DEND3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DEND3_HUMAN
• UniProt AC: A2RUS2
• Protein Name: DENN domain-containing protein 3
• Gene Name: DENND3
• Organism: Homo sapiens (Human).
• Sequence Length: 1198
• Subcellular Localization: Cytoplasm . Transiently recruited to membranes to activate RAB12.
• Protein Description: Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. [PubMed: 20937701 Regulates autophagy in response to starvation through RAB12 activation. Starvation leads to ULK1/2-dependent phosphorylation of Ser-472 and Ser-490, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards RAB12 (By similarity Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4]
• Protein Sequence: MRSLRKKREKPRPEQWKGLPGPPRAPEPEDVAVPGGVDLLTLPQLCFPGGVCVATEPKEDCVHFLVLTDVCGNRTYGVVAQYYRPLHDEYCFYNGKTHRECPGCFVPFAVCVVSRFPYYNSLKDCLSCLLALLKPCKDFEVDSHIKDFAAKLSLIPSPPPGPLHLVFNMKSLQIVLPARADPESPILDLDLHLPLLCFRPEKVLQILTCILTEQRIVFFSSDWALLTLVTECFMAYLYPLQWQHPFVPILSDQMLDFVMAPTSFLMGCHLDHFEEVSKEADGLVLINIDHGSITYSKSTDDNVDIPDVPLLAAQTFIQRVQSLQLHHELHAAHLLSSTDLKEGRAHRRSWQQKLNCQIQQTTLQLLVSIFRDVKNHLNYEHRVFNSEEFLKTRAPGDHQFYKQVLDTYMFHSFLKARLNRRMDAFAQMDLDTQSEEDRINGMLLSPRRPTVEKRASRKSSHLHVTHRRMVVSMPNLQDIAMPELAPRNSSLRLTDTAGCRGSSAVLNVTPKSPYTFKIPEIHFPLESKCVQAYHAHFVSMLSEAMCFLAPDNSLLLARYLYLRGLVYLMQGQLLNALLDFQNLYKTDIRIFPTDLVKRTVESMSAPEWEGAEQAPELMRLISEILDKPHEASKLDDHVKKFKLPKKHMQLGDFMKRVQESGIVKDASIIHRLFEALTVGQEKQIDPETFKDFYNCWKETEAEAQEVSLPWLVMEHLDKNECVCKLSSSVKTNLGVGKIAMTQKRLFLLTEGRPGYLEISTFRNIEEVRRTTTTFLLRRIPTLKIRVASKKEVFEANLKTECDLWHLMVKEMWAGKKLADDHKDPHYVQQALTNVLLMDAVVGTLQSPGAIYAASKLSYFDKMSNEMPMTLPETTLETLKHKINPSAGEAFPQAVDVLLYTPGHLDPAEKVEDAHPKLWCALSEGKVTVFNASSWTIHQHSFKVGTAKVNCMVMADQNQVWVGSEDSVIYIINVHSMSCNKQLTAHCSSVTDLIVQDGQEAPSNVYSCSMDGMVLVWNVSTLQVTSRFQLPRGGLTSIRLHGGRLWCCTGNSIMVMKMNGSLHQELKIEENFKDTSTSFLAFQLLPEEEQLWAACAGRSEVYIWSLKDLAQPPQRVPLEDCSEINCMIRVKKQVWVGSRGLGQGTPKGKIYVIDAERKTVEKELVAHMDTVRTLCSAEDRYVLSGSGREEGKVAIWKGE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MRSLRKKREK -----CCCCCCCCCC	44.37	26437602
10	Ubiquitination	SLRKKREKPRPEQWK CCCCCCCCCCHHHHC	50.81	-
17	Ubiquitination	KPRPEQWKGLPGPPR CCCHHHHCCCCCCCC	50.71	-
90	Ubiquitination	YRPLHDEYCFYNGKT ECCCCCCEEEECCCC	8.23	21890473
96	Ubiquitination	EYCFYNGKTHRECPG CEEEECCCCCCCCCC	37.20	21890473
103	Ubiquitination	KTHRECPGCFVPFAV CCCCCCCCCCCCEEE	29.20	21890473
109	Ubiquitination	PGCFVPFAVCVVSRF CCCCCCEEEEHHHCC	6.42	21890473
127	Ubiquitination	NSLKDCLSCLLALLK CCHHHHHHHHHHHHH	14.73	23503661
146	Ubiquitination	FEVDSHIKDFAAKLS CCCCHHHHHHHHHHC	41.17	-
176	Ubiquitination	MKSLQIVLPARADPE CCEEEEEEECCCCCC	2.68	22817900
189	Ubiquitination	PESPILDLDLHLPLL CCCCCCCEEECCCEE	7.28	22817900
206	Ubiquitination	RPEKVLQILTCILTE CHHHHHHHHHHHHCC	2.77	23503661
