dbPTM

CYTSA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CYTSA_HUMAN
UniProt AC	Q69YQ0
Protein Name	Cytospin-A
Gene Name	SPECC1L
Organism	Homo sapiens (Human).
Sequence Length	1117
Subcellular Localization	Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Cell junction, gap junction . Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microt
Protein Description	Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration..
Protein Sequence	MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPDNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEVKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MKKASRSVGSVP ---CCCCCCCCCCCC	33.69	22210691
7	Phosphorylation	-MKKASRSVGSVPKV -CCCCCCCCCCCCCC	28.47	22210691
10	Phosphorylation	KASRSVGSVPKVSAI CCCCCCCCCCCCCEE	32.84	22210691
20	Phosphorylation	KVSAISKTQTAEKIK CCCEECCCCCCHHCC	25.10	-
31	Phosphorylation	EKIKPENSSSASTGG HHCCCCCCCCCCCCC	24.81	28387310
33	Phosphorylation	IKPENSSSASTGGKL CCCCCCCCCCCCCCC	26.51	27251275
35	Phosphorylation	PENSSSASTGGKLVK CCCCCCCCCCCCCCC	29.54	28348404
36	Phosphorylation	ENSSSASTGGKLVKP CCCCCCCCCCCCCCC	50.06	28387310
48	Phosphorylation	VKPGTAASLSKTKSS CCCCCHHHHCCCCCC	30.61	27251275
52	Phosphorylation	TAASLSKTKSSDDLL CHHHHCCCCCCCCCH	32.55	30266825
54	Phosphorylation	ASLSKTKSSDDLLAG HHHCCCCCCCCCHHH	44.20	30266825
55	Phosphorylation	SLSKTKSSDDLLAGM HHCCCCCCCCCHHHH	36.38	30266825
67	Phosphorylation	AGMAGGVTVTNGVKG HHHCCCEEEECCCCC	25.53	23927012
69	Phosphorylation	MAGGVTVTNGVKGKK HCCCEEEECCCCCCC	19.52	23927012
78	Phosphorylation	GVKGKKSTCPSAAPS CCCCCCCCCCCCCCC	37.00	28555341
93	Phosphorylation	ASAPAMTTVENKSKI CCCCCEEEECCCCCC	16.85	-
102	Phosphorylation	ENKSKISTGTASSTK CCCCCCCCCCCCCCC	41.86	23403867
104	Phosphorylation	KSKISTGTASSTKRS CCCCCCCCCCCCCCC	24.37	23403867
106	Phosphorylation	KISTGTASSTKRSTS CCCCCCCCCCCCCCC	38.08	23403867
107	Phosphorylation	ISTGTASSTKRSTST CCCCCCCCCCCCCCC	35.17	23403867
108	Phosphorylation	STGTASSTKRSTSTG CCCCCCCCCCCCCCC	28.16	23403867
109	Acetylation	TGTASSTKRSTSTGN CCCCCCCCCCCCCCC	46.42	30592605
113	Phosphorylation	SSTKRSTSTGNKESS CCCCCCCCCCCCCCH	35.25	-
114	Phosphorylation	STKRSTSTGNKESSS CCCCCCCCCCCCCHH	44.88	-
120	Phosphorylation	STGNKESSSTRERLR CCCCCCCHHHHHHHH	36.50	-
122	Phosphorylation	GNKESSSTRERLRER CCCCCHHHHHHHHHH	38.20	-
140	Phosphorylation	NQSKKLPSAGQGAND HHCCCCCCCCCCHHH	56.31	28348404
156	Phosphorylation	ALAKRSRSRTATECD HHHHHHHCCCCCHHH	34.92	27251275
158	Phosphorylation	AKRSRSRTATECDVR HHHHHCCCCCHHHHH	38.90	27251275
