dbPTM

CDK12_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CDK12_MOUSE
UniProt AC	Q14AX6
Protein Name	Cyclin-dependent kinase 12
Gene Name	Cdk12
Organism	Mus musculus (Mouse).
Sequence Length	1484
Subcellular Localization	Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase..
Protein Description	Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors..
Protein Sequence	MPNSERHGGKKDGSGGASGTSQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDVGLVTPEAASLGTIIKPLVEYDDISSDSDTFSDDTAFKSDRRENEERRGTDRSDRLHRHRHHQHRRSRDLLKTKQTEKEKNQEVSKSGSMKDRVSGSSKRSVEGSDDYGKAQLSKSGSKESRSSKMHKEKTRKERELKSGYKDRSKSHRKRETPKSYKTVASPKRRSRSPHRKWSDSSKQDDSPSGASYGQDYDLSPPRSHTSSNYDSYKKSPGSTSRRQSISPPYKEPSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLSRSPLPSRKSMKSRSRSPAYSRHSSSHSKKKRSGSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKMDGKESKSSPIILPKKEKLEVKESGLESKKLPRSIKSEKSTPDTELVTVAHSNPEVKHCLDTGKVRLDENLQKHPAKDLKAQGTKDVKPVAPKEVIVTSKETETSEKETLPPLPTITSPPPLPATTPPPQTPPLPPLPPLPAIPLQPPLPPPQPPFSQVPVSSTSILPSSPHPRTSTLSSQTNSQPPVQVSMKTQVSITAAIPHLKTSTLPPLPLPPLLPGDDDMDSPKETLPSKPAKKEKEQRTRHLLTDLPLPPELPGGDPSPPDSPEPKAITPPQQPYKKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLVHQSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLDYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSGIVIEDPPPSKASRKETTSGTTAEPVKNNSPAPPQPAPVKAEPGPGDAVGLGDITQQLNQSELAVLLNLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEASSQQQDSESIAPEESLKEVPSVPVVLPPAEQTTPEASNTPADMQNVLAVLLSQLMKTQEPAGNLEENTNDKNSGPQGPRRTPTMPQEEAAACPPHILPPEKRPPEPPGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRSHPNRTYGNTDGPETGFSSADTDERSSGPALTESLVQTPVKNRTFSGSVSHLGESNSYQGTGSVQFPGDQDLRFTRVPLALHSVVGQPFLKSEGNSNSVVHAETKLQNYGELGPGTTGANSSGTTLQWGGPAQSYGKPYRGAARVLPRGGRGRGVPY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Phosphorylation	HGGKKDGSGGASGTS CCCCCCCCCCCCCCC	44.16	25777480
18	Phosphorylation	KDGSGGASGTSQPSS CCCCCCCCCCCCCCC	45.97	25777480
20	Phosphorylation	GSGGASGTSQPSSGG CCCCCCCCCCCCCCC	22.99	23984901
21	Phosphorylation	SGGASGTSQPSSGGG CCCCCCCCCCCCCCC	43.55	23984901
24	Phosphorylation	ASGTSQPSSGGGSSN CCCCCCCCCCCCCCC	33.70	23684622
25	Phosphorylation	SGTSQPSSGGGSSNS CCCCCCCCCCCCCCH	48.41	23684622
29	Phosphorylation	QPSSGGGSSNSRERH CCCCCCCCCCHHHHH	29.62	23684622
30	Phosphorylation	PSSGGGSSNSRERHR CCCCCCCCCHHHHHH	41.94	23684622
32	Phosphorylation	SGGGSSNSRERHRLV CCCCCCCHHHHHHHH	37.15	24899341
57	Phosphorylation	SKDVGLVTPEAASLG CCCCCCCCHHHHHHC	22.06	26824392