212	Ubiquitination	QILTCILTEQRIVFF HHHHHHHCCCEEEEE	15.34	23503661
218	Ubiquitination	LTEQRIVFFSSDWAL HCCCEEEEECCHHHH	4.59	23503661
219	Ubiquitination	TEQRIVFFSSDWALL CCCEEEEECCHHHHH	4.78	23503661
223	Ubiquitination	IVFFSSDWALLTLVT EEEECCHHHHHHHHH	7.26	23503661
225	Ubiquitination	FFSSDWALLTLVTEC EECCHHHHHHHHHHH	3.06	23503661
231	Ubiquitination	ALLTLVTECFMAYLY HHHHHHHHHHHHHHH	20.48	23503661
247	Ubiquitination	LQWQHPFVPILSDQM HCCCCCCCCCCCHHH	3.20	21890473
253	Ubiquitination	FVPILSDQMLDFVMA CCCCCCHHHHHHHCC	30.84	21890473
309	Ubiquitination	NVDIPDVPLLAAQTF CCCCCCHHHHHHHHH	29.10	21890473
322	Ubiquitination	TFIQRVQSLQLHHEL HHHHHHHHHHHHHHH	18.41	21890473
333	Ubiquitination	HHELHAAHLLSSTDL HHHHHHHHHHCCCCC	28.25	22817900
339 (in isoform 2)	Ubiquitination	-	58.66	21890473
341	Ubiquitination	LLSSTDLKEGRAHRR HHCCCCCCCCHHHHH	62.02	-
343	Ubiquitination	SSTDLKEGRAHRRSW CCCCCCCCHHHHHHH	30.41	21890473
349	Ubiquitination	EGRAHRRSWQQKLNC CCHHHHHHHHHHHCH	29.24	21890473
352	Ubiquitination	AHRRSWQQKLNCQIQ HHHHHHHHHHCHHHH	46.04	23503661
361	Phosphorylation	LNCQIQQTTLQLLVS HCHHHHHHHHHHHHH	17.07	-
363	Ubiquitination	CQIQQTTLQLLVSIF HHHHHHHHHHHHHHH	3.71	23503661
368	Phosphorylation	TTLQLLVSIFRDVKN HHHHHHHHHHHHHHH	18.91	-
369	Ubiquitination	TLQLLVSIFRDVKNH HHHHHHHHHHHHHHH	2.27	23503661
374	Ubiquitination	VSIFRDVKNHLNYEH HHHHHHHHHHCCCCC	42.83	-
375	Ubiquitination	SIFRDVKNHLNYEHR HHHHHHHHHCCCCCC	44.73	23503661
391	Ubiquitination	FNSEEFLKTRAPGDH CCCHHHHHCCCCCCH	41.09	21906983
393	Ubiquitination	SEEFLKTRAPGDHQF CHHHHHCCCCCCHHH	36.88	23503661
401	Phosphorylation	APGDHQFYKQVLDTY CCCCHHHHHHHHHHH	8.24	29083192
404	Ubiquitination	DHQFYKQVLDTYMFH CHHHHHHHHHHHHHH	4.58	23503661
412	Phosphorylation	LDTYMFHSFLKARLN HHHHHHHHHHHHHHH	22.47	24719451
415	Ubiquitination	YMFHSFLKARLNRRM HHHHHHHHHHHHHHH	29.47	-
429	Ubiquitination	MDAFAQMDLDTQSEE HHHHHHCCCCCCCHH	29.34	22817900
445	Phosphorylation	RINGMLLSPRRPTVE HHCCEECCCCCCCHH	16.64	24719451
450	Phosphorylation	LLSPRRPTVEKRASR ECCCCCCCHHHHHCC	39.21	-
456	Phosphorylation	PTVEKRASRKSSHLH CCHHHHHCCCCCCCC	44.25	27273156
459	Phosphorylation	EKRASRKSSHLHVTH HHHHCCCCCCCCEEC	23.14	27273156
459	Ubiquitination	EKRASRKSSHLHVTH HHHHCCCCCCCCEEC	23.14	23503661
459 (in isoform 2)	Ubiquitination	-	23.14	21890473
460	Phosphorylation	KRASRKSSHLHVTHR HHHCCCCCCCCEECC	32.86	27273156
465	Phosphorylation	KSSHLHVTHRRMVVS CCCCCCEECCEEEEE	10.02	28674151
465	Ubiquitination	KSSHLHVTHRRMVVS CCCCCCEECCEEEEE	10.02	23503661
465 (in isoform 2)	Ubiquitination	-	10.02	21890473
466	Ubiquitination	SSHLHVTHRRMVVSM CCCCCEECCEEEEEC	17.79	21890473
471	Ubiquitination	VTHRRMVVSMPNLQD EECCEEEEECCCHHH	2.72	23503661
471 (in isoform 1)	Ubiquitination	-	2.72	21890473
