FAS_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: FAS_RAT
• UniProt AC: P12785
• Protein Name: Fatty acid synthase
• Gene Name: Fasn
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 2505
• Subcellular Localization: Cytoplasm. Melanosome.
• Protein Description: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein..
• Protein Sequence: MEEVVIAGMSGKLPESENLQEFWANLIGGVDMVTDDDRRWKAGLYGLPKRSGKLKDLSKFDASFFGVHPKQAHTMDPQLRLLLEVSYEAIVDGGINPASLRGTNTGVWVGVSGSEASEALSRDPETLLGYSMVGCQRAMMANRLSFFFDFKGPSIALDTACSSSLLALQNAYQAIRSGECPAAIVGGINLLLKPNTSVQFMKLGMLSPDGTCRSFDDSGNGYCRAEAVVAVLLTKKSLARRVYATILNAGTNTDGCKEQGVTFPSGEAQEQLIRSLYQPGGVAPESLEYIEAHGTGTKVGDPQELNGITRSLCAFRQSPLLIGSTKSNMGHPEPASGLAALTKVLLSLENGVWAPNLHFHNPNPEIPALLDGRLQVVDRPLPVRGGIVGINSFGFGGANVHVILQPNTQQAPAPAPHAALPHLLHASGRTMEAVQGLLEQGRQHSQDLAFVSMLNDIAATPTAAMPFRGYTVLGVEGHVQEVQQVPASQRPLWFICSGMGTQWRGMGLSLMRLDSFRESILRSDEALKPLGVKVSDLLLSTDEHTFDDIVHSFVSLTAIQIALIDLLTSMGLKPDGIIGHSLGEVACGYADGCLSQREAVLAAYWRGQCIKDANLPAGSMAAVGLSWEECKQRCPPGVVPACHNSEDTVTISGPQAAVNEFVEQLKQEGVFAKEVRTGGLAFHSYFMEGIAPTLLQALKKVIREPRPRSARWLSTSIPEAQWQSSLARTSSAEYNVNNLVSPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGVKPSCTIIPLMKRDHKDNLEFFLTNLGKVHLTGIDINPNALFPPVEFPVPRGTPLISPHIKWDHSQTWDIPVAEDFPNGSSSSSATVYNIDASSESSDHYLVDHCIDGRVLFPGTGYLYLVWKTLARSLSLSLEETPVVFENVTFHQATILPRTGTVPLEVRLLEASHAFEVSDSGNLIVSGKVYQWEDPDSKLFDHPEVPIPAESESVSRLTQGEVYKELRLRGYDYGPHFQGVYEATLEGEQGKLLWKDNWVTFMDTMLQISILGFSKQSLQLPTRVTAIYIDPATHLQKVYMLEGDTQVADVTTSRCLGVTVSGGVYISRLQTTATSRRQQEQLVPTLEKFVFTPHVEPECLSESAILQKELQLCKGLAKALQTKATQQGLKMTVPGLEDLPQHGLPRLLAAACQLQLNGNLQLELGEVLARERLLLPEDPLISGLLNSQALKACIDTALENLSTLKMKVVEVLAGEGHLYSHISALLNTQPMLQLEYTATDRHPQALKDVQTKLQQHDVAQGQWDPSGPAPTNLGALDLVVCNCALATLGDPALALDNMVAALKDGGFLLMHTVLKGHALGETLACLPSEVQPGPSFLSQEEWESLFSRKALHLVGLKKSFYGTALFLCRRLSPQDKPIFLPVEDTSFQWVDSLKSILATSSSQPVWLTAMNCPTSGVVGLVNCLRKEPGGHRIRCILLSNLSSTSHVPKLDPGSSELQKVLESDLVMNVYRDGAWGAFRHFQLEQDKPEEQTAHAFVNVLTRGDLASIRWVSSPLKHMQPPSSSGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWASRDCMLGMEFSGRDKCGRRVMGLVPAEGLATSVLLSPDFLWDVPSSWTLEEAASVPVVYTTAYYSLVVRGRIQHGETVLIHSGSGGVGQAAISIALSLGCRVFTTVGSAEKRAYLQARFPQLDDTSFANSRDTSFEQHVLLHTGGKGVDLVLNSLAEEKLQASVRCLAQHGRFLEIGKFDLSNNHPLGMAIFLKNVTFHGILLDALFEGANDSWREVAELLKAGIRDGVVKPLKCTVFPKAQVEDAFRYMAQGKHIGKVLVQVREEEPEAMLPGAQPTLISAISKTFCPEHKSYIITGGLGGFGLELARWLVLRGAQRLVLTSRSGIRTGYQAKHVREWRRQGIHVLVSTSNVSSLEGARALIAEATKLGPVGGVFNLAMVLRDAMLENQTPELFQDVNKPKYNGTLNLDRATREACPELDYFVAFSSVSCGRGNAGQSNYGFANSTMERICEQRRHDGLPGLAVQWGAIGDVGIILEAMGTNDTVVGGTLPQRISSCMEVLDLFLNQPHAVLSSFVLAEKKAVAHGDGEAQRDLVKAVAHILGIRDLAGINLDSSLADLGLDSLMGVEVRQILEREHDLVLPIREVRQLTLRKLQEMSSKAGSDTELAAPKSKNDTSLKQAQLNLSILLVNPEGPTLTRLNSVQSSERPLFLVHPIEGSITVFHSLAAKLSVPTYGLQCTQAAPLDSIPNLAAYYIDCIKQVQPEGPYRVAGYSFGACVAFEMCSQLQAQQGPAPAHNNLFLFDGSHTYVLAYTQSYRAKLTPGCEAEAEAEAICFFIKQFVDAEHSKVLEALLPLKSLEDRVAAAVDLITRSHQSLDRRDLSFAAVSFYYKLRAADQYKPKAKYHGNVILLRAKTGGTYGEDLGADYNLSQVCDGKVSVHIIEGDHRTLLEGRGLESIINIIHSSLAEPRVSVREG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEEVVIAG -------CCEEEEEC	43.05	-
41	Acetylation	TDDDRRWKAGLYGLP CCCHHHHHHHHCCCC	30.66	22902405
49	Acetylation	AGLYGLPKRSGKLKD HHHCCCCCCCCCCCC	65.32	22902405
59	Acetylation	GKLKDLSKFDASFFG CCCCCHHHCCHHHHC	55.34	22902405
63	Phosphorylation	DLSKFDASFFGVHPK CHHHCCHHHHCCCHH	23.97	27097102
70	Acetylation	SFFGVHPKQAHTMDP HHHCCCHHHCCCCCH	45.28	22902405
207	Phosphorylation	FMKLGMLSPDGTCRS EEECCCCCCCCCCCC	16.44	23984901
211	Phosphorylation	GMLSPDGTCRSFDDS CCCCCCCCCCCCCCC	16.48	25575281
214	Phosphorylation	SPDGTCRSFDDSGNG CCCCCCCCCCCCCCC	34.86	27097102
286	Phosphorylation	PGGVAPESLEYIEAH CCCCCHHHHHHHHHH	25.99	25575281
289	Phosphorylation	VAPESLEYIEAHGTG CCHHHHHHHHHHCCC	14.89	25575281
295	Phosphorylation	EYIEAHGTGTKVGDP HHHHHHCCCCCCCCH	31.63	25575281
297	Phosphorylation	IEAHGTGTKVGDPQE HHHHCCCCCCCCHHH	23.53	25575281
298	Acetylation	EAHGTGTKVGDPQEL HHHCCCCCCCCHHHH	45.63	25786129
311	Phosphorylation	ELNGITRSLCAFRQS HHCCCCHHHHHHHCC	21.10	25575281
318	Phosphorylation	SLCAFRQSPLLIGST HHHHHHCCCEEEECC	17.17	28432305
324	Phosphorylation	QSPLLIGSTKSNMGH CCCEEEECCCCCCCC	26.18	25575281
325	Phosphorylation	SPLLIGSTKSNMGHP CCEEEECCCCCCCCC	33.02	25575281
528	Succinylation	LRSDEALKPLGVKVS HCCCHHHHHHCCCHH	45.29	26843850
528	Acetylation	LRSDEALKPLGVKVS HCCCHHHHHHCCCHH	45.29	22902405
673	Acetylation	KQEGVFAKEVRTGGL HHCCCCEEECCCCCC	45.32	25786129
724	Phosphorylation	IPEAQWQSSLARTSS CCHHHHHHHHHCCCC	25.10	27097102
725	Phosphorylation	PEAQWQSSLARTSSA CHHHHHHHHHCCCCC	15.79	27097102
786	Acetylation	CTIIPLMKRDHKDNL CEEEECCCCCCCCCH	63.46	22902405
790	Acetylation	PLMKRDHKDNLEFFL ECCCCCCCCCHHHHH	53.23	22902405
902	Phosphorylation	VWKTLARSLSLSLEE HHHHHHHHCCCCCCC	19.39	23984901
