CDT1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CDT1_MOUSE
• UniProt AC: Q8R4E9
• Protein Name: DNA replication factor Cdt1
• Gene Name: Cdt1 {ECO:0000303|PubMed:11850834}
• Organism: Mus musculus (Mouse).
• Sequence Length: 557
• Subcellular Localization: Nucleus . Chromosome, centromere, kinetochore . Transiently localizes to kinetochores during prometaphase and metaphase.
• Protein Description: Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments (By similarity). Potential oncogene. [PubMed: 11850834]
• Protein Sequence: MAQSRVTDFYACRRPGLTTPRAKSICLTPSPGGLVAPAFTRSSSRKRARPPAEPGSDQPAPLARRRLRLPGLDSCPSSLPEPSSPAEPSPPADPSPPADPGSPVCPSPVKRTKSTTVYVGQQPGKIPSEDSVSELQSCLRRARKLGAQARALRARVQENAVEPSTPDAKVPTEQPCVEKAPAYQRFHALAQPGLPGLVLPYKYQVLVEMFRSMDTIVSMLHNRSETVTFAKVKQGVQEMMRKRFEERNVGQIKTVYPTSYRFRQECNVPTFKDSIKRSDYQLTIEPLLGQEAGGATQLTATCLLQRRQVFRQNLVERVKEQHKVFLASLNPPMAVPDDQLTRWHPRFNVDEVPDIEPAELPQPPVTEKLTTAQEVLARARSLMTPKMEKALSNLALRSAEPGSPGTSTPPLPATPPATPPAASPSALKGVSQALLERIRAKEVQKQLARMTRCPEQELRLQRLERLPELARVLRNVFVSERKPALTMEVVCARMVDSCQTALSPGEMEKHLVLLAELLPDWLSLHRIRTDTYVKLDKAVDLAGLTARLAHHVHAEGL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Phosphorylation	ACRRPGLTTPRAKSI CCCCCCCCCCCCCEE	40.44	23984901
19	Phosphorylation	CRRPGLTTPRAKSIC CCCCCCCCCCCCEEE	18.80	23984901
23	Ubiquitination	GLTTPRAKSICLTPS CCCCCCCCEEEECCC	41.45	-
24	Phosphorylation	LTTPRAKSICLTPSP CCCCCCCEEEECCCC	19.97	27600695
28	Phosphorylation	RAKSICLTPSPGGLV CCCEEEECCCCCCCC	19.80	26745281
30	Phosphorylation	KSICLTPSPGGLVAP CEEEECCCCCCCCCC	30.83	26745281
102	Phosphorylation	SPPADPGSPVCPSPV CCCCCCCCCCCCCCC	21.39	25338131
107	Phosphorylation	PGSPVCPSPVKRTKS CCCCCCCCCCCCCCC	36.88	-
114	Phosphorylation	SPVKRTKSTTVYVGQ CCCCCCCCEEEEECC	28.79	25338131
115	Phosphorylation	PVKRTKSTTVYVGQQ CCCCCCCEEEEECCC	23.10	28285833
164	Phosphorylation	QENAVEPSTPDAKVP HHCCCCCCCCCCCCC	38.78	25266776
165	Phosphorylation	ENAVEPSTPDAKVPT HCCCCCCCCCCCCCC	35.58	25266776
392	Phosphorylation	PKMEKALSNLALRSA HHHHHHHHHHHHHCC	34.36	26643407
398	Phosphorylation	LSNLALRSAEPGSPG HHHHHHHCCCCCCCC	37.52	25777480
403	Phosphorylation	LRSAEPGSPGTSTPP HHCCCCCCCCCCCCC	30.76	21659605
406	Phosphorylation	AEPGSPGTSTPPLPA CCCCCCCCCCCCCCC	32.58	26643407
407	Phosphorylation	EPGSPGTSTPPLPAT CCCCCCCCCCCCCCC	44.85	25777480
408	Phosphorylation	PGSPGTSTPPLPATP CCCCCCCCCCCCCCC	28.85	25777480
414	Phosphorylation	STPPLPATPPATPPA CCCCCCCCCCCCCCC	28.06	21659605
418	Phosphorylation	LPATPPATPPAASPS CCCCCCCCCCCCCHH	35.26	25777480
423	Phosphorylation	PATPPAASPSALKGV CCCCCCCCHHHHHHH	22.84	25777480
425	Phosphorylation	TPPAASPSALKGVSQ CCCCCCHHHHHHHHH	43.68	25777480

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
28	T	Phosphorylation	Kinase	MAPK8	Q91Y86	Uniprot
107	S	Phosphorylation	Kinase	MAPK8	Q91Y86	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
28	T	Phosphorylation	Phosphorylated at Thr-28 by MAPK8/JNK1, which blocks replication licensing in response to stress.	14993212
28	T	Phosphorylation	Phosphorylation at Thr-28 by CDK2 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation.	-
28	T	Phosphorylation	Phosphorylation by cyclin A-dependent kinases at Thr-28 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation.	14993212
28	T	ubiquitylation	Phosphorylation at Thr-28 by CDK2 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation.	-
28	T	ubiquitylation	Phosphorylation by cyclin A-dependent kinases at Thr-28 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation.	14993212

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CDT1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MCM6_YEAST	MCM6	physical	20335175
MCM6_MOUSE	Mcm6	physical	15286659

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.