DGLA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DGLA_HUMAN
• UniProt AC: Q9Y4D2
• Protein Name: Sn1-specific diacylglycerol lipase alpha
• Gene Name: DAGLA
• Organism: Homo sapiens (Human).
• Sequence Length: 1042
• Subcellular Localization: Cell membrane; Multi-pass membrane protein.
• Protein Description: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses..
• Protein Sequence: MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
62	Phosphorylation	LVDHGRGYLGILLSC CCCCCCHHHHHHHHH	10.61	22817900
87	Phosphorylation	SMRGGILYTEPRDSM HHCCCEEEECCCCHH	13.72	30257219
88	Phosphorylation	MRGGILYTEPRDSMQ HCCCEEEECCCCHHH	35.75	30257219
133	N-linked_Glycosylation	CNDLTAKNVTLGMVV CCCCCCCCCCHHHHH	29.55	UniProtKB CARBOHYD
370	Phosphorylation	MTAVDIVYTSCHDAV CCEEEEEEECCCCCC	8.02	22817900
378	Phosphorylation	TSCHDAVYETPFYVA ECCCCCCCCCCEEEE	18.79	22817900
396	Phosphorylation	DKKKVVISIRGTLSP CCCEEEEEEECCCCH	8.47	22964224
400	Phosphorylation	VVISIRGTLSPKDAL EEEEEECCCCHHHHH	17.76	22964224
402	Phosphorylation	ISIRGTLSPKDALTD EEEECCCCHHHHHHH	29.73	24719451
411	Phosphorylation	KDALTDLTGDAERLP HHHHHHCCCCHHHCC	35.51	-
482	Phosphorylation	AGTAAILSFLLRPQY HHHHHHHHHHHCCCC	14.03	24719451
489	Phosphorylation	SFLLRPQYPTLKCFA HHHHCCCCCCCEEEE	10.81	28270605
491	Phosphorylation	LLRPQYPTLKCFAYS HHCCCCCCCEEEEEC	33.64	28270605
497	Phosphorylation	PTLKCFAYSPPGGLL CCCEEEEECCCCCCC	11.06	28270605
498	Phosphorylation	TLKCFAYSPPGGLLS CCEEEEECCCCCCCC	22.61	28270605
505	Phosphorylation	SPPGGLLSEDAMEYS CCCCCCCCHHHHHHC	38.17	28270605
511	Phosphorylation	LSEDAMEYSKEFVTA CCHHHHHHCHHHHHH	16.38	28270605
512	Phosphorylation	SEDAMEYSKEFVTAV CHHHHHHCHHHHHHH	16.65	28270605
550	Ubiquitination	DVLQRSTKPKWRIIV HHHHHCCCCCEEEEE	45.93	30230243
560	Phosphorylation	WRIIVGATKCIPKSE EEEEEECCEECCHHH	22.11	22210691
566	Phosphorylation	ATKCIPKSELPEEVE CCEECCHHHCCCCEE	38.72	-
584	Phosphorylation	LASTRLWTHPSDLTI ECCCCCCCCCCCCEE	28.62	28634298
587	Phosphorylation	TRLWTHPSDLTIALS CCCCCCCCCCEEEEE	37.33	28634298
590	Phosphorylation	WTHPSDLTIALSAST CCCCCCCEEEEECCC	14.81	28634298
594	Phosphorylation	SDLTIALSASTPLYP CCCEEEEECCCCCCC	15.91	28634298
596	Phosphorylation	LTIALSASTPLYPPG CEEEEECCCCCCCCC	27.47	28634298
597	Phosphorylation	TIALSASTPLYPPGR EEEEECCCCCCCCCC	19.61	28634298
600	Phosphorylation	LSASTPLYPPGRIIH EECCCCCCCCCCEEE	14.48	28634298
668	Ubiquitination	KVLENYNKGKTALLS HHHHHCCCHHHHHHH	51.96	29967540
670	Ubiquitination	LENYNKGKTALLSAA HHHCCCHHHHHHHHH	32.03	33845483
675	Phosphorylation	KGKTALLSAAKVMVS CHHHHHHHHHEECCC	27.34	24719451
725	Phosphorylation	HRNSSVRSKSQSEMS CCCHHHCCCCCHHCC	34.21	23312004
727	Phosphorylation	NSSVRSKSQSEMSLE CHHHCCCCCHHCCCC	39.94	22617229
729	Phosphorylation	SVRSKSQSEMSLEGF HHCCCCCHHCCCCCC	42.21	22617229
732	Phosphorylation	SKSQSEMSLEGFSEG CCCCHHCCCCCCCCC	21.15	22617229
743	Phosphorylation	FSEGRLLSPVVAAAA CCCCCCHHHHHHHHH	21.90	29523821
782	Phosphorylation	APLATMESLSDTESL CCCCCCHHHCCCCHH	23.50	23502535
784	Phosphorylation	LATMESLSDTESLYS CCCCHHHCCCCHHHC	52.33	-
797	Phosphorylation	YSFDSRRSSGFRSIR HCCCCCCCCCCCCCC	33.43	22210691
798	Phosphorylation	SFDSRRSSGFRSIRG CCCCCCCCCCCCCCC	39.89	22210691