62	Phosphorylation	LVTPEAASLGTIIKP CCCHHHHHHCHHCHH	34.09	21183079
65	Phosphorylation	PEAASLGTIIKPLVE HHHHHHCHHCHHCCC	25.48	21183079
73	Phosphorylation	IIKPLVEYDDISSDS HCHHCCCCCCCCCCC	16.07	21743459
77	Phosphorylation	LVEYDDISSDSDTFS CCCCCCCCCCCCCCC	34.78	21743459
78	Phosphorylation	VEYDDISSDSDTFSD CCCCCCCCCCCCCCC	41.28	25195567
80	Phosphorylation	YDDISSDSDTFSDDT CCCCCCCCCCCCCCC	40.26	21743459
82	Phosphorylation	DISSDSDTFSDDTAF CCCCCCCCCCCCCCC	29.05	21743459
84	Phosphorylation	SSDSDTFSDDTAFKS CCCCCCCCCCCCCHH	36.35	21743459
87	Phosphorylation	SDTFSDDTAFKSDRR CCCCCCCCCCHHHHH	38.70	25195567
119	Phosphorylation	RHHQHRRSRDLLKTK HHHHHHHHHHHHHHH	30.06	27149854
141	Phosphorylation	QEVSKSGSMKDRVSG HHHHCCCCCCCCCCC	29.74	30482847
153	Phosphorylation	VSGSSKRSVEGSDDY CCCCCCCCCCCCCCH	28.40	29550500
157	Phosphorylation	SKRSVEGSDDYGKAQ CCCCCCCCCCHHHHH	17.50	23684622
205	Phosphorylation	KSHRKRETPKSYKTV HHHCCCCCCCHHHCC	39.80	-
214	Phosphorylation	KSYKTVASPKRRSRS CHHHCCCCCCCCCCC	26.98	27149854
227	Phosphorylation	RSPHRKWSDSSKQDD CCCCCCCCCCCCCCC	30.56	21149613
229	Phosphorylation	PHRKWSDSSKQDDSP CCCCCCCCCCCCCCC	33.64	21149613
230	Phosphorylation	HRKWSDSSKQDDSPS CCCCCCCCCCCCCCC	39.05	21149613
235	Phosphorylation	DSSKQDDSPSGASYG CCCCCCCCCCCCCCC	29.29	21082442
237	Phosphorylation	SKQDDSPSGASYGQD CCCCCCCCCCCCCCC	51.44	21149613
240	Phosphorylation	DDSPSGASYGQDYDL CCCCCCCCCCCCCCC	33.06	21149613
241	Phosphorylation	DSPSGASYGQDYDLS CCCCCCCCCCCCCCC	20.48	21149613
245	Phosphorylation	GASYGQDYDLSPPRS CCCCCCCCCCCCCCC	15.76	21149613
248	Phosphorylation	YGQDYDLSPPRSHTS CCCCCCCCCCCCCCC	29.93	21082442
255	Phosphorylation	SPPRSHTSSNYDSYK CCCCCCCCCCHHHCC	15.91	27841257
258	Phosphorylation	RSHTSSNYDSYKKSP CCCCCCCHHHCCCCC	14.28	25777480
260	Phosphorylation	HTSSNYDSYKKSPGS CCCCCHHHCCCCCCC	28.61	25777480
261	Phosphorylation	TSSNYDSYKKSPGST CCCCHHHCCCCCCCC	21.38	25777480
264	Phosphorylation	NYDSYKKSPGSTSRR CHHHCCCCCCCCCCC	30.27	23375375
267	Phosphorylation	SYKKSPGSTSRRQSI HCCCCCCCCCCCCCC	26.96	23375375
268	Phosphorylation	YKKSPGSTSRRQSIS CCCCCCCCCCCCCCC	31.97	23375375
269	Phosphorylation	KKSPGSTSRRQSISP CCCCCCCCCCCCCCC	27.45	23375375
273	Phosphorylation	GSTSRRQSISPPYKE CCCCCCCCCCCCCCC	24.19	27087446
275	Phosphorylation	TSRRQSISPPYKEPS CCCCCCCCCCCCCCC	25.68	27087446
278	Phosphorylation	RQSISPPYKEPSAYQ CCCCCCCCCCCCHHC	31.22	25159016
282	Phosphorylation	SPPYKEPSAYQSSTR CCCCCCCCHHCCCCC	40.47	26643407
