CHAP1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CHAP1_MOUSE
• UniProt AC: Q8K327
• Protein Name: Chromosome alignment-maintaining phosphoprotein 1
• Gene Name: Champ1
• Organism: Mus musculus (Mouse).
• Sequence Length: 802
• Subcellular Localization: Nucleus. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.
• Protein Description: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore (By similarity)..
• Protein Sequence: MEVCQELRKPALSLECGHCSFRGTDYENVQLHMGSIHPEFCDDMDAGGLGKLIFYQKSAKLFHCHKCFFTSKLYANVYYHITARHAASDKWSEQPKEQPSKDTESGKSPSPPERQNPAFDPAEARPTPALPMEAQKTSPSLCPESQASGPPVLEPQGAGPLISPEPQAPCLPAEASKAAPVPCPERVDPPCELPELEKPERGPSPESVKSALVSSKPPKHSSFADTGAAPSALSPESPVLATSPEPWGPSLSASPESRKPARTASPEPRKPSPAESPELWKPFPAIASEPRRPTPAVSPGSWKPGPPGSPRPWKSSPSATSGPWKSSKPVQPMSPGPWKPIPSVSPGPWKPAPSMSTASWKSSVSSGSWKTPPTSPESWKSGPPELRKTALPLSPEHWKAVPPVSPELRRPGPPLSPEIRSPAGSPELKKPSSSPDLWKVSPDQRKTSPASLDFPEPQKSSCGSPPDLWKSSFIMESQKPNVFSETRKHTASGSSESPKVASDIWKPVLSIDAEPRKSTLFPEPTKAVLPASPEPRKRALFPESRKHVFLPELPKSAVFSDAQKAPELSEEIQLEAVDNAKCDSLAQEGLLATPKKLLDEALSPSSKKLKKDSQENSDAELSSSEYIRADLDTLDTKGQESSSDQEQVDVESIDFSKENKMEMGSTEQAKNVLQFTEEKEAFISEEEIAKYMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCTICGKAFLLESLLKNHVAAHGQSLLKCPRCNFESNFPRGFKKHLTHCQSRHNEEVNKKLMEALESPLEEQQI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEVCQELR -------CHHHHHHH	8.65	-
100	Phosphorylation	EQPKEQPSKDTESGK CCCCCCCCCCCCCCC	42.74	25159016
103	Phosphorylation	KEQPSKDTESGKSPS CCCCCCCCCCCCCCC	35.47	25159016
105	Phosphorylation	QPSKDTESGKSPSPP CCCCCCCCCCCCCCC	54.32	25521595
108	Phosphorylation	KDTESGKSPSPPERQ CCCCCCCCCCCCHHC	33.85	26824392
110	Phosphorylation	TESGKSPSPPERQNP CCCCCCCCCCHHCCC	61.24	26824392
137	Phosphorylation	LPMEAQKTSPSLCPE CCCCCCCCCCCCCCH	33.52	27087446
138	Phosphorylation	PMEAQKTSPSLCPES CCCCCCCCCCCCCHH	21.37	25293948
140	Phosphorylation	EAQKTSPSLCPESQA CCCCCCCCCCCHHHC	42.23	27087446
145	Phosphorylation	SPSLCPESQASGPPV CCCCCCHHHCCCCCC	19.05	25195567
148	Phosphorylation	LCPESQASGPPVLEP CCCHHHCCCCCCCCC	44.17	25293948
163	Phosphorylation	QGAGPLISPEPQAPC CCCCCCCCCCCCCCC	30.78	25777480
176	Phosphorylation	PCLPAEASKAAPVPC CCCCHHHHHCCCCCC	17.84	25777480
204	Phosphorylation	EKPERGPSPESVKSA CCCCCCCCHHHHHHH	44.09	26824392
207	Phosphorylation	ERGPSPESVKSALVS CCCCCHHHHHHHHHC	37.92	28833060
215	Phosphorylation	VKSALVSSKPPKHSS HHHHHHCCCCCCCCC	41.52	24719451
221	Phosphorylation	SSKPPKHSSFADTGA CCCCCCCCCCCCCCC	33.02	25619855
222	Phosphorylation	SKPPKHSSFADTGAA CCCCCCCCCCCCCCC	24.95	25619855
226	Phosphorylation	KHSSFADTGAAPSAL CCCCCCCCCCCCCCC	25.87	25619855
231	Phosphorylation	ADTGAAPSALSPESP CCCCCCCCCCCCCCC	36.80	25619855
234	Phosphorylation	GAAPSALSPESPVLA CCCCCCCCCCCCCCC	26.74	21082442
237	Phosphorylation	PSALSPESPVLATSP CCCCCCCCCCCCCCC	24.56	21082442
242	Phosphorylation	PESPVLATSPEPWGP CCCCCCCCCCCCCCC	40.42	21082442
243	Phosphorylation	ESPVLATSPEPWGPS CCCCCCCCCCCCCCC	22.97	22942356
