ERCC3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ERCC3_MOUSE
• UniProt AC: P49135
• Protein Name: General transcription and DNA repair factor IIH helicase subunit XPB
• Gene Name: Ercc3
• Organism: Mus musculus (Mouse).
• Sequence Length: 783
• Subcellular Localization: Nucleus.
• Protein Description: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription..
• Protein Sequence: MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELITETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKASSKHVHPLFKRFRK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
19	Phosphorylation	KKSKKRQYEEEEEDE HHHHHHHHHHHCCCC	29.37	26643407
34	Phosphorylation	DDIPGNESQEAVPSA CCCCCCCCCCCCCCC	37.00	27087446
40	Phosphorylation	ESQEAVPSAAGKQVD CCCCCCCCCCCCCCC	24.93	23984901
223	Phosphorylation	SKSAISKTAAEGSGG CCHHHCCCCCCCCCC	25.06	25293948
228	Phosphorylation	SKTAAEGSGGPSTSQ CCCCCCCCCCCCHHC	31.89	25293948
232	Phosphorylation	AEGSGGPSTSQGVDA CCCCCCCCHHCCCCC	43.92	25293948
233	Phosphorylation	EGSGGPSTSQGVDAQ CCCCCCCHHCCCCCC	28.36	25293948
234	Phosphorylation	GSGGPSTSQGVDAQA CCCCCCHHCCCCCCC	29.31	25293948
357	Phosphorylation	VGVTAACTVRKRCLV HHHHHHEEEHHHEEE	20.72	28059163
582	Phosphorylation	RLNKPYIYGPTSQGE HCCCCEEECCCCHHH	15.94	22817900
602	Ubiquitination	QNFKHNPKINTIFIS HHHCCCCCCCEEEEE	54.64	22790023
677	Dimethylation	EMAYSTKRQRFLVDQ HHHHHCCHHHHEECC	32.75	-
677	Methylation	EMAYSTKRQRFLVDQ HHHHHCCHHHHEECC	32.75	18964951
687	Phosphorylation	FLVDQGYSFKVITKL HEECCCCCEEEHHHH	25.97	-
692	Phosphorylation	GYSFKVITKLAGMEE CCCEEEHHHHCCCCH	24.00	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ERCC3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ERCC3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ERCC3_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of ERCC3_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.