CARL1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CARL1_HUMAN
• UniProt AC: Q5VZK9
• Protein Name: F-actin-uncapping protein LRRC16A {ECO:0000305}
• Gene Name: CARMIL1 {ECO:0000303|PubMed:16054028, ECO:0000312|HGNC:HGNC:21581}
• Organism: Homo sapiens (Human).
• Sequence Length: 1371
• Subcellular Localization: Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Colocalized with heterodimeric capping protein (CP) and F-actin in lamellipodia but not with F-actin in stress fibers (PubMed:19846667). Colocalized with F-actin i
• Protein Description: Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments. [PubMed: 16054028 Plays a role in lamellipodial protrusion formations and cell migration]
• Protein Sequence: MTEESSDVPRELIESIKDVIGRKIKISVKKKVKLEVKGDKVENKVLVLTSCRAFLVTARIPTKLELTFSYLEIHGVVCSKSAQMIVETEKCSISMKMASPEDVSEVLAHIGTCLRKIFPGLSPVRIMKKVSMEPSERLASLQALWDSQTVAEQGPCGGFSQMYACVCDWLGFSYREEVQWDVDTIYLTQDTRELNLQDFSHLDHRDLIPIIAALEYNQWFTKLSSKDLKLSTDVCEQILRVVSRSNRLEELVLENAGLRTDFAQKLASALAHNPNSGLHTINLAGNPLEDRGVSSLSIQFAKLPKGLKHLNLSKTSLSPKGVNSLSQSLSANPLTASTLVHLDLSGNVLRGDDLSHMYNFLAQPNAIVHLDLSNTECSLDMVCGALLRGCLQYLAVLNLSRTVFSHRKGKEVPPSFKQFFSSSLALMHINLSGTKLSPEPLKALLLGLACNHNLKGVSLDLSNCELRSGGAQVLEGCIAEIHNITSLDISDNGLESDLSTLIVWLSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEESPLQSLSLADSKLKTEVTIIINALGSNTSLTKVDISGNGMGDMGAKMLAKALQINTKLRTVIWDKNNITAQGFQDIAVAMEKNYTLRFMPIPMYDASQALKTNPEKTEDALQKIENYLLRNHETRKYLQEQAYRLQQGIVTSTTQQMIDRICVKVQDHLNSLRNCGGDAIQEDLKSAERLMRDAKNSKTLLPNLYHVGGASWAGASGLLSSPIQETLESMAGEVTRVVDEQLKALLESMVDAAENLCPNVMKKAHIRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLTVASFLSDRIVDEILDALSHCHHKLADHFSRRGKTLPQQESLEIELAEEKPVKRSIITVEELTEIERLEDLDTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVPIHIEDPPFPSLRQEKRSSGFISELPSEEGKKLEHFTKLRPKRNKKQQPTQAAVCAANIVSQDGEQNGLMGRVDEGVDEFFTKKVTKMDSKKWSTRGSESHELNEGGDEKKKRDSRKSSGFLNLIKSRSKSERPPTILMTEEPSSPKGAVRSPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEIGKVERSDSKSSPQAGRRYGVQVMGSGLLAEMKAKQEKRAACAQKKLGNDAVSQDSSSPALSGVERSDGGGAVPKLHPGLPENRFGLGTPEKNTKAEPKAEAGSRSRSSSSTPTSPKPLLQSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPPVLKKVPSDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEYQEQKQRSSSKDGHQGSKSNDSGEEAEKEFIFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MTEESSDV -------CCCCCCHH		22814378
5	Phosphorylation	---MTEESSDVPREL ---CCCCCCHHCHHH		-
6	Phosphorylation	--MTEESSDVPRELI --CCCCCCHHCHHHH		-
17	Ubiquitination	RELIESIKDVIGRKI HHHHHHHHHHHCCCC		29967540
81	Phosphorylation	HGVVCSKSAQMIVET EEEEEECCCCEEEEE		-
92	Phosphorylation	IVETEKCSISMKMAS EEEECCCEEEEEECC		28509920
94	Phosphorylation	ETEKCSISMKMASPE EECCCEEEEEECCHH		24719451
96	Ubiquitination	EKCSISMKMASPEDV CCCEEEEEECCHHHH		21890473