904	Phosphorylation	KTLARSLSLSLEETP HHHHHHCCCCCCCCC	19.70	23984901
906	Phosphorylation	LARSLSLSLEETPVV HHHHCCCCCCCCCEE	30.29	23984901
967	Acetylation	QWEDPDSKLFDHPEV EEECCCCCCCCCCCC	61.63	22902405
980	Phosphorylation	EVPIPAESESVSRLT CCCCCCCCCHHCCCC	36.96	27097102
982	Phosphorylation	PIPAESESVSRLTQG CCCCCCCHHCCCCCC	34.37	27097102
984	Phosphorylation	PAESESVSRLTQGEV CCCCCHHCCCCCCCH	30.74	27097102
993	Acetylation	LTQGEVYKELRLRGY CCCCCHHHHHHHCCC	56.29	22902405
1130	Phosphorylation	HVEPECLSESAILQK CCCHHHCCHHHHHHH	40.96	23984901
1132	Phosphorylation	EPECLSESAILQKEL CHHHCCHHHHHHHHH	19.75	23984901
1234	Acetylation	LENLSTLKMKVVEVL HHHHHHHHEEHHHHH	36.44	22902405
1276	Acetylation	DRHPQALKDVQTKLQ CCCHHHHHHHHHHHH	59.89	22902405
1386	Acetylation	ALHLVGLKKSFYGTA HHHHHCCCHHHHHHH	40.20	22902405
1405	Acetylation	RRLSPQDKPIFLPVE HCCCCCCCCCEEECC	34.46	22902405
1464	S-nitrosylation	PGGHRIRCILLSNLS CCCCEEEEEEEECCC	2.09	-
1483	Phosphorylation	VPKLDPGSSELQKVL CCCCCCCCHHHHHHH	26.48	22673903
1484	Phosphorylation	PKLDPGSSELQKVLE CCCCCCCHHHHHHHH	48.52	22673903
1488	Acetylation	PGSSELQKVLESDLV CCCHHHHHHHHCCCE	62.50	22902405
1516	Acetylation	HFQLEQDKPEEQTAH CEECCCCCCHHHHHH	54.76	22902405
1574	Phosphorylation	FRDIMLATGKLSPDA HHHHHHHCCCCCCCC	30.78	28432305
1576	Acetylation	DIMLATGKLSPDAIP HHHHHCCCCCCCCCC	41.24	25786129
1578	Phosphorylation	MLATGKLSPDAIPGK HHHCCCCCCCCCCCC	24.99	30240740
1585	Acetylation	SPDAIPGKWASRDCM CCCCCCCCCCCCCCC	33.94	22902405
1588	Phosphorylation	AIPGKWASRDCMLGM CCCCCCCCCCCCCCC	27.77	-
1698	N6-(pyridoxal phosphate)lysine	TTVGSAEKRAYLQAR EECCCHHHHHHHHHH	41.88	-
1698	Other	TTVGSAEKRAYLQAR EECCCHHHHHHHHHH	41.88	-
1698	Acetylation	TTVGSAEKRAYLQAR EECCCHHHHHHHHHH	41.88	25786129
1720	Phosphorylation	SFANSRDTSFEQHVL CCCCCCCCCHHHEEE	33.92	27097102
1721	Phosphorylation	FANSRDTSFEQHVLL CCCCCCCCHHHEEEE	30.95	27097102
1746	Acetylation	LNSLAEEKLQASVRC HHHHHHHHHHHHHHH	37.09	25786129
1765	Acetylation	GRFLEIGKFDLSNNH CCEEEEEEEECCCCC	41.17	22902405
1818	Acetylation	GIRDGVVKPLKCTVF CCCCCCCCCCCCEEC	42.07	22902405
1841	Acetylation	FRYMAQGKHIGKVLV HHHHHCCCCCEEEEE	21.70	22902405
1909	Phosphorylation	GAQRLVLTSRSGIRT CCCEEEEEECCCCCC	18.02	22673903
1910	Phosphorylation	AQRLVLTSRSGIRTG CCEEEEEECCCCCCC	21.93	22673903
1921	Acetylation	IRTGYQAKHVREWRR CCCCCHHHHHHHHHH	27.40	22902405
1987	Acetylation	ELFQDVNKPKYNGTL HHHHCCCCCCCCCCC	42.61	22902405
