dbPTM

DDI2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DDI2_MOUSE
UniProt AC	A2ADY9
Protein Name	Protein DDI1 homolog 2 {ECO:0000305}
Gene Name	Ddi2 {ECO:0000312\|MGI:MGI:1917244}
Organism	Mus musculus (Mouse).
Sequence Length	399
Subcellular Localization	Cytoplasm, cytosol .
Protein Description	Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1..
Protein Sequence	MLLTVYCVRRDLSEVTFSLQVDADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLASYGLKDGDVVILRQKENADPRPAVQFSNLPRIDFSSIAVPGTSNPQQRQLPRTQAQHSSPGEMASSPQGLDNPALLRDMLLANPHELSLLKERNPPLAEALLSGDLEKFSRVLVEQQQDRARREQERIRLFSADPFDLEAQAKIEEDIRQQNIEENMTIAMEEAPESFGQVAMLYINCRVNGHPVKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGIAKGVGTQKIIGRVHLAQVQIEGDFLACSFSILEEQPMDMLLGLDMLKRHQCSIDLKKNVLVIGTTGSQTTFLPEGELPECARLAYGTGREDIRPEEIADQELAEAIQKSAEDAERQKP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
67	Ubiquitination	SLASYGLKDGDVVIL HHHHCCCCCCCEEEE	56.13	22790023
77	Ubiquitination	DVVILRQKENADPRP CEEEEECCCCCCCCC	46.64	22790023
104	Phosphorylation	SSIAVPGTSNPQQRQ CCEECCCCCCHHHCC	21.18	-
115	Phosphorylation	QQRQLPRTQAQHSSP HHCCCCCCCCCCCCC	26.73	24925903
120	Phosphorylation	PRTQAQHSSPGEMAS CCCCCCCCCCCCCCC	26.58	25521595
121	Phosphorylation	RTQAQHSSPGEMASS CCCCCCCCCCCCCCC	34.50	25521595
125	Oxidation	QHSSPGEMASSPQGL CCCCCCCCCCCCCCC	5.63	17242355
127	Phosphorylation	SSPGEMASSPQGLDN CCCCCCCCCCCCCCC	41.04	25521595
128	Phosphorylation	SPGEMASSPQGLDNP CCCCCCCCCCCCCCH	16.23	24925903
150	Phosphorylation	LANPHELSLLKERNP HCCHHHHHHHHHHCC	28.77	23984901
153	Ubiquitination	PHELSLLKERNPPLA HHHHHHHHHHCCHHH	60.98	22790023
170	Ubiquitination	LLSGDLEKFSRVLVE HHHCCHHHHHHHHHH	56.81	22790023
194	Phosphorylation	QERIRLFSADPFDLE HHHHHHEECCCCCHH	36.03	26824392
205	Ubiquitination	FDLEAQAKIEEDIRQ CCHHHHHHHHHHHHH	38.27	22790023
283	Ubiquitination	RRWAGIAKGVGTQKI HHHHCCCCCCCHHHE	52.81	22790023
289	Ubiquitination	AKGVGTQKIIGRVHL CCCCCHHHEECEEEE	35.81	22790023
338	Ubiquitination	QCSIDLKKNVLVIGT CCCCCCCCCEEEEEC	60.63	22790023
389	Ubiquitination	ELAEAIQKSAEDAER HHHHHHHHHHHHHHH	45.58	22790023
390	Phosphorylation	LAEAIQKSAEDAERQ HHHHHHHHHHHHHHH	22.10	30635358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of DDI2_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DDI2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DDI2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of DDI2_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DDI2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120; SER-121 ANDSER-128, AND MASS SPECTROMETRY.	17242355 [Abstract]