250	Phosphorylation	SPEPWGPSLSASPES CCCCCCCCCCCCHHH	30.90	21082442
252	Phosphorylation	EPWGPSLSASPESRK CCCCCCCCCCHHHCC	30.91	21082442
254	Phosphorylation	WGPSLSASPESRKPA CCCCCCCCHHHCCCC	26.31	21082442
257	Phosphorylation	SLSASPESRKPARTA CCCCCHHHCCCCCCC	49.33	21082442
263	Phosphorylation	ESRKPARTASPEPRK HHCCCCCCCCCCCCC	33.33	27087446
265	Phosphorylation	RKPARTASPEPRKPS CCCCCCCCCCCCCCC	29.23	27087446
272	Phosphorylation	SPEPRKPSPAESPEL CCCCCCCCCCCCHHH	39.51	26824392
276	Phosphorylation	RKPSPAESPELWKPF CCCCCCCCHHHHCCC	26.27	26824392
288	Phosphorylation	KPFPAIASEPRRPTP CCCCCCCCCCCCCCC	41.86	25159016
294	Phosphorylation	ASEPRRPTPAVSPGS CCCCCCCCCCCCCCC	23.28	29233185
298	Phosphorylation	RRPTPAVSPGSWKPG CCCCCCCCCCCCCCC	26.16	23684622
301	Phosphorylation	TPAVSPGSWKPGPPG CCCCCCCCCCCCCCC	35.00	26643407
309	Phosphorylation	WKPGPPGSPRPWKSS CCCCCCCCCCCCCCC	24.41	21082442
315	Phosphorylation	GSPRPWKSSPSATSG CCCCCCCCCCCCCCC	43.54	25266776
316	Phosphorylation	SPRPWKSSPSATSGP CCCCCCCCCCCCCCC	21.10	25266776
318	Phosphorylation	RPWKSSPSATSGPWK CCCCCCCCCCCCCCC	46.24	28066266
320	Phosphorylation	WKSSPSATSGPWKSS CCCCCCCCCCCCCCC	38.26	-
326	Phosphorylation	ATSGPWKSSKPVQPM CCCCCCCCCCCCCCC	39.46	26745281
327	Phosphorylation	TSGPWKSSKPVQPMS CCCCCCCCCCCCCCC	36.93	26745281
334	Phosphorylation	SKPVQPMSPGPWKPI CCCCCCCCCCCCCCC	33.13	25266776
343	Phosphorylation	GPWKPIPSVSPGPWK CCCCCCCCCCCCCCC	35.63	26745281
345	Phosphorylation	WKPIPSVSPGPWKPA CCCCCCCCCCCCCCC	28.60	25266776
362	Phosphorylation	MSTASWKSSVSSGSW CCCCCCCCCCCCCCC	29.70	25159016
363	Phosphorylation	STASWKSSVSSGSWK CCCCCCCCCCCCCCC	23.45	25159016
365	Phosphorylation	ASWKSSVSSGSWKTP CCCCCCCCCCCCCCC	30.81	23984901
366	Phosphorylation	SWKSSVSSGSWKTPP CCCCCCCCCCCCCCC	34.39	25159016
368	Phosphorylation	KSSVSSGSWKTPPTS CCCCCCCCCCCCCCC	27.61	26239621
371	Phosphorylation	VSSGSWKTPPTSPES CCCCCCCCCCCCHHH	28.39	23684622
374	Phosphorylation	GSWKTPPTSPESWKS CCCCCCCCCHHHHHC	59.11	23684622
375	Phosphorylation	SWKTPPTSPESWKSG CCCCCCCCHHHHHCC	32.23	22942356
378	Phosphorylation	TPPTSPESWKSGPPE CCCCCHHHHHCCCHH	43.09	25619855
389	Phosphorylation	GPPELRKTALPLSPE CCHHHHHCCCCCCHH	27.30	28833060
394	Phosphorylation	RKTALPLSPEHWKAV HHCCCCCCHHHHHCC	26.71	26824392
405	Phosphorylation	WKAVPPVSPELRRPG HHCCCCCCHHHCCCC	20.59	23527152
416	Phosphorylation	RRPGPPLSPEIRSPA CCCCCCCCHHHCCCC	26.68	27087446
421	Phosphorylation	PLSPEIRSPAGSPEL CCCHHHCCCCCCCCC	25.43	27087446
425	Phosphorylation	EIRSPAGSPELKKPS HHCCCCCCCCCCCCC	19.80	27087446
432	Phosphorylation	SPELKKPSSSPDLWK CCCCCCCCCCCCCCC	52.94	26824392
433	Phosphorylation	PELKKPSSSPDLWKV CCCCCCCCCCCCCCC	55.18	25521595
434	Phosphorylation	ELKKPSSSPDLWKVS CCCCCCCCCCCCCCC	27.11	25521595
441	Phosphorylation	SPDLWKVSPDQRKTS CCCCCCCCCCCCCCC	21.02	27149854
447	Phosphorylation	VSPDQRKTSPASLDF CCCCCCCCCCCCCCC	41.97	25521595
448	Phosphorylation	SPDQRKTSPASLDFP CCCCCCCCCCCCCCC	22.85	27087446
451	Phosphorylation	QRKTSPASLDFPEPQ CCCCCCCCCCCCCCC	31.68	27742792