160	Phosphorylation	RSRSRTATECDVRMS HHHCCCCCHHHHHCC	36.21	-
169	Phosphorylation	CDVRMSKSKSDNQIS HHHHCCCCCCCCCCC	30.04	21406692
171	Phosphorylation	VRMSKSKSDNQISDR HHCCCCCCCCCCCHH	49.21	26657352
176	Phosphorylation	SKSDNQISDRAALEA CCCCCCCCHHHHHHH	15.98	28102081
195	Acetylation	LLTLAKTKDVEILHL HHHHHHCCCCCHHHH	60.10	26051181
220	Phosphorylation	LGINEDHSEGDEKSE HCCCCCCCCCCHHHH	56.44	29255136
226	Phosphorylation	HSEGDEKSEKETIMA CCCCCHHHHHHHHHH	52.43	29255136
277	O-linked_Glycosylation	RLNALGFSLEQRLDN HHHHHCCCHHHHCCC	29.24	30379171
277	Phosphorylation	RLNALGFSLEQRLDN HHHHHCCCHHHHCCC	29.24	28857561
295	Phosphorylation	LFGYQSLSPEITPDN HCCEECCCCCCCCCC	26.24	24275569
357	Phosphorylation	SEVYQPLTSSDDALD HHHHCCCCCCCCCCC	32.63	24275569
367	Phosphorylation	DDALDAPSSSESEGI CCCCCCCCCCCCCCC	48.49	24275569
371	Phosphorylation	DAPSSSESEGIPSIE CCCCCCCCCCCCCCC	42.97	24275569
380	Phosphorylation	GIPSIERSRKGSSGN CCCCCCCCCCCCCCC	26.08	23403867
382	Ubiquitination	PSIERSRKGSSGNAS CCCCCCCCCCCCCHH	65.47	-
384	Phosphorylation	IERSRKGSSGNASEV CCCCCCCCCCCHHHH	37.80	29255136
385	Phosphorylation	ERSRKGSSGNASEVS CCCCCCCCCCHHHHH	44.74	23927012
389	Phosphorylation	KGSSGNASEVSVACL CCCCCCHHHHHHHHH	42.40	23927012
392	Phosphorylation	SGNASEVSVACLTER CCCHHHHHHHHHHHH	10.43	29209046
397	Phosphorylation	EVSVACLTERIHQME HHHHHHHHHHHHHHH	24.09	23927012
448	Phosphorylation	EKVILMESLCQQSDK HHHHHHHHHHHCHHH	22.08	27251275
576	Sulfoxidation	VASDQIEMNRLKAQL HHHHHHHHHHHHHHH	3.65	21406390
594	Phosphorylation	KQKVAELYSIHNSGD HHHHHHHHHHHCCCC	9.33	25159151
599	Phosphorylation	ELYSIHNSGDKSDIQ HHHHHHCCCCHHHHH	33.92	25159151
603	Phosphorylation	IHNSGDKSDIQDLLE HHCCCCHHHHHHHHH	44.69	26307563
618	2-Hydroxyisobutyrylation	SVRLDKEKAETLASS HHCCCHHHHHHHHHH	58.21	-
618	Ubiquitination	SVRLDKEKAETLASS HHCCCHHHHHHHHHH	58.21	-
668	Phosphorylation	QIEDLNMTLEKLRSD HHHHHHHHHHHHHHC	30.60	21815630
687	Ubiquitination	ETERSDMKETIFELE HHHHHHHHHHHHHHH	57.61	-
712	Phosphorylation	LHDNLIISDLENTVK CCCCEEHHHHHHHHH	29.20	-
743	Phosphorylation	HRRLREESAEWRQFQ HHHHHHHHHHHHHHH	27.08	27251275
775	Ubiquitination	QEEIGDLKRRLHEAQ HHHHHHHHHHHHHHH	39.97	-
808	Phosphorylation	EEERGRVYNYMNAVE HHHHHHHHHHHHHHH	10.14	22817900
810	Phosphorylation	ERGRVYNYMNAVERD HHHHHHHHHHHHHHH	3.66	22817900
828	Phosphorylation	LRQGMGLSRRSSTSS HHHCCCCCCCCCCCC	21.66	24719451
831	Phosphorylation	GMGLSRRSSTSSEPT CCCCCCCCCCCCCCC	36.54	29255136
832	Phosphorylation	MGLSRRSSTSSEPTP CCCCCCCCCCCCCCC	30.42	29255136