104	Phosphorylation	MASPEDVSEVLAHIG ECCHHHHHHHHHHHH		-
112	Phosphorylation	EVLAHIGTCLRKIFP HHHHHHHHHHHHHCC		-
116	Ubiquitination	HIGTCLRKIFPGLSP HHHHHHHHHCCCCCH		21890473
116	Ubiquitination	HIGTCLRKIFPGLSP HHHHHHHHHCCCCCH		21890473
116 (in isoform 1)	Ubiquitination	-		21890473
116 (in isoform 2)	Ubiquitination	-		21890473
116 (in isoform 3)	Ubiquitination	-		21890473
122	Phosphorylation	RKIFPGLSPVRIMKK HHHCCCCCHHHHHHC		28355574
186	Phosphorylation	QWDVDTIYLTQDTRE CCCCCEEEECCCCCC		22798277
280	Phosphorylation	NPNSGLHTINLAGNP CCCCCCCEEECCCCC		-
308	Ubiquitination	AKLPKGLKHLNLSKT ECCCCCCCCCCCCCC		29967540
314	Ubiquitination	LKHLNLSKTSLSPKG CCCCCCCCCCCCHHH		29967540
316	Phosphorylation	HLNLSKTSLSPKGVN CCCCCCCCCCHHHHH		24719451
318	Phosphorylation	NLSKTSLSPKGVNSL CCCCCCCCHHHHHHH		24719451
415	Phosphorylation	KGKEVPPSFKQFFSS CCCCCCCHHHHHHCH		-
462	Phosphorylation	KGVSLDLSNCELRSG CCCEECCCCCEECCC		22798277
573	Ubiquitination	ALGSNTSLTKVDISG CCCCCCCCEEEECCC		22817900
577	Ubiquitination	NTSLTKVDISGNGMG CCCCEEEECCCCCCH		22817900
579	Phosphorylation	SLTKVDISGNGMGDM CCEEEECCCCCCHHH		-
589	Ubiquitination	GMGDMGAKMLAKALQ CCHHHHHHHHHHHHH		22817900
593	Ubiquitination	MGAKMLAKALQINTK HHHHHHHHHHHHCCC		22817900
597	Ubiquitination	MLAKALQINTKLRTV HHHHHHHHCCCCCEE		22817900
599	Phosphorylation	AKALQINTKLRTVIW HHHHHHCCCCCEEEE		30576142
600	Ubiquitination	KALQINTKLRTVIWD HHHHHCCCCCEEEEC		32015554
628	Phosphorylation	VAMEKNYTLRFMPIP HHHHCCCEEEEEECC		24719451
640	Phosphorylation	PIPMYDASQALKTNP ECCCCCHHHHHHHCH		30631047
656	Ubiquitination	KTEDALQKIENYLLR HHHHHHHHHHHHHHH		29967540
660	Phosphorylation	ALQKIENYLLRNHET HHHHHHHHHHHCHHH		20393185
669	Methylation	LRNHETRKYLQEQAY HHCHHHHHHHHHHHH		115972607
676	Phosphorylation	KYLQEQAYRLQQGIV HHHHHHHHHHHCCCC		27642862
697	Ubiquitination	MIDRICVKVQDHLNS HHHHHHHHHHHHHHH		29967540
718	Ubiquitination	DAIQEDLKSAERLMR HHHHHHHHHHHHHHH		32015554
719	Phosphorylation	AIQEDLKSAERLMRD HHHHHHHHHHHHHHH		28102081
807	Phosphorylation	RQDLIHASTEKISIP HHHHHHHCCCCCCCC		30576142
810	Ubiquitination	LIHASTEKISIPRTF HHHHCCCCCCCCHHH		29967540
812	Phosphorylation	HASTEKISIPRTFVK HHCCCCCCCCHHHHH		24719451
819	Ubiquitination	SIPRTFVKNVLLEQS CCCHHHHHHHHHHHH		29967540
833	Ubiquitination	SGIDILNKISEVKLT HCHHHHHHHHCCHHH		29967540
835	Phosphorylation	IDILNKISEVKLTVA HHHHHHHHCCHHHHH		-
869	Phosphorylation	HKLADHFSRRGKTLP HHHHHHHHHCCCCCC		20068231
873	Ubiquitination	DHFSRRGKTLPQQES HHHHHCCCCCCHHHC		29967540
874	Phosphorylation	HFSRRGKTLPQQESL HHHHCCCCCCHHHCC		30108239
880	Phosphorylation	KTLPQQESLEIELAE CCCCHHHCCEEEECC		28355574
892	Ubiquitination	LAEEKPVKRSIITVE ECCCCCCCCEEEEHH		29967540
894	Phosphorylation	EEKPVKRSIITVEEL CCCCCCCEEEEHHHH		28348404
902	Phosphorylation	IITVEELTEIERLED EEEHHHHCHHHCHHH		30108239
