dbPTM

EI2BE_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EI2BE_RAT
UniProt AC	Q64350
Protein Name	Translation initiation factor eIF-2B subunit epsilon
Gene Name	Eif2b5
Organism	Rattus norvegicus (Rat).
Sequence Length	716
Subcellular Localization
Protein Description	Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP..
Protein Sequence	MAATAAVPSAVGGRANKRGGGSGGGGTQGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLQKSKWCHPTSLNVVRIITSDLYRSLGDVLRDVDAKALVRSDFLLIYGDVVSNINISKALEEHRLRRKLEKNVSVMTMVFKESSPSHPTRCHEDNVVLAVDSTTNRILHFQKTQGLRHFSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNFTDSSTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGAGTVVGSNCSITNSVIGPNCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVILKPHCVLTSQVVVGPDIILPEGSVISLHPPDAEEDEDDGQFSDDSGADQEKEKVKLKGYNPAEVGPEGQGYLWKAEDVDEKEDEELRQSLWGLMINMEEESETESERSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGREENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHETLVPSLAKVLMAFYQLEILAEETILSWFSQRDITDKGQQLRKNQQLQRFIQWLREAEEESSDDD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Methylation	VGGRANKRGGGSGGG HCCCCCCCCCCCCCC	49.12	-
22	Phosphorylation	ANKRGGGSGGGGTQG CCCCCCCCCCCCCCC	37.22	23707720
27	Phosphorylation	GGSGGGGTQGAEEEP CCCCCCCCCCCCCCC	27.40	25575281
56	Ubiquitination	RRFFPISKDQPRVLL CCCCCCCCCCCCEEH	62.19	23707720
98	Ubiquitination	CWKAAQIKEHLQKSK HHHHHHHHHHHHHCC	27.31	23707720
125	Phosphorylation	ITSDLYRSLGDVLRD HHHHHHHHHHHHHHH	24.13	23707720
136	Ubiquitination	VLRDVDAKALVRSDF HHHHCCHHHHHCCCE	37.48	23707720
212	Ubiquitination	NRILHFQKTQGLRHF CCEEEEECCCCCCCE	41.70	23707720
317	Phosphorylation	RRWVYPLTPEVNFTD HHHHCCCCCCCCCCC	16.95	23707720
445	Phosphorylation	LPEGSVISLHPPDAE CCCCCEEEECCCCCC	20.61	-
461	Phosphorylation	DEDDGQFSDDSGADQ CCCCCCCCCCCCCCH	32.40	11500362
464	Phosphorylation	DGQFSDDSGADQEKE CCCCCCCCCCCHHHH	40.65	11500362
500	Ubiquitination	KAEDVDEKEDEELRQ ECCCCCHHHCHHHHH	67.03	23707720
527	Phosphorylation	SETESERSVDPEELD CCCCCCCCCCHHHHH	27.16	22673903
535	Phosphorylation	VDPEELDSRAGSPQL CCHHHHHHCCCCCCC	36.33	11500362
539	Phosphorylation	ELDSRAGSPQLDDIR HHHHCCCCCCCCHHH	14.42	11311121
712	Phosphorylation	LREAEEESSDDD--- HHHHHHHCCCCC---	42.20	11500362
713	Phosphorylation	REAEEESSDDD---- HHHHHHCCCCC----	48.62	11500362

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
464	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
535	S	Phosphorylation	Kinase	GSK3A	P18265	PSP
535	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
535	S	Phosphorylation	Kinase	GSK3B	P18266	PSP
535	S	Phosphorylation	Kinase	GSK-FAMILY	-	GPS
535	S	Phosphorylation	Kinase	GSK-3_GROUP	-	PhosphoELM
539	S	Phosphorylation	Kinase	DYRK1A	Q13627	PSP
539	S	Phosphorylation	Kinase	DYRK2	Q92630	PSP
539	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
539	S	Phosphorylation	Kinase	DYRK2	-	Uniprot
712	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
712	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
712	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
713	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
713	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
713	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
539	S	Phosphorylation		11311121
Phosphorylated at Ser-539 by DYRK2; this is required for subsequent phosphorylation by GSK3B.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EI2BE_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
UBC_HUMAN	UBC	physical	23707720
NEDD4_MOUSE	Nedd4	physical	23707720

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EI2BE_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."; Moser K., White F.M.; J. Proteome Res. 5:98-104(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-539, AND MASSSPECTROMETRY.	16396499 [Abstract]