EAA2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: EAA2_RAT
• UniProt AC: P31596
• Protein Name: Excitatory amino acid transporter 2
• Gene Name: Slc1a2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 573
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein .
• Protein Description: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. [PubMed: 1448170]
• Protein Sequence: MASTEGANNMPKQVEVRMHDSHLSSEEPKHRNLGMRMCDKLGKNLLLSLTVFGVILGAVCGGLLRLAAPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPSEEANTTKAVISLLNETMNEAPEETKIVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGEQAKLMVEFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVILDGGQIVTVSLTATLASIGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRMHEDIEMTKTQSVYDDTKNHRESNSNQCVYAAHNSVVIDECKVTLAANGKSADCSVEEEPWKREK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MASTEGANNM -----CCCCCCCCCC	26.20	22673903
4	Phosphorylation	----MASTEGANNMP ----CCCCCCCCCCC	28.97	22673903
12	Ubiquitination	EGANNMPKQVEVRMH CCCCCCCCEEEEEEC	57.09	-
21	Phosphorylation	VEVRMHDSHLSSEEP EEEEECCCCCCCCCH	16.20	25403869
24	Phosphorylation	RMHDSHLSSEEPKHR EECCCCCCCCCHHHC	30.22	30240740
25	Phosphorylation	MHDSHLSSEEPKHRN ECCCCCCCCCHHHCC	52.95	25403869
29	Acetylation	HLSSEEPKHRNLGMR CCCCCCHHHCCHHHH	59.48	22902405
29	Ubiquitination	HLSSEEPKHRNLGMR CCCCCCHHHCCHHHH	59.48	-
38	S-palmitoylation	RNLGMRMCDKLGKNL CCHHHHHHHHHHHHH	2.70	20685337
40	Ubiquitination	LGMRMCDKLGKNLLL HHHHHHHHHHHHHHH	54.96	-
43	Ubiquitination	RMCDKLGKNLLLSLT HHHHHHHHHHHHHHH	55.66	-
113	Phosphorylation	SGLDAKASGRLGTRA CCCCHHHCCCCCHHH	24.60	7903307
205	N-linked_Glycosylation	APPSEEANTTKAVIS CCCCHHHCHHHHHHH	52.11	-
215	N-linked_Glycosylation	KAVISLLNETMNEAP HHHHHHHHHHHHCCC	48.24	-
382	Acetylation	EDNLGIDKRVTRFVL HCCCCCCCCEEEEEE	47.01	22902405
403	Phosphorylation	NMDGTALYEAVAAIF CCCCHHHHHHHHHHH	10.20	12384259
479	Phosphorylation	LLDRMRTSVNVVGDS HHHHHCCCCEECCCC	11.00	12324450
486	Phosphorylation	SVNVVGDSFGAGIVY CCEECCCCCCCCHHE	21.46	12324450
493	Phosphorylation	SFGAGIVYHLSKSEL CCCCCHHEEECHHHH	8.63	-
496	Phosphorylation	AGIVYHLSKSELDTI CCHHEEECHHHHCCH	22.33	12324450
497	Acetylation	GIVYHLSKSELDTID CHHEEECHHHHCCHH	55.07	22902405
497	Ubiquitination	GIVYHLSKSELDTID CHHEEECHHHHCCHH	55.07	-
498	Phosphorylation	IVYHLSKSELDTIDS HHEEECHHHHCCHHH	39.76	12324450
502	Phosphorylation	LSKSELDTIDSQHRM ECHHHHCCHHHHCCC	39.03	22673903
505	Phosphorylation	SELDTIDSQHRMHED HHHCCHHHHCCCHHH	24.84	12324450
517	Ubiquitination	HEDIEMTKTQSVYDD HHHHHCCCCCCCCCC	43.17	-
518	Phosphorylation	EDIEMTKTQSVYDDT HHHHCCCCCCCCCCC	19.53	23984901
520	Phosphorylation	IEMTKTQSVYDDTKN HHCCCCCCCCCCCCC	28.07	22505712
522	Phosphorylation	MTKTQSVYDDTKNHR CCCCCCCCCCCCCCC	17.22	-
526	Ubiquitination	QSVYDDTKNHRESNS CCCCCCCCCCCCCCC	58.72	-
531	Phosphorylation	DTKNHRESNSNQCVY CCCCCCCCCCCCCEE	45.58	27097102
533	Phosphorylation	KNHRESNSNQCVYAA CCCCCCCCCCCEEEE	37.24	30411139
538	Phosphorylation	SNSNQCVYAAHNSVV CCCCCCEEEECCEEE	13.00	27097102
543	Phosphorylation	CVYAAHNSVVIDECK CEEEECCEEEEEECE	14.11	-
550	Ubiquitination	SVVIDECKVTLAANG EEEEEECEEEEEECC	35.89	-
558	Ubiquitination	VTLAANGKSADCSVE EEEEECCCCCCCCCC	42.13	-
559	Phosphorylation	TLAANGKSADCSVEE EEEECCCCCCCCCCC	30.64	-
562	S-nitrosocysteine	ANGKSADCSVEEEPW ECCCCCCCCCCCCCC	4.99	-
562	S-nitrosylation	ANGKSADCSVEEEPW ECCCCCCCCCCCCCC	4.99	22178444
563	Phosphorylation	NGKSADCSVEEEPWK CCCCCCCCCCCCCCC	32.86	28551015
570	Acetylation	SVEEEPWKREK---- CCCCCCCCCCC----	62.49	22902405
570	Ubiquitination	SVEEEPWKREK---- CCCCCCCCCCC----	62.49	-
570	Sumoylation	SVEEEPWKREK---- CCCCCCCCCCC----	62.49	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of EAA2_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
38	C	Palmitoylation	Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of EAA2_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of EAA2_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.