284	Phosphorylation	PYKEPSAYQSSTRSP CCCCCCHHCCCCCCC	17.17	26643407
286	Phosphorylation	KEPSAYQSSTRSPSP CCCCHHCCCCCCCCC	22.47	26643407
287	Phosphorylation	EPSAYQSSTRSPSPY CCCHHCCCCCCCCCC	16.59	26643407
288	Phosphorylation	PSAYQSSTRSPSPYS CCHHCCCCCCCCCCC	40.17	26643407
290	Phosphorylation	AYQSSTRSPSPYSRR HHCCCCCCCCCCCCC	29.80	25521595
292	Phosphorylation	QSSTRSPSPYSRRQR CCCCCCCCCCCCCCC	37.40	25521595
294	Phosphorylation	STRSPSPYSRRQRSV CCCCCCCCCCCCCCC	21.10	25521595
295	Phosphorylation	TRSPSPYSRRQRSVS CCCCCCCCCCCCCCC	25.11	25159016
300	Phosphorylation	PYSRRQRSVSPYSRR CCCCCCCCCCCCCCC	20.71	27087446
302	Phosphorylation	SRRQRSVSPYSRRRS CCCCCCCCCCCCCCC	21.26	27087446
304	Phosphorylation	RQRSVSPYSRRRSSS CCCCCCCCCCCCCCC	13.80	25159016
305	Phosphorylation	QRSVSPYSRRRSSSY CCCCCCCCCCCCCCC	23.77	25159016
309	Phosphorylation	SPYSRRRSSSYERSG CCCCCCCCCCCCCCC	23.09	23684622
310	Phosphorylation	PYSRRRSSSYERSGS CCCCCCCCCCCCCCC	34.82	23684622
311	Phosphorylation	YSRRRSSSYERSGSY CCCCCCCCCCCCCCC	32.06	23684622
312	Phosphorylation	SRRRSSSYERSGSYS CCCCCCCCCCCCCCC	19.80	25367039
315	Phosphorylation	RSSSYERSGSYSGRS CCCCCCCCCCCCCCC	21.83	25159016
317	Phosphorylation	SSYERSGSYSGRSPS CCCCCCCCCCCCCCC	19.85	23684622
318	Phosphorylation	SYERSGSYSGRSPSP CCCCCCCCCCCCCCC	20.76	21082442
319	Phosphorylation	YERSGSYSGRSPSPY CCCCCCCCCCCCCCC	29.45	23684622
322	Phosphorylation	SGSYSGRSPSPYGRR CCCCCCCCCCCCCCC	33.00	27087446
324	Phosphorylation	SYSGRSPSPYGRRRS CCCCCCCCCCCCCCC	31.81	27087446
326	Phosphorylation	SGRSPSPYGRRRSSS CCCCCCCCCCCCCCC	27.83	21082442
331	Phosphorylation	SPYGRRRSSSPFLSK CCCCCCCCCCCCCCC	33.10	27087446
332	Phosphorylation	PYGRRRSSSPFLSKR CCCCCCCCCCCCCCC	39.97	25521595
333	Phosphorylation	YGRRRSSSPFLSKRS CCCCCCCCCCCCCCC	22.40	25521595
337	Phosphorylation	RSSSPFLSKRSLSRS CCCCCCCCCCCCCCC	26.80	25168779
340	Phosphorylation	SPFLSKRSLSRSPLP CCCCCCCCCCCCCCC	34.14	28973931
342	Phosphorylation	FLSKRSLSRSPLPSR CCCCCCCCCCCCCCH	31.77	28973931
344	Phosphorylation	SKRSLSRSPLPSRKS CCCCCCCCCCCCHHH	27.67	28973931
348	Phosphorylation	LSRSPLPSRKSMKSR CCCCCCCCHHHHHCC	61.08	21743459
354	Phosphorylation	PSRKSMKSRSRSPAY CCHHHHHCCCCCCCH	27.18	22817900
356	Phosphorylation	RKSMKSRSRSPAYSR HHHHHCCCCCCCHHC	44.17	29899451
358	Phosphorylation	SMKSRSRSPAYSRHS HHHCCCCCCCHHCCC	18.35	22324799
361	Phosphorylation	SRSRSPAYSRHSSSH CCCCCCCHHCCCCCC	15.04	29550500
362	Phosphorylation	RSRSPAYSRHSSSHS CCCCCCHHCCCCCCC	26.14	29550500