472	Phosphorylation	THRRMVVSMPNLQDI ECCEEEEECCCHHHH	19.67	22115753
472	Ubiquitination	THRRMVVSMPNLQDI ECCEEEEECCCHHHH	19.67	21890473
478	Ubiquitination	VSMPNLQDIAMPELA EECCCHHHHCCCCCC	33.51	21890473
489	Phosphorylation	PELAPRNSSLRLTDT CCCCCCCCCCCEECC	31.84	23403867
490	Phosphorylation	ELAPRNSSLRLTDTA CCCCCCCCCCEECCC	22.51	23401153
494	Phosphorylation	RNSSLRLTDTAGCRG CCCCCCEECCCCCCC	25.52	23403867
496	Phosphorylation	SSLRLTDTAGCRGSS CCCCEECCCCCCCCC	21.51	23403867
502	Phosphorylation	DTAGCRGSSAVLNVT CCCCCCCCCEEEECC	8.86	28450419
503	Phosphorylation	TAGCRGSSAVLNVTP CCCCCCCCEEEECCC	25.81	28450419
509	Phosphorylation	SSAVLNVTPKSPYTF CCEEEECCCCCCCEE	24.75	23401153
511	Ubiquitination	AVLNVTPKSPYTFKI EEEECCCCCCCEEEC	55.48	21890473
512	Phosphorylation	VLNVTPKSPYTFKIP EEECCCCCCCEEECC	25.09	28450419
513	Ubiquitination	LNVTPKSPYTFKIPE EECCCCCCCEEECCC	38.01	21890473
514	Phosphorylation	NVTPKSPYTFKIPEI ECCCCCCCEEECCCE	31.72	28060719
515	Phosphorylation	VTPKSPYTFKIPEIH CCCCCCCEEECCCEE	23.10	28060719
517	Ubiquitination	PKSPYTFKIPEIHFP CCCCCEEECCCEECC	49.94	21890473
519	Ubiquitination	SPYTFKIPEIHFPLE CCCEEECCCEECCCC	34.77	21890473
524	Ubiquitination	KIPEIHFPLESKCVQ ECCCEECCCCCHHHH	22.51	21890473
530	Ubiquitination	FPLESKCVQAYHAHF CCCCCHHHHHHHHHH	4.13	21890473
558	Ubiquitination	DNSLLLARYLYLRGL CCHHHHHHHHHHHHH	23.20	22817900
562	Ubiquitination	LLARYLYLRGLVYLM HHHHHHHHHHHHHHH	3.02	21890473
575 (in isoform 2)	Ubiquitination	-	36.81	-
581 (in isoform 2)	Ubiquitination	-	38.39	-
588	Ubiquitination	QNLYKTDIRIFPTDL HHHHHCCCEEECHHH	4.38	23503661
591 (in isoform 1)	Ubiquitination	-	3.70	21890473
594	Ubiquitination	DIRIFPTDLVKRTVE CCEEECHHHHHHHHH	50.10	23503661
597	Ubiquitination	IFPTDLVKRTVESMS EECHHHHHHHHHHCC	50.04	22817900
597 (in isoform 1)	Ubiquitination	-	50.04	21890473
599	Ubiquitination	PTDLVKRTVESMSAP CHHHHHHHHHHCCCC	23.94	22817900
600	Ubiquitination	TDLVKRTVESMSAPE HHHHHHHHHHCCCCC	6.26	23503661
610	Ubiquitination	MSAPEWEGAEQAPEL CCCCCCCCHHHHHHH	35.17	22817900
622	Phosphorylation	PELMRLISEILDKPH HHHHHHHHHHHCCCC	24.15	24247654
627	Ubiquitination	LISEILDKPHEASKL HHHHHHCCCCHHHCC	44.84	23503661
629	Ubiquitination	SEILDKPHEASKLDD HHHHCCCCHHHCCHH	49.33	23503661
632	Phosphorylation	LDKPHEASKLDDHVK HCCCCHHHCCHHHHH	30.66	23312004
633	Ubiquitination	DKPHEASKLDDHVKK CCCCHHHCCHHHHHH	64.08	23503661
635	Ubiquitination	PHEASKLDDHVKKFK CCHHHCCHHHHHHCC	47.94	23503661
639	Ubiquitination	SKLDDHVKKFKLPKK HCCHHHHHHCCCCHH	49.42	23503661
640	Ubiquitination	KLDDHVKKFKLPKKH CCHHHHHHCCCCHHH	46.79	23503661
641	Ubiquitination	LDDHVKKFKLPKKHM CHHHHHHCCCCHHHC	9.00	23503661
646	Ubiquitination	KKFKLPKKHMQLGDF HHCCCCHHHCCHHHH	42.23	23503661
652	Ubiquitination	KKHMQLGDFMKRVQE HHHCCHHHHHHHHHH	49.40	23503661
