CNR1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CNR1_HUMAN
• UniProt AC: P21554
• Protein Name: Cannabinoid receptor 1
• Gene Name: CNR1
• Organism: Homo sapiens (Human).
• Sequence Length: 472
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Membrane raft . Mitochondrion outer membrane . Cell projection, axon . Unexpectedly, in the mitochondria, the C-terminus is located in the mitochondrial intermembrane space, a compartment topologically co
• Protein Description: G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). [PubMed: 15620723]
• Protein Sequence: MKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNPQLVPADQVNITEFYNKSLSSFKENEENIQCGENFMDIECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSRSLRCRPSYHFIGSLAVADLLGSVIFVYSFIDFHVFHRKDSRNVFLFKLGGVTASFTASVGSLFLTAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIFPHIDETYLMFWIGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MKSILDGLAD -----CCHHHHHHCC	27.38	24719451
15	Phosphorylation	LADTTFRTITTDLLY HCCCCCCCCCCCEEE	20.63	-
51	Phosphorylation	FPQKFPLTSFRGSPF CCCCCCCCCCCCCCH	26.51	22210691
52	Phosphorylation	PQKFPLTSFRGSPFQ CCCCCCCCCCCCCHH	22.19	22210691
77	N-linked_Glycosylation	LVPADQVNITEFYNK CCCHHHCCHHHHHHH	29.81	UniProtKB CARBOHYD
83	N-linked_Glycosylation	VNITEFYNKSLSSFK CCHHHHHHHHHHHHH	32.53	UniProtKB CARBOHYD
112	N-linked_Glycosylation	IECFMVLNPSQQLAI EEEEEEECHHHHHHH	23.83	UniProtKB CARBOHYD
224	Phosphorylation	SIHRPLAYKRIVTRP HCCCCCCHHHCCCCH	14.42	-
316	Phosphorylation	IQRGTQKSIIIHTSE HHCCCCCEEEEEECC	14.81	30108239
322	Phosphorylation	KSIIIHTSEDGKVQV CEEEEEECCCCCEEE	21.37	25307156
344	Phosphorylation	MDIRLAKTLVLILVV HHHHHHHHHHHHHHH	19.25	-
402	Acetylation	IIYALRSKDLRHAFR HHHHHHCCHHHHHHH	55.11	10079092
415	S-palmitoylation	FRSMFPSCEGTAQPL HHHHCCCCCCCCCCC	5.95	21895628
425	Phosphorylation	TAQPLDNSMGDSDCL CCCCCCCCCCCHHHH	24.65	22817900
429	Phosphorylation	LDNSMGDSDCLHKHA CCCCCCCHHHHHHHH	25.21	22817900
460	Phosphorylation	TVKIAKVTMSVSTDT HHEEEEEEEEEECCC	11.74	-
464	Phosphorylation	AKVTMSVSTDTSAEA EEEEEEEECCCCHHC	17.17	24260401
465	Phosphorylation	KVTMSVSTDTSAEAL EEEEEEECCCCHHCC	40.93	-
467	Phosphorylation	TMSVSTDTSAEAL-- EEEEECCCCHHCC--	29.89	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CNR1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
415	C	Palmitoylation	Palmitoylation at Cys-415 is important for recruitment at plasma membrane and lipid rafts and association with G protein alpha subunits.	21895628

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CNR1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GNAI1_HUMAN	GNAI1	physical	11168387
GNAI2_HUMAN	GNAI2	physical	11168387
GNAI3_HUMAN	GNAI3	physical	11168387

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• D00306: Dronabinol (USP/INN); Tetrahydrocannabinol; Marinol (TN)
• D04717: Levonantradol hydrochloride (USAN)
• D05099: Nabilone (USAN/INN); Cesamet (TN)
• D05731: Rimonabant (JAN/USAN/INN); Acomplia (TN)
• D09009: Taranabant (USAN/INN)
• D09349: Ibipinabant (USAN/INN)
• D09362: Otenabant (USAN/INN)
• D09363: Otenabant hydrochloride (USAN)
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Palmitoylation

"Effects of palmitoylation of Cys(415) in helix 8 of the CB(1)cannabinoid receptor on membrane localization and signalling.";
Oddi S., Dainese E., Sandiford S., Fezza F., Lanuti M., Chiurchiu V.,Totaro A., Catanzaro G., Barcaroli D., De Laurenzi V., Centonze D.,Mukhopadhyay S., Selent J., Howlett A.C., Maccarrone M.;
Br. J. Pharmacol. 165:2635-2651(2011).
Cited for: PALMITOYLATION AT CYS-415.
PubMed: 21895628