dbPTM

DCE2_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DCE2_RAT
UniProt AC	Q05683
Protein Name	Glutamate decarboxylase 2
Gene Name	Gad2
Organism	Rattus norvegicus (Rat).
Sequence Length	585
Subcellular Localization	Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane Lipid-anchor. Golgi apparatus membrane Peripheral membrane protein Cytoplasmic side. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Go
Protein Description	Catalyzes the production of GABA..
Protein Sequence	MASPGSGFWSFGSEDGSGDPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSEKPAESGGSVTSRAATRKVACTCDQKPCSCPKGDVNYALLHATDLLPACEGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLNGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRVLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MASPGSGFWS -----CCCCCCCCCC	28.61	22673903
6	Phosphorylation	--MASPGSGFWSFGS --CCCCCCCCCCCCC	33.91	8999827
10	Phosphorylation	SPGSGFWSFGSEDGS CCCCCCCCCCCCCCC	19.91	22817900
13	Phosphorylation	SGFWSFGSEDGSGDP CCCCCCCCCCCCCCC	30.42	22817900
17	Phosphorylation	SFGSEDGSGDPENPG CCCCCCCCCCCCCCH	52.96	22673903
30	S-palmitoylation	PGTARAWCQVAQKFT CHHHHHHHHHHHHHH	1.97	8132714
45	S-nitrosylation	GGIGNKLCALLYGDS CCHHHHHHHHHHCCC	2.40	22178444
45	S-palmitoylation	GGIGNKLCALLYGDS CCHHHHHHHHHHCCC	2.40	8132714
54	Acetylation	LLYGDSEKPAESGGS HHHCCCCCCCCCCCC	54.18	22902405
78	Ubiquitination	VACTCDQKPCSCPKG EEEECCCCCCCCCCC	34.44	-
95	Phosphorylation	NYALLHATDLLPACE CHHEEEHHHCHHHCC	19.21	-
286	Acetylation	EHSHFSLKKGAAALG CCCCCCCCCCHHHCC	48.22	22902405
396	N6-(pyridoxal phosphate)lysine	SVTWNPHKMMGVPLQ CCCCCHHHCCCCCCC	31.66	-
396	Other	SVTWNPHKMMGVPLQ CCCCCHHHCCCCCCC	31.66	-
417	Phosphorylation	REEGLMQSCNQMHAS CHHHHHHHHHHHHHH	10.89	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of DCE2_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DCE2_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DCE2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of DCE2_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DCE2_RAT

Related Literatures of Post-Translational Modification

Palmitoylation
Reference	PubMed
"Amino acid residues 24-31 but not palmitoylation of cysteines 30 and45 are required for membrane anchoring of glutamic acid decarboxylase,GAD65."; Shi Y., Veit B., Baekkeskov S.; J. Cell Biol. 124:927-934(1994). Cited for: PALMITOYLATION AT CYS-30 AND CYS-45, AND MUTAGENESIS OF CYS-30;CYS-45; CYS-73; CYS-75; CYS-80; CYS-82 AND CYS-101.	8132714 [Abstract]
"Membrane anchoring of the autoantigen GAD65 to microvesicles inpancreatic beta-cells by palmitoylation in the NH2-terminal domain."; Christgau S., Aanstoot H.-J., Schierbeck H., Begley K., Tullin S.,Hejnaes K., Baekkeskov S.; J. Cell Biol. 118:309-320(1992). Cited for: SUBCELLULAR LOCATION, AND PALMITOYLATION.	1321158 [Abstract]
Phosphorylation
Reference	PubMed
"Phosphorylation of serine residues 3, 6, 10, and 13 distinguishesmembrane anchored from soluble glutamic acid decarboxylase 65 and isrestricted to glutamic acid decarboxylase 65alpha."; Namchuk M., Lindsay L., Turck C.W., Kanaani J., Baekkeskov S.; J. Biol. Chem. 272:1548-1557(1997). Cited for: PHOSPHORYLATION AT SER-3; SER-6; SER-10 AND SER-13, ANDBIOPHYSICOCHEMICAL PROPERTIES.	8999827 [Abstract]