802	Phosphorylation	RRSSGFRSIRGSPSL CCCCCCCCCCCCCCC	17.86	22210691
806	Phosphorylation	GFRSIRGSPSLHAVL CCCCCCCCCCCEEEE	11.06	28387310
808	Phosphorylation	RSIRGSPSLHAVLER CCCCCCCCCEEEEEC	34.19	30266825
822	Phosphorylation	RDEGHLFYIDPAIPE CCCCEEEEECCCCCC	15.69	27642862
833	Phosphorylation	AIPEENPSLSSRTEL CCCCCCCCCCHHHHH	53.09	30266825
835	Phosphorylation	PEENPSLSSRTELLA CCCCCCCCHHHHHHH	23.31	30266825
836	Phosphorylation	EENPSLSSRTELLAA CCCCCCCHHHHHHHH	48.81	30266825
845	Phosphorylation	TELLAADSLSKHSQD HHHHHHHHHHHCCCC	29.80	-
847	Phosphorylation	LLAADSLSKHSQDTQ HHHHHHHHHCCCCCC	32.07	21815630
850	Phosphorylation	ADSLSKHSQDTQPLE HHHHHHCCCCCCCHH	33.22	26471730
853	Phosphorylation	LSKHSQDTQPLEAAL HHHCCCCCCCHHHHH	25.17	26471730
862	Phosphorylation	PLEAALGSGGVTPER CHHHHHCCCCCCCCC	32.97	26471730
866	Phosphorylation	ALGSGGVTPERPPSA HHCCCCCCCCCCCCC	23.78	26471730
872	Phosphorylation	VTPERPPSAAANDEE CCCCCCCCCCCCCCC	32.97	26471730
905	Phosphorylation	HNGRLGDSPSPQVLE ECCCCCCCCCHHHHH	26.02	27732954
907	Phosphorylation	GRLGDSPSPQVLEFA CCCCCCCCHHHHHHH	31.73	27732954
919	Phosphorylation	EFAEFIDSLFNLDSK HHHHHHHHHCCCCCC	30.00	27732954
925	Phosphorylation	DSLFNLDSKSSSFQD HHHCCCCCCCCCCCC	37.29	27732954
927	Phosphorylation	LFNLDSKSSSFQDLY HCCCCCCCCCCCCEE	34.86	28348404
928	Phosphorylation	FNLDSKSSSFQDLYC CCCCCCCCCCCCEEE	38.62	28348404
929	Phosphorylation	NLDSKSSSFQDLYCM CCCCCCCCCCCEEEE	33.74	28348404
952	Phosphorylation	DYAEGPKSPSQQEIL CCCCCCCCHHHHHHH	32.68	25159151
954	Phosphorylation	AEGPKSPSQQEILLR CCCCCCHHHHHHHHH	52.14	25627689
1021	Phosphorylation	LAADKIRTSTPTGHG HHCCCCCCCCCCCCC	40.19	27794612
1022	Phosphorylation	AADKIRTSTPTGHGA HCCCCCCCCCCCCCC	23.65	27794612
1023	Phosphorylation	ADKIRTSTPTGHGAS CCCCCCCCCCCCCCC	25.02	23312004
1025	Phosphorylation	KIRTSTPTGHGASPA CCCCCCCCCCCCCCC	41.73	28857561
1030	Phosphorylation	TPTGHGASPAKQDEL CCCCCCCCCCCCCCE	29.95	23312004
1040	Phosphorylation	KQDELVISAR----- CCCCEEEECC-----	15.54	23312004

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
782	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
782	S	Phosphorylation	Kinase	CAMK2A	P11798	PSP
808	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
808	S	Phosphorylation	Kinase	CAMK2A	P11798	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
782	S	Diacylglycerol	Phosphorylated at Ser-782 and Ser-808 by CAMK2A; phosphorylation by CAMK2A inhibits diacylglycerol lipase activity.	23502535
782	S	Phosphorylation	Phosphorylated at Ser-782 and Ser-808 by CAMK2A; phosphorylation by CAMK2A inhibits diacylglycerol lipase activity.	23502535
808	S	Diacylglycerol	Phosphorylated at Ser-782 and Ser-808 by CAMK2A; phosphorylation by CAMK2A inhibits diacylglycerol lipase activity.	23502535
808	S	Phosphorylation	Phosphorylated at Ser-782 and Ser-808 by CAMK2A; phosphorylation by CAMK2A inhibits diacylglycerol lipase activity.	23502535

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DGLA_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of DGLA_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 608687: Spinocerebellar ataxia 20 (SCA20)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.