912	Phosphorylation	ERLEDLDTCMMTPKS HCHHHCCCCCCCCHH		30266825
916	Phosphorylation	DLDTCMMTPKSKRKS HCCCCCCCCHHHHHC		19664994
919	Phosphorylation	TCMMTPKSKRKSIHS CCCCCCHHHHHCHHH		22210691
923	Phosphorylation	TPKSKRKSIHSRMLR CCHHHHHCHHHHCHH		20068231
926	Phosphorylation	SKRKSIHSRMLRPVS HHHHCHHHHCHHHHH		-
933	Phosphorylation	SRMLRPVSRAFEMEF HHCHHHHHHHHHEEE		26846344
960	Phosphorylation	IEDPPFPSLRQEKRS CCCCCCCCCCCHHHH		26657352
967	Phosphorylation	SLRQEKRSSGFISEL CCCCHHHHCCCCCCC		29255136
968	Phosphorylation	LRQEKRSSGFISELP CCCHHHHCCCCCCCC		19664994
972	Phosphorylation	KRSSGFISELPSEEG HHHCCCCCCCCCCCC		29255136
976	Phosphorylation	GFISELPSEEGKKLE CCCCCCCCCCCCCCH		23403867
986	Phosphorylation	GKKLEHFTKLRPKRN CCCCHHHHHCCCCCC		23312004
1036	Acetylation	FFTKKVTKMDSKKWS HHHHHHCCCCCCCCC		19820209
1039	Phosphorylation	KKVTKMDSKKWSTRG HHHCCCCCCCCCCCC		24719451
1043	Phosphorylation	KMDSKKWSTRGSESH CCCCCCCCCCCCCCC		25159151
1044	Phosphorylation	MDSKKWSTRGSESHE CCCCCCCCCCCCCCC		25159151
1047	Phosphorylation	KKWSTRGSESHELNE CCCCCCCCCCCCCCC		28857561
1049	Phosphorylation	WSTRGSESHELNEGG CCCCCCCCCCCCCCC		25159151
1064	Phosphorylation	DEKKKRDSRKSSGFL CHHHHHHHHHHHHHH		21712546
1067	Phosphorylation	KKRDSRKSSGFLNLI HHHHHHHHHHHHHHH		28355574
1068	Phosphorylation	KRDSRKSSGFLNLIK HHHHHHHHHHHHHHH		28355574
1076	Phosphorylation	GFLNLIKSRSKSERP HHHHHHHHCCCCCCC		24719451
1078	Phosphorylation	LNLIKSRSKSERPPT HHHHHHCCCCCCCCE		23186163
1080	Phosphorylation	LIKSRSKSERPPTIL HHHHCCCCCCCCEEE		23927012
1085	Phosphorylation	SKSERPPTILMTEEP CCCCCCCEEEEECCC		23403867
1089	Phosphorylation	RPPTILMTEEPSSPK CCCEEEEECCCCCCC		22167270
1093	Phosphorylation	ILMTEEPSSPKGAVR EEEECCCCCCCCCCC		29255136
1094	Phosphorylation	LMTEEPSSPKGAVRS EEECCCCCCCCCCCC		29255136
1101	Phosphorylation	SPKGAVRSPPVDCPR CCCCCCCCCCCCCCC		25159151
1128	Phosphorylation	ERIEEIKTPDSFEES HHHEECCCCCCCCHH		25159151
1131	Phosphorylation	EEIKTPDSFEESQGE EECCCCCCCCHHCCC		25159151
1135	Phosphorylation	TPDSFEESQGEEIGK CCCCCCHHCCCCCCC		30108239
1146	Phosphorylation	EIGKVERSDSKSSPQ CCCCCCCCCCCCCCC		30242111
1148	Phosphorylation	GKVERSDSKSSPQAG CCCCCCCCCCCCCCC		23312004
1150	Phosphorylation	VERSDSKSSPQAGRR CCCCCCCCCCCCCHH		25849741
1151	Phosphorylation	ERSDSKSSPQAGRRY CCCCCCCCCCCCHHH		25849741
1165	Phosphorylation	YGVQVMGSGLLAEMK HCEEEECCHHHHHHH		25159151
1183 (in isoform 2)	Phosphorylation	-		18669648
1192	Phosphorylation	KLGNDAVSQDSSSPA HHCCCCCCCCCCCCC		26356563
1195	Phosphorylation	NDAVSQDSSSPALSG CCCCCCCCCCCCHHC		26356563
1196	Phosphorylation	DAVSQDSSSPALSGV CCCCCCCCCCCHHCC		24300666
1197	Phosphorylation	AVSQDSSSPALSGVE CCCCCCCCCCHHCCC		26356563
1201	Phosphorylation	DSSSPALSGVERSDG CCCCCCHHCCCCCCC		23532336
1206	Phosphorylation	ALSGVERSDGGGAVP CHHCCCCCCCCCCCC		26356563