833	Phosphorylation	GLSRRSSTSSEPTPT CCCCCCCCCCCCCCH	37.20	27273156
834	Phosphorylation	LSRRSSTSSEPTPTV CCCCCCCCCCCCCHH	34.35	29255136
835	Phosphorylation	SRRSSTSSEPTPTVK CCCCCCCCCCCCHHH	47.92	23927012
838	Phosphorylation	SSTSSEPTPTVKTLI CCCCCCCCCHHHHHH	27.78	23927012
840	Phosphorylation	TSSEPTPTVKTLIKS CCCCCCCHHHHHHHH	36.79	23927012
842	Ubiquitination	SEPTPTVKTLIKSFD CCCCCHHHHHHHHCC	39.84	21890473
842	Ubiquitination	SEPTPTVKTLIKSFD CCCCCHHHHHHHHCC	39.84	21890473
843	Phosphorylation	EPTPTVKTLIKSFDS CCCCHHHHHHHHCCC	29.40	29083192
846	Ubiquitination	PTVKTLIKSFDSASQ CHHHHHHHHCCCHHC	48.46	21890473
846	Ubiquitination	PTVKTLIKSFDSASQ CHHHHHHHHCCCHHC	48.46	21890473
847	Phosphorylation	TVKTLIKSFDSASQV HHHHHHHHCCCHHCC	27.56	29507054
850	Phosphorylation	TLIKSFDSASQVPNP HHHHHCCCHHCCCCC	28.01	22199227
852	Phosphorylation	IKSFDSASQVPNPAA HHHCCCHHCCCCCHH	35.35	27251275
865	Phosphorylation	AAAAIPRTPLSPSPM HHHCCCCCCCCCCCC	24.19	30266825
868	Phosphorylation	AIPRTPLSPSPMKTP CCCCCCCCCCCCCCC	25.24	30266825
870	Phosphorylation	PRTPLSPSPMKTPPA CCCCCCCCCCCCCCC	33.96	30266825
874	Phosphorylation	LSPSPMKTPPAAAVS CCCCCCCCCCCCCCC	28.40	23927012
881	Phosphorylation	TPPAAAVSPMQRHSI CCCCCCCCCCCCCCC	15.21	30266825
887	Phosphorylation	VSPMQRHSISGPIST CCCCCCCCCCCCCCC	22.19	23401153
889	Phosphorylation	PMQRHSISGPISTSK CCCCCCCCCCCCCCC	41.34	29255136
893	Phosphorylation	HSISGPISTSKPLTA CCCCCCCCCCCCCHH	29.75	25159151
894	Phosphorylation	SISGPISTSKPLTAL CCCCCCCCCCCCHHH	41.30	20068231
895	Phosphorylation	ISGPISTSKPLTALS CCCCCCCCCCCHHHC	26.03	20068231
896	Ubiquitination	SGPISTSKPLTALSD CCCCCCCCCCHHHCC	44.10	21890473
896	Malonylation	SGPISTSKPLTALSD CCCCCCCCCCHHHCC	44.10	26320211
896	Ubiquitination	SGPISTSKPLTALSD CCCCCCCCCCHHHCC	44.10	21890473
902	Phosphorylation	SKPLTALSDKRPNYG CCCCHHHCCCCCCCC	38.33	22199227
904	Ubiquitination	PLTALSDKRPNYGEI CCHHHCCCCCCCCCC	67.39	21890473
904	Ubiquitination	PLTALSDKRPNYGEI CCHHHCCCCCCCCCC	67.39	21890473
908	Phosphorylation	LSDKRPNYGEIPVQE HCCCCCCCCCCCHHH	20.86	29978859
920	Phosphorylation	VQEHLLRTSSASRPA HHHHHHHCCCCCCCC	27.31	28555341
921	Phosphorylation	QEHLLRTSSASRPAS HHHHHHCCCCCCCCC	19.92	21712546
922	Phosphorylation	EHLLRTSSASRPASL HHHHHCCCCCCCCCC	29.65	25159151
924	Phosphorylation	LLRTSSASRPASLPR HHHCCCCCCCCCCCC	40.52	23312004
928	Phosphorylation	SSASRPASLPRVPAM CCCCCCCCCCCCCHH	41.40	25159151
937	Phosphorylation	PRVPAMESAKTLSVS CCCCHHHCCCCCCCC	23.21	-
939	Ubiquitination	VPAMESAKTLSVSRR CCHHHCCCCCCCCCC	60.31	21890473
939	Ubiquitination	VPAMESAKTLSVSRR CCHHHCCCCCCCCCC	60.31	21890473
