dbPTM

CKP2L_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CKP2L_HUMAN
UniProt AC	Q8IYA6
Protein Name	Cytoskeleton-associated protein 2-like
Gene Name	CKAP2L
Organism	Homo sapiens (Human).
Sequence Length	745
Subcellular Localization	Cytoplasm, cytoskeleton, spindle pole . Uniformly distributed along each microtubule bundle of spindles in addition to centrioles during mitosis, expression promptly diminishes at interphase.
Protein Description	Microtubule-associated protein required for mitotic spindle formation and cell-cycle progression in neural progenitor cells..
Protein Sequence	MVGPGPTAAAAVEERQRKLQEYLAAKGKLKSQNTKPYLKSKNNCQNQPPSKSTIRPKNDVTNHVVLPVKPKRSISIKLQPRPPNTAGSQKPKLEPPKLLGKRLTSECVSSNPYSKPSSKSFQQCEAGSSTTGELSRKPVGSLNIEQLKTTKQQLTDQGNGKCIDFMNNIHVENESLDNFLKETNKENLLDILTEPERKPDPKLYTRSKPKTDSYNQTKNSLVPKQALGKSSVNSAVLKDRVNKQFVGETQSRTFPVKSQQLSRGADLARPGVKPSRTVPSHFIRTLSKVQSSKKPVVKNIKDIKVNRSQYERPNETKIRSYPVTEQRVKHTKPRTYPSLLQGEYNNRHPNIKQDQKSSQVCIPQTSCVLQKSKAISQRPNLTVGRFNSAIPSTPSIRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEEEEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLASKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGFQTPES

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Ubiquitination	AVEERQRKLQEYLAA HHHHHHHHHHHHHHH	46.30	29967540
20	Ubiquitination	EERQRKLQEYLAAKG HHHHHHHHHHHHHHC	40.08	29967540
22	Phosphorylation	RQRKLQEYLAAKGKL HHHHHHHHHHHHCCC	6.67	27642862
26	Acetylation	LQEYLAAKGKLKSQN HHHHHHHHCCCCCCC	51.47	25953088
26	Ubiquitination	LQEYLAAKGKLKSQN HHHHHHHHCCCCCCC	51.47	29967540
30	Methylation	LAAKGKLKSQNTKPY HHHHCCCCCCCCCHH	54.18	-
33	Ubiquitination	KGKLKSQNTKPYLKS HCCCCCCCCCHHCCC	58.10	29967540
35	Acetylation	KLKSQNTKPYLKSKN CCCCCCCCHHCCCCC	38.93	26051181
37	Phosphorylation	KSQNTKPYLKSKNNC CCCCCCHHCCCCCCC	28.46	29496907
37	Ubiquitination	KSQNTKPYLKSKNNC CCCCCCHHCCCCCCC	28.46	29967540
39	Methylation	QNTKPYLKSKNNCQN CCCCHHCCCCCCCCC	54.24	-
53	Phosphorylation	NQPPSKSTIRPKNDV CCCCCCCCCCCCCCC	25.41	-
53	Ubiquitination	NQPPSKSTIRPKNDV CCCCCCCCCCCCCCC	25.41	29967540
59	Ubiquitination	STIRPKNDVTNHVVL CCCCCCCCCCCCEEE	55.53	33845483
64	Ubiquitination	KNDVTNHVVLPVKPK CCCCCCCEEEECCCC	5.26	29967540
69	Acetylation	NHVVLPVKPKRSISI CCEEEECCCCCEEEE	42.77	25953088
69	Ubiquitination	NHVVLPVKPKRSISI CCEEEECCCCCEEEE	42.77	33845483
73	Phosphorylation	LPVKPKRSISIKLQP EECCCCCEEEEECCC	27.15	24719451
73	Ubiquitination	LPVKPKRSISIKLQP EECCCCCEEEEECCC	27.15	29967540
75	Phosphorylation	VKPKRSISIKLQPRP CCCCCEEEEECCCCC	18.21	24719451
78	Ubiquitination	KRSISIKLQPRPPNT CCEEEEECCCCCCCC	8.92	29967540
