dbPTM

GFAP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GFAP_MOUSE
UniProt AC	P03995
Protein Name	Glial fibrillary acidic protein
Gene Name	Gfap
Organism	Mus musculus (Mouse).
Sequence Length	430
Subcellular Localization	Cytoplasm. Associated with intermediate filaments..
Protein Description	GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells..
Protein Sequence	MERRRITSARRSYASETVVRGLGPSRQLGTMPRFSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQDETNLRLEAENNLAAYRQEADEATLARVDLERKVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKDSKQEHKDVVM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MERRRITSARRSYA -CCCHHHHHHHHHHH	17.96	-
8	Phosphorylation	MERRRITSARRSYAS CCCHHHHHHHHHHHH	19.87	22817900
11	Methylation	RRITSARRSYASETV HHHHHHHHHHHHHHH	33.46	24129315
12	Phosphorylation	RITSARRSYASETVV HHHHHHHHHHHHHHH	21.35	25521595
13	Phosphorylation	ITSARRSYASETVVR HHHHHHHHHHHHHHH	16.60	25521595
15	Phosphorylation	SARRSYASETVVRGL HHHHHHHHHHHHHCC	26.10	20415495
17	Phosphorylation	RRSYASETVVRGLGP HHHHHHHHHHHCCCC	23.08	22942356
20	Methylation	YASETVVRGLGPSRQ HHHHHHHHCCCCCCC	30.22	24129315
26	Methylation	VRGLGPSRQLGTMPR HHCCCCCCCCCCCCC	38.59	-
30	Phosphorylation	GPSRQLGTMPRFSLS CCCCCCCCCCCCCCC	32.37	29899451
33	Citrullination	RQLGTMPRFSLSRMT CCCCCCCCCCCCCCC	23.98	-
33	Methylation	RQLGTMPRFSLSRMT CCCCCCCCCCCCCCC	23.98	-
33	Citrullination	RQLGTMPRFSLSRMT CCCCCCCCCCCCCCC	23.98	-
35	Phosphorylation	LGTMPRFSLSRMTPP CCCCCCCCCCCCCCC	27.07	22324799
37	Phosphorylation	TMPRFSLSRMTPPLP CCCCCCCCCCCCCCC	20.86	22324799
40	Phosphorylation	RFSLSRMTPPLPARV CCCCCCCCCCCCCCC	22.20	22817900
79	Phosphorylation	ELNDRFASYIEKVRF HHHHHHHHHHHHHHH	24.75	-
83	Ubiquitination	RFASYIEKVRFLEQQ HHHHHHHHHHHHHHH	29.20	22790023
92	Ubiquitination	RFLEQQNKALAAELN HHHHHHHHHHHHHHH	40.20	22790023
104	Ubiquitination	ELNQLRAKEPTKLAD HHHHHHCCCCCCHHH	58.76	-
107	Phosphorylation	QLRAKEPTKLADVYQ HHHCCCCCCHHHHHH	39.52	20139300
108	Ubiquitination	LRAKEPTKLADVYQA HHCCCCCCHHHHHHH	53.01	22790023
113	Phosphorylation	PTKLADVYQAELREL CCCHHHHHHHHHHHH	11.53	20495213
147	Phosphorylation	NFAQDLGTLRQKLQD HHHHHHHHHHHHHHH	26.51	25521595
186	Ubiquitination	ARVDLERKVESLEEE HHHHHHHHHHHHHHH	40.76	22790023
189	Phosphorylation	DLERKVESLEEEIQF HHHHHHHHHHHHHHH	44.03	29899451
199	Ubiquitination	EEIQFLRKIYEEEVR HHHHHHHHHHHHHHH	52.35	22790023
257	Ubiquitination	TEEWYRSKFADLTDA HHHHHHHHHHHHHHH	35.26	22790023
266	Phosphorylation	ADLTDAASRNAELLR HHHHHHHHHHHHHHH	27.99	-
267	Citrullination	DLTDAASRNAELLRQ HHHHHHHHHHHHHHH	41.47	-
267	Citrullination	DLTDAASRNAELLRQ HHHHHHHHHHHHHHH	41.47	-
291	S-nitrosocysteine	RQLQALTCDLESLRG HHHHHHHCCHHHHCC	6.13	-
291	S-nitrosylation	RQLQALTCDLESLRG HHHHHHHCCHHHHCC	6.13	22178444
299	Phosphorylation	DLESLRGTNESLERQ CHHHHCCCCHHHHHH	29.40	29899451
302	Phosphorylation	SLRGTNESLERQMRE HHCCCCHHHHHHHHH	37.29	27087446
318	Phosphorylation	EERHARESASYQEAL HHHHHHHHHHHHHHH	19.57	29899451
320	Phosphorylation	RHARESASYQEALAR HHHHHHHHHHHHHHH	36.31	22807455
321	Phosphorylation	HARESASYQEALARL HHHHHHHHHHHHHHH	15.30	-
336	Ubiquitination	EEEGQSLKEEMARHL HHHHHHHHHHHHHHH	57.80	22790023
365	Ubiquitination	IEIATYRKLLEGEEN HHHHHHHHHHCCCCC	47.19	27667366
380	Phosphorylation	RITIPVQTFSNLQIR CEEEEEEEECCCEEE	29.30	20415495
382	Phosphorylation	TIPVQTFSNLQIRET EEEEEEECCCEEEEC	39.76	22817900
394	Ubiquitination	RETSLDTKSVSEGHL EECCCCCCCCCCCCC	48.83	22790023
395	Phosphorylation	ETSLDTKSVSEGHLK ECCCCCCCCCCCCCC	32.73	29899451
397	Phosphorylation	SLDTKSVSEGHLKRN CCCCCCCCCCCCCCC	44.97	29899451
403	Citrullination	VSEGHLKRNIVVKTV CCCCCCCCCEEEEEE	44.46	-
403	Citrullination	VSEGHLKRNIVVKTV CCCCCCCCCEEEEEE	44.46	-
408	Ubiquitination	LKRNIVVKTVEMRDG CCCCEEEEEEECCCC	35.64	-
413	Citrullination	VVKTVEMRDGEVIKD EEEEEECCCCCEECC	34.96	-
413	Citrullination	VVKTVEMRDGEVIKD EEEEEECCCCCEECC	34.96	-
419	Ubiquitination	MRDGEVIKDSKQEHK CCCCCEECCCHHHCC	62.21	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
7	T	Phosphorylation	Kinase	AURKB	O70126	Uniprot
7	T	Phosphorylation	Kinase	ROCK1	P70335	Uniprot
12	S	Phosphorylation	Kinase	AURKB	O70126	Uniprot
12	S	Phosphorylation	Kinase	ROCK1	P70335	Uniprot
35	S	Phosphorylation	Kinase	AURKB	O70126	Uniprot
35	S	Phosphorylation	Kinase	ROCK1	P70335	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of GFAP_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GFAP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of GFAP_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GFAP_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-40, AND MASSSPECTROMETRY.	16452087 [Abstract]