GNAI1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GNAI1_RAT
• UniProt AC: P10824
• Protein Name: Guanine nucleotide-binding protein G(i) subunit alpha-1
• Gene Name: Gnai1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 354
• Subcellular Localization: Nucleus. Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cell cortex . Membrane ; Lipid-anchor . Localizes in the centrosomes of interphase a
• Protein Description: Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. [PubMed: 19703466]
• Protein Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGCTLSAED ------CCCCCCHHH	17.08	26253820
3	S-palmitoylation	-----MGCTLSAEDK -----CCCCCCHHHH	3.14	10026207
29	Acetylation	NLREDGEKAAREVKL HHHHHHHHHHHHHHH	53.53	14504719
29	Ubiquitination	NLREDGEKAAREVKL HHHHHHHHHHHHHHH	53.53	-
35	Acetylation	EKAAREVKLLLLGAG HHHHHHHHHHEECCC	28.76	130755
35	Ubiquitination	EKAAREVKLLLLGAG HHHHHHHHHHEECCC	28.76	-
46	Ubiquitination	LGAGESGKSTIVKQM ECCCCCCCHHHHHHH	54.42	-
51	Ubiquitination	SGKSTIVKQMKIIHE CCCHHHHHHHHHHHH	40.60	-
92	Ubiquitination	IRAMGRLKIDFGDAA HHHHCCCCCCCCCHH	38.58	-
197	Acetylation	TFKDLHFKMFDVGGQ EECCEEEEEEECCCC	28.10	130753
206	Phosphorylation	FDVGGQRSERKKWIH EECCCCCCCHHHHHH	33.59	8053895
248	Acetylation	NRMHESMKLFDSICN HHHHHHHHHHHHHHC	56.11	129901
252	Phosphorylation	ESMKLFDSICNNKWF HHHHHHHHHHCCCCC	23.00	27097102
271	Ubiquitination	IILFLNKKDLFEEKI EEEEEEHHHHHHHHH	59.60	-
277	Ubiquitination	KKDLFEEKIKKSPLT HHHHHHHHHHCCCCE	53.26	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GNAI1_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
2	G	Myristoylation	Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation.	26253820
2	G	Palmitoylation	Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation.	26253820
3	C	Palmitoylation	Palmitoylation at Cys-3 varies with membrane lipid composition.	26253820

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GNAI1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of GNAI1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.