460	Phosphorylation	DFPEPQKSSCGSPPD CCCCCCCCCCCCCHH	26.13	25619855
461	Phosphorylation	FPEPQKSSCGSPPDL CCCCCCCCCCCCHHH	29.54	25619855
464	Phosphorylation	PQKSSCGSPPDLWKS CCCCCCCCCHHHHHH	37.27	27087446
479	Acetylation	SFIMESQKPNVFSET HHHHHCCCCCCCCCC	48.08	-
490	Phosphorylation	FSETRKHTASGSSES CCCCCCCCCCCCCCC	26.08	21183079
492	Phosphorylation	ETRKHTASGSSESPK CCCCCCCCCCCCCCC	40.12	25266776
494	Phosphorylation	RKHTASGSSESPKVA CCCCCCCCCCCCCHH	28.56	19854140
497	Phosphorylation	TASGSSESPKVASDI CCCCCCCCCCHHHHH	31.78	22942356
502	Phosphorylation	SESPKVASDIWKPVL CCCCCHHHHHCCCCE	32.39	-
506	Acetylation	KVASDIWKPVLSIDA CHHHHHCCCCEECCC	26.11	23236377
532	Phosphorylation	TKAVLPASPEPRKRA CCCCCCCCCCHHHHC	28.05	27087446
546	Acetylation	ALFPESRKHVFLPEL CCCCCCCCCCCCCCC	54.09	23806337
593	Phosphorylation	AQEGLLATPKKLLDE HHCCCCCCHHHHHHH	35.29	26824392
603	Phosphorylation	KLLDEALSPSSKKLK HHHHHHHCCCHHHHH	29.77	27087446
605	Phosphorylation	LDEALSPSSKKLKKD HHHHHCCCHHHHHHH	51.95	22942356
606	Phosphorylation	DEALSPSSKKLKKDS HHHHCCCHHHHHHHC	36.87	22942356
607	Acetylation	EALSPSSKKLKKDSQ HHHCCCHHHHHHHCC	67.47	90837
613	Phosphorylation	SKKLKKDSQENSDAE HHHHHHHCCCCCCCC	48.55	25521595
617	Phosphorylation	KKDSQENSDAELSSS HHHCCCCCCCCCCHH	37.13	25521595
622	Phosphorylation	ENSDAELSSSEYIRA CCCCCCCCHHHHHHH	24.27	27087446
623	Phosphorylation	NSDAELSSSEYIRAD CCCCCCCHHHHHHHH	39.55	27087446
624	Phosphorylation	SDAELSSSEYIRADL CCCCCCHHHHHHHHH	30.92	25619855
626	Phosphorylation	AELSSSEYIRADLDT CCCCHHHHHHHHHHH	9.54	25619855
633	Phosphorylation	YIRADLDTLDTKGQE HHHHHHHHCCCCCCC	33.55	29899451
636	Phosphorylation	ADLDTLDTKGQESSS HHHHHCCCCCCCCCC	39.94	30635358
641	Phosphorylation	LDTKGQESSSDQEQV CCCCCCCCCCCHHHC	27.53	27087446
642	Phosphorylation	DTKGQESSSDQEQVD CCCCCCCCCCHHHCC	36.86	27087446
643	Phosphorylation	TKGQESSSDQEQVDV CCCCCCCCCHHHCCH	53.22	27087446
652	Phosphorylation	QEQVDVESIDFSKEN HHHCCHHHCCCCHHH	27.57	25619855
656	Phosphorylation	DVESIDFSKENKMEM CHHHCCCCHHHCCCC	35.00	25619855
657	Ubiquitination	VESIDFSKENKMEMG HHHCCCCHHHCCCCC	66.36	-
665	Phosphorylation	ENKMEMGSTEQAKNV HHCCCCCCHHHHHHH	27.71	-
726	Phosphorylation	VNKHNVHSPYKCTIC HHHCCCCCCCCCCCC	26.16	27149854
795	Phosphorylation	KLMEALESPLEEQQI HHHHHHHCCHHHHCC	35.32	21149613

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CHAP1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CHAP1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CHAP1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of CHAP1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-603, AND MASSSPECTROMETRY.
PubMed: 19131326

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-416 AND SER-532, ANDMASS SPECTROMETRY.
PubMed: 19144319

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-643, AND MASSSPECTROMETRY.
PubMed: 19367708

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309, AND MASSSPECTROMETRY.
PubMed: 17242355