dbPTM

EHD4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EHD4_MOUSE
UniProt AC	Q9EQP2
Protein Name	EH domain-containing protein 4 {ECO:0000305}
Gene Name	Ehd4 {ECO:0000312\|MGI:MGI:1919619}
Organism	Mus musculus (Mouse).
Sequence Length	541
Subcellular Localization	Early endosome membrane Peripheral membrane protein Cytoplasmic side . Recycling endosome membrane Peripheral membrane protein Cytoplasmic side . Cell membrane Peripheral membrane protein Cytoplasmic side .
Protein Description	ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport..
Protein Sequence	MFSWMGRQAGGRERSGGMDAVQTVTGGLRSLYQRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISIIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPNMFGKENKKRELIYRLPEIYVQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLTNKISSLMGLISQEEMNMPTQMVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPNSLPPHLVPPSHRKSLPKAD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MFSWMGRQ -------CCCCCCCC	5.61	-
15	Phosphorylation	QAGGRERSGGMDAVQ CCCCCCCCCCCCHHH	34.64	27742792
35	Phosphoglycerylation	LRSLYQRKVLPLEEA HHHHHHCCCCCHHHH	32.38	-
35	Ubiquitination	LRSLYQRKVLPLEEA HHHHHHCCCCCHHHH	32.38	-
61	Ubiquitination	EDADFENKPMILLVG CCCCCCCCCEEEEEE	28.85	-
70	Phosphorylation	MILLVGQYSTGKTTF EEEEEEECCCCHHHH	11.41	-
129	Phosphorylation	KKPFRKLSRFGNAFL CCCCHHHHHHHHHHH	28.71	24719451
141	S-nitrosylation	AFLNRFMCSQLPNQV HHHHHHHHHCCCHHH	1.84	20925432
141	S-nitrosocysteine	AFLNRFMCSQLPNQV HHHHHHHHHCCCHHH	1.84	-
150	Ubiquitination	QLPNQVLKSISIIDS CCCHHHHHHCEEECC	47.06	-
151	Phosphorylation	LPNQVLKSISIIDSP CCHHHHHHCEEECCC	19.87	20469934
162	Phosphorylation	IDSPGILSGEKQRIS ECCCCCCCCCHHHHH	42.88	27742792
165	Ubiquitination	PGILSGEKQRISRGY CCCCCCCHHHHHCCC	48.69	-
223	Ubiquitination	KIRVVLNKADQVDTQ EEEEEEECHHCCCHH	49.53	-
291	Ubiquitination	DIQSLPQKAAVRKLN HHHHCCHHHHHHHHH	36.05	-
296	Ubiquitination	PQKAAVRKLNDLIKR CHHHHHHHHHHHHHH	45.07	-
302	Ubiquitination	RKLNDLIKRARLAKV HHHHHHHHHHHHHHH	47.50	-
302	Acetylation	RKLNDLIKRARLAKV HHHHHHHHHHHHHHH	47.50	7614619
349	Phosphorylation	YVQLQREYQISAGDF HHHHHHHHCCCCCCC	17.21	22871156
360	Ubiquitination	AGDFPEVKAMQEQLE CCCCHHHHHHHHHHH	36.16	-
369	Phosphorylation	MQEQLENYDFTKFHS HHHHHHCCCCHHHHC	11.76	22871156
372	Phosphorylation	QLENYDFTKFHSLKP HHHCCCCHHHHCCCH	29.87	24719451
376	Phosphorylation	YDFTKFHSLKPKLIE CCCHHHHCCCHHHHH	41.02	26824392
451	Phosphorylation	VAKDKPVYDELFYTL EECCCCCCEECEEEC	16.54	25367039
456	Phosphorylation	PVYDELFYTLSPING CCCEECEEECCCCCC	21.28	-
457	Phosphorylation	VYDELFYTLSPINGK CCEECEEECCCCCCE	17.27	21743459
459	Phosphorylation	DELFYTLSPINGKIS EECEEECCCCCCEEC	18.88	21082442
478	Ubiquitination	KKEMVTSKLPNSVLG CHHHHHCCCCCCHHH	59.61	-
482	Phosphorylation	VTSKLPNSVLGKIWK HHCCCCCCHHHHHHH	19.44	22942356
486	Ubiquitination	LPNSVLGKIWKLADC CCCCHHHHHHHHHCC	41.03	-
518	Phosphorylation	IKIKLDGYELPNSLP HEEEECCCCCCCCCC	17.60	29514104
536	Phosphorylation	VPPSHRKSLPKAD-- CCHHHHCCCCCCC--	50.27	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of EHD4_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EHD4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EHD4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of EHD4_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EHD4_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, AND MASSSPECTROMETRY.	19367708 [Abstract]