GAK_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GAK_MOUSE
• UniProt AC: Q99KY4
• Protein Name: Cyclin-G-associated kinase
• Gene Name: Gak
• Organism: Mus musculus (Mouse).
• Sequence Length: 1305
• Subcellular Localization: Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions (By similarity)..
• Protein Description: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity)..
• Protein Sequence: MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPVERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSLLQSALD ------CCHHHHHHH	49.44	-
2	Phosphorylation	------MSLLQSALD ------CCHHHHHHH	49.44	-
6	Phosphorylation	--MSLLQSALDFLAG --CCHHHHHHHHHHC	30.67	24719451
16	Phosphorylation	DFLAGPGSLGGAAGR HHHHCCCCCCCCCCC	27.29	26824392
73	Phosphorylation	YALKRLLSNEEEKNR HHHHHHHCCHHHHHH	46.95	29176673
87	S-palmitoylation	RAIIQEVCFLKKLSG HHHHHHHHHHHHHHC	3.04	28526873
90	Ubiquitination	IQEVCFLKKLSGHPN HHHHHHHHHHHCCCC	31.27	-
135	Ubiquitination	GQLVEFLKRVECKGP CCHHHHHHHCCCCCC	60.77	-
201	Phosphorylation	SATTISHYPDYSWSA CCCEECCCCCCCCHH	7.33	29514104
204	Phosphorylation	TISHYPDYSWSAQKR EECCCCCCCCHHHHH	13.92	29514104
205	Phosphorylation	ISHYPDYSWSAQKRA ECCCCCCCCHHHHHH	23.18	29514104
350	Glutathionylation	PSRAQPPCGGTVNSS CCCCCCCCCCCCCCC	11.33	24333276
401	Phosphorylation	VIQSVANYAKGDLDI HHHHHHHHHCCCCCH	10.43	29514104
454	Phosphorylation	HYAVYNLSPRIYRAS CEEEEECCHHHHHHH	14.43	-
556	Phosphorylation	SHKRYIEYVCDMVAE HHHHHHHHHHHHHHC	9.23	21183079
683	Acetylation	ATTVKFAKYDLDACD CCEEEEEEECCCCCC	42.29	7923663
759 (in isoform 2)	Phosphorylation	-	24.56	29899451
760 (in isoform 2)	Phosphorylation	-	13.97	29899451
768	Phosphorylation	QYDAEAGSPEAEITE HHCCCCCCCCCEEEC	26.98	-
774	Phosphorylation	GSPEAEITESDSPQS CCCCCEEECCCCCCC	22.42	-
776	Phosphorylation	PEAEITESDSPQSSS CCCEEECCCCCCCCC	34.44	25338131
778	Phosphorylation	AEITESDSPQSSSTD CEEECCCCCCCCCCC	33.83	25338131
781	Phosphorylation	TESDSPQSSSTDTNH ECCCCCCCCCCCCCC	29.64	-
792	Phosphorylation	DTNHFLHTLDWQEEK CCCCCEEECCHHHHC	28.67	-
803	Phosphorylation	QEEKEPETGLDNTSP HHHCCCCCCCCCCCC	54.48	28285833
808	Phosphorylation	PETGLDNTSPKESQS CCCCCCCCCCCHHCC	46.18	25521595
809	Phosphorylation	ETGLDNTSPKESQSV CCCCCCCCCCHHCCE	39.98	25521595
813	Phosphorylation	DNTSPKESQSVLIAD CCCCCCHHCCEEEEC	33.60	25619855
815	Phosphorylation	TSPKESQSVLIADGD CCCCHHCCEEEECCC	28.33	25619855
824	Phosphorylation	LIADGDGSEVSDEEE EEECCCCCCCCCHHH	39.29	25521595
827	Phosphorylation	DGDGSEVSDEEEASF CCCCCCCCCHHHHCC	34.94	25521595
833	Phosphorylation	VSDEEEASFPSEERK CCCHHHHCCCCHHCC	40.75	25619855
836	Phosphorylation	EEEASFPSEERKPGA HHHHCCCCHHCCCCC	50.51	25619855
847	Phosphorylation	KPGAGEDTPRLAAGT CCCCCCCCCCCCCCC	13.15	26160508
938	Phosphorylation	EAPLLLASPVSPLGL CCCEEEECCCCCCCC	26.29	-
1038	Phosphorylation	DLLGGWDTWADTATP CCCCCCCCCCCCCCC	18.95	21189417
1114	Phosphorylation	APAPTQKSNSPWQAN CCCCCCCCCCCCCCC	32.49	24759943
1116	Phosphorylation	APTQKSNSPWQANRP CCCCCCCCCCCCCCC	34.31	26643407
1122	Methylation	NSPWQANRPTAPGTS CCCCCCCCCCCCCCC	32.72	24129315
1128	Phosphorylation	NRPTAPGTSWTPQAK CCCCCCCCCCCCCCC	21.74	24759943
1129	Phosphorylation	RPTAPGTSWTPQAKP CCCCCCCCCCCCCCC	34.56	26643407
1131	Phosphorylation	TAPGTSWTPQAKPAP CCCCCCCCCCCCCCC	12.80	26643407
1149	Phosphorylation	EQLRSHFSVIGAREE HHHHHCCEEEECHHH	13.85	23984901
1163	Phosphorylation	ERGVRVPSFAQKPKV HHCCCCCCHHCCCCC	30.29	-
1171	Phosphorylation	FAQKPKVSENDFEDL HHCCCCCCCCCHHHH	36.49	-
1221	Ubiquitination	LLDWIEGKERNIRAL HHHHHCCCHHHHHHH	40.48	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GAK_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GAK_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GAK_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of GAK_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, AND MASSSPECTROMETRY.
PubMed: 17242355