1989	Acetylation	FQDVNKPKYNGTLNL HHCCCCCCCCCCCCC	53.62	22902405
1993	Phosphorylation	NKPKYNGTLNLDRAT CCCCCCCCCCCCHHH	14.61	23984901
2085	S-nitrosylation	LPQRISSCMEVLDLF HHHHHHHHHHHHHHH	1.84	-
2124	Acetylation	EAQRDLVKAVAHILG HHHHHHHHHHHHHHC	44.43	22902405
2142	Phosphorylation	LAGINLDSSLADLGL HCCCCCCHHHHHCCH	30.34	22673903
2143	Phosphorylation	AGINLDSSLADLGLD CCCCCCHHHHHCCHH	27.52	22673903
2151	O-(pantetheine 4'-phosphoryl)serine	LADLGLDSLMGVEVR HHHCCHHHHCCHHHH	25.87	-
2151	Phosphorylation	LADLGLDSLMGVEVR HHHCCHHHHCCHHHH	25.87	22673903
2186	Phosphorylation	LRKLQEMSSKAGSDT HHHHHHHHHHCCCCC	28.12	23984901
2187	Phosphorylation	RKLQEMSSKAGSDTE HHHHHHHHHCCCCCH	25.80	23984901
2188	Ubiquitination	KLQEMSSKAGSDTEL HHHHHHHHCCCCCHH	49.84	-
2191	Phosphorylation	EMSSKAGSDTELAAP HHHHHCCCCCHHCCC	46.34	23991683
2193	Phosphorylation	SSKAGSDTELAAPKS HHHCCCCCHHCCCCC	34.30	28689409
2230	Phosphorylation	PTLTRLNSVQSSERP CCCCCCCCCCCCCCC	26.56	23984901
2233	Phosphorylation	TRLNSVQSSERPLFL CCCCCCCCCCCCEEE	31.82	23984901
2234	Phosphorylation	RLNSVQSSERPLFLV CCCCCCCCCCCEEEE	22.12	23984901
2247	Phosphorylation	LVHPIEGSITVFHSL EEEECCCCEEHHHHH	11.42	23984901
2249	Phosphorylation	HPIEGSITVFHSLAA EECCCCEEHHHHHHH	20.86	23984901
2253	Phosphorylation	GSITVFHSLAAKLSV CCEEHHHHHHHHCCC	14.29	28689409
2375	Phosphorylation	QFVDAEHSKVLEALL HHCCHHHHHHHHHHH	18.90	25575281
2376	Acetylation	FVDAEHSKVLEALLP HCCHHHHHHHHHHHC	53.39	22902405
2385	Acetylation	LEALLPLKSLEDRVA HHHHHCCCCHHHHHH	51.56	22902405
2404	Phosphorylation	LITRSHQSLDRRDLS HHHHCCCCCCCCCCH	27.15	29779826
2428	Acetylation	LRAADQYKPKAKYHG HHHHHHCCCCCEECC	34.12	22902405
2432	Acetylation	DQYKPKAKYHGNVIL HHCCCCCEECCEEEE	43.86	22902405
2501	Phosphorylation	SLAEPRVSVREG--- HCCCCCCCCCCC---	19.41	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of FAS_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1464	C	S-nitrosylation	S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1464 or Cys-2085 is important for the enzyme dimerization.	-
2085	C	S-nitrosylation	S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1464 or Cys-2085 is important for the enzyme dimerization.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of FAS_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of FAS_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.