CDPK8_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CDPK8_ARATH
• UniProt AC: Q42438
• Protein Name: Calcium-dependent protein kinase 8
• Gene Name: CPK8
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 533
• Subcellular Localization: Cell membrane ; Lipid-anchor .
• Protein Description: May play a role in signal transduction pathways that involve calcium as a second messenger..
• Protein Sequence: MGNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKDPTGHDISLMYDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDPKKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEVAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGEFVAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDVDTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMREGSLQLEGEN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGNCCASPG ------CCCCCCCCC	35.56	-
23	Phosphorylation	KGKPKIKSNPFYSEA CCCCCCCCCCCCCEE	53.89	29797451
28	Phosphorylation	IKSNPFYSEAYTTNG CCCCCCCCEEEEECC	19.01	19880383
31	Phosphorylation	NPFYSEAYTTNGSGT CCCCCEEEEECCCCC	15.28	23110452
32	Phosphorylation	PFYSEAYTTNGSGTG CCCCEEEEECCCCCC	22.34	19880383
33	Phosphorylation	FYSEAYTTNGSGTGF CCCEEEEECCCCCCE	25.55	20374526
36	Phosphorylation	EAYTTNGSGTGFKLS EEEEECCCCCCEEEE	34.94	30291188
38	Phosphorylation	YTTNGSGTGFKLSVL EEECCCCCCEEEEEE	40.94	15308754
221	Phosphorylation	GFNEIVGSPYYMAPE CHHHHCCCCCCCCHH	9.99	-
285	Phosphorylation	RDPWPRVSETAKDLV CCCCCCCCHHHHHHH	30.43	28011693
287	Phosphorylation	PWPRVSETAKDLVRK CCCCCCHHHHHHHHH	31.34	28011693
488	Phosphorylation	TDKDGRISYEEFAAM CCCCCCCCHHHHHHH	25.73	-
518	Phosphorylation	RERFNSLSLKLMREG HHHHHHHHHHHHHHC	24.23	23111157
526	Phosphorylation	LKLMREGSLQLEGEN HHHHHHCCCEEECCC	14.27	19376835

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CDPK8_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CDPK8_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CDPK8_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CATA3_ARATH	CAT3	physical	25966761

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale Arabidopsis phosphoproteome profiling reveals novelchloroplast kinase substrates and phosphorylation networks.";
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,Grossmann J., Gruissem W., Baginsky S.;
Plant Physiol. 150:889-903(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-526, AND MASSSPECTROMETRY.
PubMed: 19376835

"Phosphoproteomic analysis of nuclei-enriched fractions fromArabidopsis thaliana.";
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A.,Andreasson E., Rathjen J.P., Peck S.C.;
J. Proteomics 72:439-451(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, AND MASSSPECTROMETRY.
PubMed: 19245862

"Large-scale analysis of in vivo phosphorylated membrane proteins byimmobilized metal ion affinity chromatography and mass spectrometry.";
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
Mol. Cell. Proteomics 2:1234-1243(2003).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, AND MASSSPECTROMETRY.
PubMed: 14506206