92	Ubiquitination	TAGSQKPKLEPPKLL CCCCCCCCCCCCCCC	72.94	-
92	Ubiquitination	TAGSQKPKLEPPKLL CCCCCCCCCCCCCCC	72.94	23000965
104	Phosphorylation	KLLGKRLTSECVSSN CCCCCHHHHHHHHCC	26.89	28192239
105	Phosphorylation	LLGKRLTSECVSSNP CCCCHHHHHHHHCCC	33.32	28192239
108	Ubiquitination	KRLTSECVSSNPYSK CHHHHHHHHCCCCCC	6.54	29967540
109	Phosphorylation	RLTSECVSSNPYSKP HHHHHHHHCCCCCCC	35.74	20068231
110	Phosphorylation	LTSECVSSNPYSKPS HHHHHHHCCCCCCCC	22.79	20068231
113	Phosphorylation	ECVSSNPYSKPSSKS HHHHCCCCCCCCCCC	33.31	20068231
114	Phosphorylation	CVSSNPYSKPSSKSF HHHCCCCCCCCCCCC	39.07	20068231
115	Ubiquitination	VSSNPYSKPSSKSFQ HHCCCCCCCCCCCCC	42.97	29967540
117	Phosphorylation	SNPYSKPSSKSFQQC CCCCCCCCCCCCCCC	54.88	-
118	Phosphorylation	NPYSKPSSKSFQQCE CCCCCCCCCCCCCCC	40.63	-
119	Ubiquitination	PYSKPSSKSFQQCEA CCCCCCCCCCCCCCC	60.68	29967540
120	Phosphorylation	YSKPSSKSFQQCEAG CCCCCCCCCCCCCCC	30.18	29978859
129	Phosphorylation	QQCEAGSSTTGELSR CCCCCCCCCCCCCCC	29.13	20860994
137	Ubiquitination	TTGELSRKPVGSLNI CCCCCCCCCCCCCCH	40.45	29967540
141	Phosphorylation	LSRKPVGSLNIEQLK CCCCCCCCCCHHHHH	20.62	20860994
148	Ubiquitination	SLNIEQLKTTKQQLT CCCHHHHHHHHHHHH	54.57	29967540
149	Phosphorylation	LNIEQLKTTKQQLTD CCHHHHHHHHHHHHH	48.06	25278378
150	Phosphorylation	NIEQLKTTKQQLTDQ CHHHHHHHHHHHHHC	25.85	25278378
151	Ubiquitination	IEQLKTTKQQLTDQG HHHHHHHHHHHHHCC	41.08	29967540
152	Ubiquitination	EQLKTTKQQLTDQGN HHHHHHHHHHHHCCC	41.23	29967540
155	Phosphorylation	KTTKQQLTDQGNGKC HHHHHHHHHCCCCEE	23.18	25278378
161	Ubiquitination	LTDQGNGKCIDFMNN HHHCCCCEEEECCCC	31.83	29967540
175	Phosphorylation	NIHVENESLDNFLKE CEEECCHHHHHHHHH	53.24	26074081
185	Ubiquitination	NFLKETNKENLLDIL HHHHHHCHHCHHHHH	56.23	29967540
187	Ubiquitination	LKETNKENLLDILTE HHHHCHHCHHHHHHC	47.73	29967540
193	Phosphorylation	ENLLDILTEPERKPD HCHHHHHHCCCCCCC	50.24	20068231
198	Sumoylation	ILTEPERKPDPKLYT HHHCCCCCCCCCCCC	52.70	-
198	Sumoylation	ILTEPERKPDPKLYT HHHCCCCCCCCCCCC	52.70	28112733
198	Ubiquitination	ILTEPERKPDPKLYT HHHCCCCCCCCCCCC	52.70	29967540
202	Ubiquitination	PERKPDPKLYTRSKP CCCCCCCCCCCCCCC	62.60	29967540
204	Phosphorylation	RKPDPKLYTRSKPKT CCCCCCCCCCCCCCC	13.26	29083192
205	Phosphorylation	KPDPKLYTRSKPKTD CCCCCCCCCCCCCCC	38.61	29083192
207	O-linked_Glycosylation	DPKLYTRSKPKTDSY CCCCCCCCCCCCCCC	45.57	30379171
207	Phosphorylation	DPKLYTRSKPKTDSY CCCCCCCCCCCCCCC	45.57	29083192
213	O-linked_Glycosylation	RSKPKTDSYNQTKNS CCCCCCCCCCCCCCC	31.08	30379171
217	O-linked_Glycosylation	KTDSYNQTKNSLVPK CCCCCCCCCCCCCCH	28.67	30379171
