phosphorylation	Kinase	GRK4	P32298	PSP
430	S	Phosphorylation	Kinase	GRK4	P32298	PSP
485	S	Phosphorylation	Kinase	GRK4	P32298	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GRK4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GRK4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FSHR_HUMAN	FSHR	physical	10379886

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.