EPAS1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: EPAS1_RAT
• UniProt AC: Q9JHS1
• Protein Name: Endothelial PAS domain-containing protein 1
• Gene Name: Epas1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 874
• Subcellular Localization: Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles.
• Protein Description: Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity)..
• Protein Sequence: MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKTGSGFGKKNKDRSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELVGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVVYNPRNLQPQCIMCVNYVLSEIEKNDVVVSMDQTESCSSHTLMAMNSIFDTSDDVALSEKSNYLFTNLKEEPEDLAQLAPTPGDAIISLDFGSQNFDESSTYGKAILPPGQPWATELRSHSAQSESRSLPAFTVPQAGSPGNATPSATSSSSCSTPSSPEDYYSSLENHLKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEEPLVPESPQPNPQHCFSTMSSIFQPLTPGASQGTFFLDKYPQQLESRKTESEHWPMSTIFFDAGSKGSLPPCCGQASTPLSSMGGRSNTPWPPDPPLHLGPTKWSVGNQSAEPLGPLPLGTSQLEPPSTPPHVSMFKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLDYEEPAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTVSASMAPDEFTQKSMRGLGQPLRHLPPSQPPSTRSPGENAKSGFPPQCYASPFQDYSPPGAQKGSGVASRLLGPSFEPYLLPELTRYDCEVNVPEPGSSTLLQGRDLLRALDQAT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
405	Hydroxylation	EDLAQLAPTPGDAII HHHHHHCCCCCCEEE	47.95	-
530	Hydroxylation	LDLETLAPYIPMDGE CCHHHHCCCCCCCCC	30.60	-
844	Phosphorylation	PYLLPELTRYDCEVN CCCCHHHCCCEEEEC	26.06	-
851	Hydroxylation	TRYDCEVNVPEPGSS CCCEEEECCCCCCCC	22.72	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of EPAS1_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
405	P	Hydroxylation	In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3.	-
530	P	Hydroxylation	In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3.	-
851	N	Hydroxylation	In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of EPAS1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of EPAS1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.