218	Ubiquitination	TDSYNQTKNSLVPKQ CCCCCCCCCCCCCHH	34.47	29967540
224	Ubiquitination	TKNSLVPKQALGKSS CCCCCCCHHHCCCCC	40.50	33845483
229	Ubiquitination	VPKQALGKSSVNSAV CCHHHCCCCCCCHHH	40.17	29967540
234	O-linked_Glycosylation	LGKSSVNSAVLKDRV CCCCCCCHHHHHHHH	20.00	30379171
238	Ubiquitination	SVNSAVLKDRVNKQF CCCHHHHHHHHHHHH	36.57	29967540
243	Ubiquitination	VLKDRVNKQFVGETQ HHHHHHHHHHCCCCC	42.11	29967540
249	Phosphorylation	NKQFVGETQSRTFPV HHHHCCCCCCCEECC	26.39	30242111
251	Phosphorylation	QFVGETQSRTFPVKS HHCCCCCCCEECCCH	40.48	30242111
253	Phosphorylation	VGETQSRTFPVKSQQ CCCCCCCEECCCHHH	37.22	29083192
257	Ubiquitination	QSRTFPVKSQQLSRG CCCEECCCHHHHCCC	42.67	23000965
258	Phosphorylation	SRTFPVKSQQLSRGA CCEECCCHHHHCCCC	24.32	29083192
259	Ubiquitination	RTFPVKSQQLSRGAD CEECCCHHHHCCCCC	42.38	29967540
262	Phosphorylation	PVKSQQLSRGADLAR CCCHHHHCCCCCCCC	25.08	29083192
269	Ubiquitination	SRGADLARPGVKPSR CCCCCCCCCCCCCCC	35.48	29967540
273	Ubiquitination	DLARPGVKPSRTVPS CCCCCCCCCCCCCCH	43.10	-
273	Ubiquitination	DLARPGVKPSRTVPS CCCCCCCCCCCCCCH	43.10	29967540
287	Phosphorylation	SHFIRTLSKVQSSKK HHHHHHHHHHHCCCC	30.12	-
288	Acetylation	HFIRTLSKVQSSKKP HHHHHHHHHHCCCCC	47.62	25953088
292	Ubiquitination	TLSKVQSSKKPVVKN HHHHHHCCCCCCCCC	27.84	-
301	Ubiquitination	KPVVKNIKDIKVNRS CCCCCCHHHCCCCHH	63.90	29967540
308	Ubiquitination	KDIKVNRSQYERPNE HHCCCCHHHCCCCCC	31.73	29967540
310	Phosphorylation	IKVNRSQYERPNETK CCCCHHHCCCCCCCC	18.36	28796482
315	Ubiquitination	SQYERPNETKIRSYP HHCCCCCCCCCCCCC	54.91	29967540
317	Ubiquitination	YERPNETKIRSYPVT CCCCCCCCCCCCCCC	29.91	29967540
320	Phosphorylation	PNETKIRSYPVTEQR CCCCCCCCCCCCHHH	36.94	26462736
321	Phosphorylation	NETKIRSYPVTEQRV CCCCCCCCCCCHHHC	7.59	-
324	Phosphorylation	KIRSYPVTEQRVKHT CCCCCCCCHHHCCCC	23.15	21712546
338	Phosphorylation	TKPRTYPSLLQGEYN CCCCCCHHHHCCCCC	31.16	20860994
344	Phosphorylation	PSLLQGEYNNRHPNI HHHHCCCCCCCCCCC	25.27	24719451
347	Ubiquitination	LQGEYNNRHPNIKQD HCCCCCCCCCCCCCC	42.90	29967540
352	Ubiquitination	NNRHPNIKQDQKSSQ CCCCCCCCCCHHHCC	55.23	29967540
382	Phosphorylation	ISQRPNLTVGRFNSA HHCCCCCEEEECCCC	27.79	-
388	Phosphorylation	LTVGRFNSAIPSTPS CEEEECCCCCCCCCC	25.51	27732954
391	Ubiquitination	GRFNSAIPSTPSIRP EECCCCCCCCCCCCC	31.69	29967540
392	Phosphorylation	RFNSAIPSTPSIRPN ECCCCCCCCCCCCCC	47.00	20068231
393	Phosphorylation	FNSAIPSTPSIRPNG CCCCCCCCCCCCCCC	18.72	27732954
395	Phosphorylation	SAIPSTPSIRPNGTS CCCCCCCCCCCCCCC	31.17	24719451
396	Ubiquitination	AIPSTPSIRPNGTSG CCCCCCCCCCCCCCC	10.00	-
