dbPTM

DNJC2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DNJC2_MOUSE
UniProt AC	P54103
Protein Name	DnaJ homolog subfamily C member 2
Gene Name	Dnajc2
Organism	Mus musculus (Mouse).
Sequence Length	621
Subcellular Localization	Nucleus . Cytoplasm, cytosol .
Protein Description	Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation (By similarity). Specifically binds DNA sequence 5'-GTCAAGC-3'..
Protein Sequence	MLLLPSAAEGQGTAITHALTSASSVCQVEPVGRWFEAFVKRRNRNASTSFQELEDKKELSEESEDEELQLEEFPMLKTLDPKDWKNQDHYAVLGLGHVRYTATQRQIKAAHKAMVLKHHPDKRKAAGEPIKEGDNDYFTCITKAYEMLSDPVKRRAFNSVDPTFDNSVPSKSEAKDNFFQVFSPVFERNSRWSNKKNVPKLGDMNSSFEDVDAFYSFWYNFDSWREFSYLDEEEKEKAECRDERKWIEKQNRATRAQRKKEEMNRIRTLVDNAYSCDPRIKKFKEEEKAKKEAEKKAKAEARRKEQEAKEKQRQAELEAVRLAKEKEEEEVRQQALLAKKEKDIQKKAIKKERQKLRNSCKSWNHFSDNEADRVKMMEEVEKLCDRLELASLQGLNEILASSTREVGKAALEKQIEEVNEQMRREKEEADARMRQASKNAEKSTGGSGSGSKNWSEDDLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVISKAKSLQKLDPHQKDDINKKAFDKFKKEHGVASQADSAAPSERFEGPCIDSTPWTTEEQKLLEQALKTYPVNTPERWEKIAEAVPGRTKKDCMRRYKELVEMVKAKKAAQEQVLNASRARK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MLLLPSAA -------CCCCCCCC	5.55	-
47	Phosphorylation	KRRNRNASTSFQELE HHHCCCCCCCHHHHH	28.29	27087446
48	Phosphorylation	RRNRNASTSFQELED HHCCCCCCCHHHHHH	31.02	25521595
49	Phosphorylation	RNRNASTSFQELEDK HCCCCCCCHHHHHHH	23.57	25521595
60	Phosphorylation	LEDKKELSEESEDEE HHHHHHHCCCCCCCC	40.17	24925903
63	Phosphorylation	KKELSEESEDEELQL HHHHCCCCCCCCCCH	46.42	27087446
90	Phosphorylation	DWKNQDHYAVLGLGH HHCCCCCEEEEECCC	13.49	29514104
137	Phosphorylation	IKEGDNDYFTCITKA CCCCCCCHHHHHHHH	13.67	20531401
139	Phosphorylation	EGDNDYFTCITKAYE CCCCCHHHHHHHHHH	9.21	20531401
183	Phosphorylation	DNFFQVFSPVFERNS CCCHHHCHHHHHHCC	22.69	26824392
276	Glutathionylation	LVDNAYSCDPRIKKF HHHHHHCCCHHHHHH	5.63	24333276
362	Phosphorylation	KLRNSCKSWNHFSDN HHHHHHHHCCCCCCC	37.12	25338131
413	Ubiquitination	VGKAALEKQIEEVNE HHHHHHHHHHHHHHH	58.38	22790023
443	Phosphorylation	ASKNAEKSTGGSGSG HHHHHHHHCCCCCCC	24.64	21149613
444	Phosphorylation	SKNAEKSTGGSGSGS HHHHHHHCCCCCCCC	57.54	21149613
447	Phosphorylation	AEKSTGGSGSGSKNW HHHHCCCCCCCCCCC	31.00	21149613
449	Phosphorylation	KSTGGSGSGSKNWSE HHCCCCCCCCCCCCH	41.41	21149613
451	Phosphorylation	TGGSGSGSKNWSEDD CCCCCCCCCCCCHHH	25.23	21149613
455	Phosphorylation	GSGSKNWSEDDLQLL CCCCCCCCHHHHHHH	40.31	21149613
548	Glutathionylation	SERFEGPCIDSTPWT CHHCCCCCCCCCCCC	8.74	24333276
617	Phosphorylation	QEQVLNASRARK--- HHHHHHHHHHCC---	25.96	28066266

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
47	S	Phosphorylation	Kinase	RPS6KB1	P23443	GPS
47	S	Phosphorylation	Kinase	P70S6K	Q8BSK8	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DNJC2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DNJC2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of DNJC2_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DNJC2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND MASSSPECTROMETRY.	17242355 [Abstract]
"A differential phosphoproteomic analysis of retinoic acid-treated P19cells."; Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.; J. Proteome Res. 6:3174-3186(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; THR-48; SER-49;SER-60 AND SER-63, AND MASS SPECTROMETRY.	17622165 [Abstract]