655	Ubiquitination	MQLGDFMKRVQESGI CCHHHHHHHHHHCCC	49.78	-
664	Acetylation	VQESGIVKDASIIHR HHHCCCCCCHHHHHH	45.73	20167786
664	Ubiquitination	VQESGIVKDASIIHR HHHCCCCCCHHHHHH	45.73	-
677	Phosphorylation	HRLFEALTVGQEKQI HHHHHHHCCCCCCCC	29.64	24702127
678 (in isoform 2)	Ubiquitination	-	8.51	21890473
682	Ubiquitination	ALTVGQEKQIDPETF HHCCCCCCCCCHHHH	44.67	-
688	Phosphorylation	EKQIDPETFKDFYNC CCCCCHHHHHHHHHH	41.18	-
690	Ubiquitination	QIDPETFKDFYNCWK CCCHHHHHHHHHHHH	55.11	-
691	Ubiquitination	IDPETFKDFYNCWKE CCHHHHHHHHHHHHH	47.25	21890473
718	Ubiquitination	LVMEHLDKNECVCKL HHHHCCCCCCEEEEC	62.09	-
724	Ubiquitination	DKNECVCKLSSSVKT CCCCEEEECCHHHHH	30.89	-
730	Ubiquitination	CKLSSSVKTNLGVGK EECCHHHHHCCCCCC	33.37	21890473
732	Ubiquitination	LSSSVKTNLGVGKIA CCHHHHHCCCCCCEE	29.13	21890473
737	Ubiquitination	KTNLGVGKIAMTQKR HHCCCCCCEEECCCE	25.53	29967540
743	Ubiquitination	GKIAMTQKRLFLLTE CCEEECCCEEEEECC	41.73	21890473
771	Phosphorylation	IEEVRRTTTTFLLRR HHHHHHHHHHHHHHC	23.25	-
772	Phosphorylation	EEVRRTTTTFLLRRI HHHHHHHHHHHHHCC	18.22	-
781	Phosphorylation	FLLRRIPTLKIRVAS HHHHCCCCCEEEECC	38.01	-
790	Ubiquitination	KIRVASKKEVFEANL EEEECCHHHHHHCCC	57.52	-
798	Ubiquitination	EVFEANLKTECDLWH HHHHCCCCCHHHHHH	41.93	-
810 (in isoform 1)	Ubiquitination	-	28.66	21890473
816	Ubiquitination	KEMWAGKKLADDHKD HHHHCCCCCCCCCCC	48.25	-
858	Phosphorylation	YAASKLSYFDKMSNE HHHHHCHHHHHCCCC	26.66	-
861	Ubiquitination	SKLSYFDKMSNEMPM HHCHHHHHCCCCCCC	34.04	-
879	Ubiquitination	ETTLETLKHKINPSA HHHHHHHHHHCCCCH	50.72	-
881	Ubiquitination	TLETLKHKINPSAGE HHHHHHHHCCCCHHH	42.46	-
909	Ubiquitination	GHLDPAEKVEDAHPK CCCCHHHHHCCCCCC	53.70	-
916	Ubiquitination	KVEDAHPKLWCALSE HHCCCCCCCEEEEEC	44.76	-
1131	Ubiquitination	NCMIRVKKQVWVGSR CCEEEEEEEEEECCC	47.42	-
1137	Phosphorylation	KKQVWVGSRGLGQGT EEEEEECCCCCCCCC	17.35	20068231
1144	Phosphorylation	SRGLGQGTPKGKIYV CCCCCCCCCCCEEEE	17.59	20068231
1146	Ubiquitination	GLGQGTPKGKIYVID CCCCCCCCCEEEEEE	73.08	-
1148	Ubiquitination	GQGTPKGKIYVIDAE CCCCCCCEEEEEECC	36.67	-
1161	Ubiquitination	AERKTVEKELVAHMD CCCCCHHHHHHHCHH	52.60	29967540
1172	Phosphorylation	AHMDTVRTLCSAEDR HCHHHHHHHCCHHHC	27.99	24719451
1175	Phosphorylation	DTVRTLCSAEDRYVL HHHHHHCCHHHCEEE	37.49	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
472	S	Phosphorylation	Kinase	ULK1	O75385	Uniprot
472	S	Phosphorylation	Kinase	ULK2	Q8IYT8	PSP
490	S	Phosphorylation	Kinase	ULK1	O75385	Uniprot
490	S	Phosphorylation	Kinase	ULK2	Q8IYT8	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DEND3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DEND3_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of DEND3_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.