376	Phosphorylation	SKKKRSGSRSRHSSI CCCCCCCCCCCCCCC	28.47	22871156
378	Phosphorylation	KKRSGSRSRHSSISP CCCCCCCCCCCCCCC	35.72	23984901
381	Phosphorylation	SGSRSRHSSISPVRL CCCCCCCCCCCCCCC	28.40	27087446
382	Phosphorylation	GSRSRHSSISPVRLP CCCCCCCCCCCCCCC	22.44	27087446
384	Phosphorylation	RSRHSSISPVRLPLN CCCCCCCCCCCCCCC	21.20	27087446
392	Phosphorylation	PVRLPLNSSLGAELS CCCCCCCCHHHHHHH	34.32	25168779
393	Phosphorylation	VRLPLNSSLGAELSR CCCCCCCHHHHHHHH	29.74	25168779
399	Phosphorylation	SSLGAELSRKKKERA CHHHHHHHHHHHHHH	33.29	25619855
419	Phosphorylation	AKMDGKESKSSPIIL HHHCCCCCCCCCCCC	41.13	29176673
421	Phosphorylation	MDGKESKSSPIILPK HCCCCCCCCCCCCCC	51.10	25521595
422	Phosphorylation	DGKESKSSPIILPKK CCCCCCCCCCCCCCH	24.69	25521595
450	Phosphorylation	KLPRSIKSEKSTPDT CCCCCCCCCCCCCCC	48.89	26643407
453	Phosphorylation	RSIKSEKSTPDTELV CCCCCCCCCCCCCEE	40.91	26643407
454	Phosphorylation	SIKSEKSTPDTELVT CCCCCCCCCCCCEEE	35.93	26643407
457	Phosphorylation	SEKSTPDTELVTVAH CCCCCCCCCEEEEEC	32.21	26643407
461	Phosphorylation	TPDTELVTVAHSNPE CCCCCEEEEECCCHH	25.30	26643407
511	Phosphorylation	APKEVIVTSKETETS CCCEEEEEECCCCCC	23.98	-
589	O-linked_Glycosylation	SSPHPRTSTLSSQTN CCCCCCCCCCCCCCC	28.73	51587865
589	Phosphorylation	SSPHPRTSTLSSQTN CCCCCCCCCCCCCCC	28.73	-
595	Phosphorylation	TSTLSSQTNSQPPVQ CCCCCCCCCCCCCEE	38.49	-
610	Phosphorylation	VSMKTQVSITAAIPH EEEEEEEEEEEECCC	12.38	25266776
620	Phosphorylation	AAIPHLKTSTLPPLP EECCCCCCCCCCCCC	33.05	-
621	Phosphorylation	AIPHLKTSTLPPLPL ECCCCCCCCCCCCCC	26.76	-
622	Phosphorylation	IPHLKTSTLPPLPLP CCCCCCCCCCCCCCC	48.82	-
640	Phosphorylation	PGDDDMDSPKETLPS CCCCCCCCCHHCCCC	31.10	21082442
644	Phosphorylation	DMDSPKETLPSKPAK CCCCCHHCCCCCCCH	50.52	25266776
647	Phosphorylation	SPKETLPSKPAKKEK CCHHCCCCCCCHHHH	55.15	26643407
663	Phosphorylation	QRTRHLLTDLPLPPE HHHHHHHCCCCCCCC	41.68	25619855
677	Phosphorylation	ELPGGDPSPPDSPEP CCCCCCCCCCCCCCC	54.53	27087446
681	Phosphorylation	GDPSPPDSPEPKAIT CCCCCCCCCCCCCCC	36.62	27087446
688	Phosphorylation	SPEPKAITPPQQPYK CCCCCCCCCCCCCCC	33.50	26824392
880	Phosphorylation	DFGLARLYNSEESRP HHHHHHCCCCCCCCC	15.87	28833060
882	Phosphorylation	GLARLYNSEESRPYT HHHHCCCCCCCCCCC	29.23	28833060
885	Phosphorylation	RLYNSEESRPYTNKV HCCCCCCCCCCCCCE	34.21	28833060
888	Phosphorylation	NSEESRPYTNKVITL CCCCCCCCCCCEEEE	23.10	28833060
889	Phosphorylation	SEESRPYTNKVITLW CCCCCCCCCCEEEEE	31.11	25521595
987	Phosphorylation	EEFSFIPSAALDLLD HHHCCCCHHHHHHHH	22.74	21183079
