FOLR1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: FOLR1_HUMAN
• UniProt AC: P15328
• Protein Name: Folate receptor alpha
• Gene Name: FOLR1
• Organism: Homo sapiens (Human).
• Sequence Length: 257
• Subcellular Localization: Cell membrane; Lipid-anchor, GPI-anchor. Secreted . Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Apical cell membrane. Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane.
• Protein Description: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation..
• Protein Sequence: MAQRMTTQLLLLLVWVAVVGEAQTRIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAAAMSGAGPWAAWPFLLSLALMLLWLLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
69	N-linked_Glycosylation	KNACCSTNTSQEAHK HCCCCCCCCCHHHHH	22.80	23934049
97	Ubiquitination	GEMAPACKRHFIQDT CCCCHHHHHHHCCCC	51.52	-
136	Ubiquitination	VLNVPLCKEDCEQWW HCCCCCCHHHHHHHH	65.36	-
161	N-linked_Glycosylation	SNWHKGWNWTSGFNK CCCCCCCCCCCCCCC	40.59	18780401
189	S-palmitoylation	FPTPTVLCNEIWTHS CCCCCEECCCHHCCE	3.59	29575903
201	N-linked_Glycosylation	THSYKVSNYSRGSGR CCEECCCCCCCCCCC	41.61	18780401
201	N-linked_Glycosylation	THSYKVSNYSRGSGR CCEECCCCCCCCCCC	41.61	20068230
234	GPI-anchor	RFYAAAMSGAGPWAA HHHHHHHCCCCHHHH	22.29	7578066

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of FOLR1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of FOLR1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of FOLR1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GSHR_HUMAN	GSR	physical	22939629
PSB2_HUMAN	PSMB2	physical	22939629
IMDH2_HUMAN	IMPDH2	physical	22939629
IRAK3_HUMAN	IRAK3	physical	21988832
CNDH2_HUMAN	NCAPH2	physical	21988832

Drug and Disease Associations

• Kegg Disease
• H01295: Neurodegeneration due to cerebral folate transport deficiency
• OMIM Disease
• 613068: Neurodegeneration due to cerebral folate transport deficiency (NCFTD)
• Kegg Drug
• D09343: Farletuzumab (genetical recombination) (JAN); Farletuzumab (USAN)
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

GPI-anchor

"Computational approach for identification and characterization ofGPI-anchored peptides in proteomics experiments.";
Omaetxebarria M.J., Elortza F., Rodriguez-Suarez E., Aloria K.,Arizmendi J.M., Jensen O.N., Matthiesen R.;
Proteomics 7:1951-1960(2007).
Cited for: GPI-ANCHOR AT SER-234, AND MASS SPECTROMETRY.
PubMed: 17566972

"Modification-specific proteomics of plasma membrane proteins:identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment.";
Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S.,Brodbeck U., Peck S.C., Jensen O.N.;
J. Proteome Res. 5:935-943(2006).
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
PubMed: 16602701

"Proteomic analysis of glycosylphosphatidylinositol-anchored membraneproteins.";
Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C.,Jensen O.N.;
Mol. Cell. Proteomics 2:1261-1270(2003).
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
PubMed: 14517339

"Preferred sites of glycosylphosphatidylinositol modification infolate receptors and constraints in the primary structure of thehydrophobic portion of the signal.";
Yan W., Ratnam M.;
Biochemistry 34:14594-14600(1995).
Cited for: GPI-ANCHOR AT SER-234.
PubMed: 7578066

N-linked Glycosylation

"Identification of N-linked glycoproteins in human milk by hydrophilicinteraction liquid chromatography and mass spectrometry.";
Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.;
Proteomics 8:3833-3847(2008).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-161 AND ASN-201, AND MASSSPECTROMETRY.
PubMed: 18780401