401	Phosphorylation	PSIRPNGTSGNKHNN CCCCCCCCCCCCCCC	39.85	28348404
402	Phosphorylation	SIRPNGTSGNKHNNN CCCCCCCCCCCCCCC	41.59	20068231
417	Ubiquitination	GFQQKAQTLDSKLKK HHHHHHHHHHHHHHH	37.07	-
417	Ubiquitination	GFQQKAQTLDSKLKK HHHHHHHHHHHHHHH	37.07	29967540
420	Phosphorylation	QKAQTLDSKLKKAVP HHHHHHHHHHHHHHC	42.89	20860994
424	Ubiquitination	TLDSKLKKAVPQNHF HHHHHHHHHHCCCCC	66.12	29967540
428	Ubiquitination	KLKKAVPQNHFLNKT HHHHHHCCCCCCCCC	49.81	-
428	Ubiquitination	KLKKAVPQNHFLNKT HHHHHHCCCCCCCCC	49.81	29967540
434	Ubiquitination	PQNHFLNKTAPKTQA CCCCCCCCCCCCCCC	48.49	29967540
435	Phosphorylation	QNHFLNKTAPKTQAD CCCCCCCCCCCCCCC	47.31	27690223
438	Ubiquitination	FLNKTAPKTQADVTT CCCCCCCCCCCCEEE	51.29	-
444	Phosphorylation	PKTQADVTTVNGTQT CCCCCCEEEECCCCC	26.10	-
449	Phosphorylation	DVTTVNGTQTNPNIK CEEEECCCCCCCCHH	27.32	20860994
451	Phosphorylation	TTVNGTQTNPNIKKK EEECCCCCCCCHHHC	53.10	20860994
456	Acetylation	TQTNPNIKKKATAED CCCCCCHHHCCCHHH	55.66	19817381
457	Acetylation	QTNPNIKKKATAEDR CCCCCHHHCCCHHHH	43.79	19817389
457	Ubiquitination	QTNPNIKKKATAEDR CCCCCHHHCCCHHHH	43.79	-
466	Ubiquitination	ATAEDRRKQLEEWQK CCHHHHHHHHHHHHH	61.19	29967540
469	Ubiquitination	EDRRKQLEEWQKSKG HHHHHHHHHHHHHCC	55.48	-
469	Ubiquitination	EDRRKQLEEWQKSKG HHHHHHHHHHHHHCC	55.48	29967540
473	Ubiquitination	KQLEEWQKSKGKTYK HHHHHHHHHCCCCCC	56.03	29967540
474	Phosphorylation	QLEEWQKSKGKTYKR HHHHHHHHCCCCCCC	31.38	20860994
475	Ubiquitination	LEEWQKSKGKTYKRP HHHHHHHCCCCCCCC	72.03	29967540
479	Phosphorylation	QKSKGKTYKRPPMEL HHHCCCCCCCCCCCH	14.42	-
480	Ubiquitination	KSKGKTYKRPPMELK HHCCCCCCCCCCCHH	64.76	29967540
488	Ubiquitination	RPPMELKTKRKVIKE CCCCCHHHHHHHHHH	49.02	-
488	Ubiquitination	RPPMELKTKRKVIKE CCCCCHHHHHHHHHH	49.02	29967540
494	2-Hydroxyisobutyrylation	KTKRKVIKEMNISFW HHHHHHHHHHCHHHH	54.63	-
498	Ubiquitination	KVIKEMNISFWKSIE HHHHHHCHHHHHHCC	3.29	-
498	Ubiquitination	KVIKEMNISFWKSIE HHHHHHCHHHHHHCC	3.29	29967540
499	Phosphorylation	VIKEMNISFWKSIEK HHHHHCHHHHHHCCH	21.76	22817900
503	Phosphorylation	MNISFWKSIEKEEEE HCHHHHHHCCHHHHH	26.95	20860994
512	Ubiquitination	EKEEEEKKAQLELSS CHHHHHHHHHHHHHH	44.65	29967540
517	Ubiquitination	EKKAQLELSSKINNT HHHHHHHHHHHHHHH	10.74	-
556	Ubiquitination	PEAEKFAKFWICKAK CHHHHHHHHHHHHHH	44.35	29967540
561	Ubiquitination	FAKFWICKAKLLASK HHHHHHHHHHHHHCC	39.15	-
582	Ubiquitination	GLYEEAIKNGATPIQ HHHHHHHHCCCCHHH	57.27	29967540
586	Phosphorylation	EAIKNGATPIQELRK HHHHCCCCHHHHHHH	23.48	21712546
593	Ubiquitination	TPIQELRKVVLNILQ CHHHHHHHHHHHHHC	49.69	29967540