1006	Phosphorylation	LDPSKRCTAEQTLQS CCHHHCCCHHHHHCH	36.94	21183079
1022	Phosphorylation	FLKDVELSKMAPPDL HHHHHHHHHCCCCCC	13.59	23737553
1049	Phosphorylation	KRRRQRQSGIVIEDP HHHHHHHCCCEECCC	32.45	25338131
1066	Phosphorylation	SKASRKETTSGTTAE CHHCCCCCCCCCCCC	29.33	25619855
1067	Phosphorylation	KASRKETTSGTTAEP HHCCCCCCCCCCCCC	26.67	25619855
1068	Phosphorylation	ASRKETTSGTTAEPV HCCCCCCCCCCCCCC	41.38	25619855
1070	Phosphorylation	RKETTSGTTAEPVKN CCCCCCCCCCCCCCC	23.70	25619855
1071	Phosphorylation	KETTSGTTAEPVKNN CCCCCCCCCCCCCCC	32.33	25619855
1079	Phosphorylation	AEPVKNNSPAPPQPA CCCCCCCCCCCCCCC	31.65	27087446
1196	Phosphorylation	EQTTPEASNTPADMQ HHCCCCCCCCCHHHH	39.30	23649490
1232	Phosphorylation	ENTNDKNSGPQGPRR CCCCCCCCCCCCCCC	58.55	23984901
1232 (in isoform 3)	Phosphorylation	-	58.55	26643407
1240	Phosphorylation	GPQGPRRTPTMPQEE CCCCCCCCCCCCHHH	25.13	21082442
1240 (in isoform 2)	Phosphorylation	-	25.13	23684622
1240 (in isoform 3)	Phosphorylation	-	25.13	25521595
1242	Phosphorylation	QGPRRTPTMPQEEAA CCCCCCCCCCHHHHH	40.65	21082442
1242 (in isoform 3)	Phosphorylation	-	40.65	25159016
1359	O-linked_Glycosylation	RSSGPALTESLVQTP CCCCCCCCHHHHCCC	26.15	55411567
1359	Phosphorylation	RSSGPALTESLVQTP CCCCCCCCHHHHCCC	26.15	25619855
1361	Phosphorylation	SGPALTESLVQTPVK CCCCCCHHHHCCCCC	28.71	25619855
1365	Phosphorylation	LTESLVQTPVKNRTF CCHHHHCCCCCCCEE	23.67	26824392
1371	Phosphorylation	QTPVKNRTFSGSVSH CCCCCCCEECEECEE	31.39	25338131

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of CDK12_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
889	T	Phosphorylation		21183079
Phosphorylation at Thr-889 increases kinase activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CDK12_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of CDK12_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CDK12_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry."; Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.; Mol. Cell. Proteomics 8:904-912(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382 AND SER-384, ANDMASS SPECTROMETRY.	19131326 [Abstract]
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1079, AND MASSSPECTROMETRY.	19144319 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273; THR-889 ANDSER-1079, AND MASS SPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-278; TYR-318; SER-322;SER-324; SER-382; SER-384 AND THR-1071, AND MASS SPECTROMETRY.	17242355 [Abstract]

show