1228	Phosphorylation	ENRFGLGTPEKNTKA CCCCCCCCCCCCCCC		19664994
1233	Phosphorylation	LGTPEKNTKAEPKAE CCCCCCCCCCCCCCC		23186163
1243	Phosphorylation	EPKAEAGSRSRSSSS CCCCCCCCCCCCCCC		20068231
1245	Phosphorylation	KAEAGSRSRSSSSTP CCCCCCCCCCCCCCC		30576142
1247	Phosphorylation	EAGSRSRSSSSTPTS CCCCCCCCCCCCCCC		30108239
1248	Phosphorylation	AGSRSRSSSSTPTSP CCCCCCCCCCCCCCC		29496963
1249	Phosphorylation	GSRSRSSSSTPTSPK CCCCCCCCCCCCCCC		26657352
1250	Phosphorylation	SRSRSSSSTPTSPKP CCCCCCCCCCCCCCC		27794612
1251	Phosphorylation	RSRSSSSTPTSPKPL CCCCCCCCCCCCCCC		30576142
1253	Phosphorylation	RSSSSTPTSPKPLLQ CCCCCCCCCCCCCCC		27794612
1254	Phosphorylation	SSSSTPTSPKPLLQS CCCCCCCCCCCCCCC		25159151
1255	Phosphorylation	SSSTPTSPKPLLQSP CCCCCCCCCCCCCCC		33259812
1261	Phosphorylation	SPKPLLQSPKPSLAA CCCCCCCCCCCCCCC		29255136
1265	Phosphorylation	LLQSPKPSLAARPVI CCCCCCCCCCCCCCC		30576142
1278	Phosphorylation	VIPQKPRTASRPDDI CCCCCCCCCCCCCCC		23927012
1280	Phosphorylation	PQKPRTASRPDDIPD CCCCCCCCCCCCCCC		28355574
1288	Phosphorylation	RPDDIPDSPSSPKVA CCCCCCCCCCCCCEE		25159151
1290	Phosphorylation	DDIPDSPSSPKVALL CCCCCCCCCCCEECC		25159151
1291	Phosphorylation	DIPDSPSSPKVALLP CCCCCCCCCCEECCC		25159151
1306	Phosphorylation	PVLKKVPSDKERDGQ HHHHCCCCCCCCCCC		26657352
1314	Phosphorylation	DKERDGQSSPQPSPR CCCCCCCCCCCCCCC		30266825
1315	Phosphorylation	KERDGQSSPQPSPRT CCCCCCCCCCCCCCC		23401153
1319	Phosphorylation	GQSSPQPSPRTFSQE CCCCCCCCCCCCCHH		30266825
1322	Phosphorylation	SPQPSPRTFSQEVSR CCCCCCCCCCHHHHH		30257219
1324	Phosphorylation	QPSPRTFSQEVSRRS CCCCCCCCHHHHHCH		25159151
1328	Phosphorylation	RTFSQEVSRRSWGQQ CCCCHHHHHCHHHHH		30257219
1331	Phosphorylation	SQEVSRRSWGQQAQE CHHHHHCHHHHHHHH		19664994
1337	Ubiquitination	RSWGQQAQEYQEQKQ CHHHHHHHHHHHHHH		29967540
1339	Phosphorylation	WGQQAQEYQEQKQRS HHHHHHHHHHHHHHH		30266825
1343	Ubiquitination	AQEYQEQKQRSSSKD HHHHHHHHHHHCCCC		29967540
1346	Phosphorylation	YQEQKQRSSSKDGHQ HHHHHHHHCCCCCCC		29978859
1347	Phosphorylation	QEQKQRSSSKDGHQG HHHHHHHCCCCCCCC		29978859
1348	Phosphorylation	EQKQRSSSKDGHQGS HHHHHHCCCCCCCCC		26657352
1355	Phosphorylation	SKDGHQGSKSNDSGE CCCCCCCCCCCCCCH		29978859
1357	Phosphorylation	DGHQGSKSNDSGEEA CCCCCCCCCCCCHHH		30266825
1360	Phosphorylation	QGSKSNDSGEEAEKE CCCCCCCCCHHHHHH		23927012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CARL1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CARL1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CARL1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SLAI2_HUMAN	SLAIN2	physical	22939629
RPP30_HUMAN	RPP30	physical	22939629
CAPZB_HUMAN	CAPZB	physical	26344197
HSF1_HUMAN	HSF1	physical	27173435

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.