610	Phosphorylation	NRTTEGITSDSLVAE CCCCCCCCCCCHHHC	37.02	22210691
618	Phosphorylation	SDSLVAETSITSVEE CCCHHHCCCCCCHHH	18.72	22210691
619	Phosphorylation	DSLVAETSITSVEEL CCHHHCCCCCCHHHH	18.51	24719451
621	Phosphorylation	LVAETSITSVEELAK HHHCCCCCCHHHHHH	26.87	-
622	Phosphorylation	VAETSITSVEELAKK HHCCCCCCHHHHHHH	26.34	-
632	Phosphorylation	ELAKKMESVKSCLSP HHHHHHHHHHHHCCH	31.20	-
634	Ubiquitination	AKKMESVKSCLSPKE HHHHHHHHHHCCHHH	44.74	29967540
635	Phosphorylation	KKMESVKSCLSPKER HHHHHHHHHCCHHHH	20.38	26552605
638	Phosphorylation	ESVKSCLSPKEREQV HHHHHHCCHHHHHHH	37.95	30576142
640	Ubiquitination	VKSCLSPKEREQVTA HHHHCCHHHHHHHCC	67.06	29967540
646	Phosphorylation	PKEREQVTATPRIAK HHHHHHHCCCCCHHH	25.22	20860994
648	Phosphorylation	EREQVTATPRIAKAE HHHHHCCCCCHHHHH	12.07	25159151
653	Ubiquitination	TATPRIAKAEQHNYP CCCCCHHHHHHCCCC	50.51	29967540
659	Phosphorylation	AKAEQHNYPGIKLQI HHHHHCCCCCCEEEE	10.74	25884760
663	Ubiquitination	QHNYPGIKLQIGPIP HCCCCCCEEEECCCC	40.01	29967540
682	Ubiquitination	MPEVQDMKFITPVRR CCCCCCCEEECCCCC	41.74	-
685	Phosphorylation	VQDMKFITPVRRSSR CCCCEEECCCCCHHH	20.90	29496963
690	Phosphorylation	FITPVRRSSRIERAV EECCCCCHHHHHHHH	17.26	27174698
691	Phosphorylation	ITPVRRSSRIERAVS ECCCCCHHHHHHHHH	35.15	27174698
737	Phosphorylation	RNEALPVTLGFQTPE CCCCCCEEECCCCCC	21.15	20068231
742	Phosphorylation	PVTLGFQTPES---- CEEECCCCCCC----	27.11	30266825
745	Phosphorylation	LGFQTPES------- ECCCCCCC-------	46.88	30266825

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of CKP2L_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CKP2L_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CKP2L_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of CKP2L_HUMAN !!

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
272440	Filippi syndrome (FLPIS)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CKP2L_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-742 AND SER-745, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Phosphoproteome analysis of the human mitotic spindle."; Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.; Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499; THR-648; THR-685AND THR-742, AND MASS SPECTROMETRY.	16565220 [Abstract]
"In vivo identification of sumoylation sites by a signature tag andcysteine-targeted affinity purification."; Blomster H.A., Imanishi S.Y., Siimes J., Kastu J., Morrice N.A.,Eriksson J.E., Sistonen L.; J. Biol. Chem. 285:19324-19329(2010). Cited for: SUMOYLATION AT LYS-198, PHOSPHORYLATION AT TYR-204, AND MUTAGENESIS OFLYS-198.	20388717 [Abstract]