942	Phosphorylation	MESAKTLSVSRRSSE HHCCCCCCCCCCCHH	24.23	28060719
944	Phosphorylation	SAKTLSVSRRSSEEV CCCCCCCCCCCHHHH	20.71	24719451
948	Phosphorylation	LSVSRRSSEEVKRDI CCCCCCCHHHHHHHH	35.64	-
956	Phosphorylation	EEVKRDISAQEGASP HHHHHHHHHHCCCCH	28.34	27251275
962	Phosphorylation	ISAQEGASPASLMAM HHHHCCCCHHHHHHC	31.68	23090842
965	Phosphorylation	QEGASPASLMAMGTT HCCCCHHHHHHCCCC	23.28	23090842
971	Phosphorylation	ASLMAMGTTSPQLSL HHHHHCCCCCCCCCC	15.83	30278072
972	Phosphorylation	SLMAMGTTSPQLSLS HHHHCCCCCCCCCCC	32.45	30278072
973	Phosphorylation	LMAMGTTSPQLSLSS HHHCCCCCCCCCCCC	15.53	20068231
977	Phosphorylation	GTTSPQLSLSSSPTA CCCCCCCCCCCCCCC	22.43	27050516
979	Phosphorylation	TSPQLSLSSSPTASV CCCCCCCCCCCCCCC	25.95	20068231
980	Phosphorylation	SPQLSLSSSPTASVT CCCCCCCCCCCCCCC	45.70	20068231
981	Phosphorylation	PQLSLSSSPTASVTP CCCCCCCCCCCCCCC	23.93	30576142
983	Phosphorylation	LSLSSSPTASVTPTT CCCCCCCCCCCCCCC	33.25	30278072
985	Phosphorylation	LSSSPTASVTPTTRS CCCCCCCCCCCCCHH	29.06	30278072
987	Phosphorylation	SSPTASVTPTTRSRI CCCCCCCCCCCHHHH	16.66	20068231
989	Phosphorylation	PTASVTPTTRSRIRE CCCCCCCCCHHHHHH	26.02	20068231
990	Phosphorylation	TASVTPTTRSRIREE CCCCCCCCHHHHHHH	28.05	20068231
992	Phosphorylation	SVTPTTRSRIREERK CCCCCCHHHHHHHHC	29.93	22210691
999	Ubiquitination	SRIREERKDPLSALA HHHHHHHCCHHHHHH	67.96	-
1003	Phosphorylation	EERKDPLSALAREYG HHHCCHHHHHHHHHC	26.99	21815630
1013	Acetylation	AREYGGSKRNALLKW HHHHCCHHHHHHHHH	53.60	-
1019	Acetylation	SKRNALLKWCQKKTE HHHHHHHHHHHHHCC	46.93	-
1081	Phosphorylation	LAFQAAESVGIKSTL HHHHHHHHCCCCCCC	22.40	-
1085	Acetylation	AAESVGIKSTLDINE HHHHCCCCCCCCHHH	31.31	19823681

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of CYTSA_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CYTSA_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CYTSA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of CYTSA_HUMAN !!

Drug and Disease Associations

Kegg Disease
OMIM Disease
600251	Facial clefting, oblique, 1 (OBLFC1)
145410	Opitz GBBB syndrome 2 (GBBB2)
Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CYTSA_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-868, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-881, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-832; SER-868; THR-874AND SER-881, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-881, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, AND MASSSPECTROMETRY.	17081983 [Abstract]