dbPTM

BRSK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BRSK1_MOUSE
UniProt AC	Q5RJI5
Protein Name	Serine/threonine-protein kinase BRSK1
Gene Name	Brsk1
Organism	Mus musculus (Mouse).
Sequence Length	778
Subcellular Localization	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, synapse. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage. Localizes to synaptic vesi
Protein Description	Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C..
Protein Sequence	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLLATNGTPLP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	SKEGGGGSPAYHLPH CCCCCCCCCCCCCCC	15.05	22817900
42	Ubiquitination	RLEKTLGKGQTGLVK EEEEECCCCCCCEEE	51.46	22790023
167	Ubiquitination	ENLLLDEKNNIRIAD HHCCCCCCCCCCCCH	55.77	22790023
189	Phosphorylation	VGDSLLETSCGSPHY CCCHHHHCCCCCCCC	29.32	22322096
190	Phosphorylation	GDSLLETSCGSPHYA CCHHHHCCCCCCCCC	13.70	22322096
193	Phosphorylation	LLETSCGSPHYACPE HHHCCCCCCCCCCCH	16.90	-
275	Phosphorylation	VEPEKRLSLEQIQKH CCHHHCCCHHHHHHC	33.61	22817900
308	Phosphorylation	GRRVAMRSLPSNGEL CCCEEEEECCCCCCC	31.39	22817900
311	Phosphorylation	VAMRSLPSNGELDPD EEEEECCCCCCCCHH	63.86	22817900
384	Phosphorylation	PPRKRVDSPMLSRHG CCCCCCCCHHHHHCC	14.89	25521595
388	Phosphorylation	RVDSPMLSRHGKRRP CCCCHHHHHCCCCCC	18.91	22871156
399	Phosphorylation	KRRPERKSMEVLSIT CCCCCCCCEEEEEEC	26.79	25521595
404	Phosphorylation	RKSMEVLSITDAGSG CCCEEEEEECCCCCC	28.72	22807455
406	Phosphorylation	SMEVLSITDAGSGGS CEEEEEECCCCCCCC	19.20	24759943
410	Phosphorylation	LSITDAGSGGSPVPT EEECCCCCCCCCCCH	41.52	28066266
413	Phosphorylation	TDAGSGGSPVPTRRA CCCCCCCCCCCHHHH	26.67	23527152
417	Phosphorylation	SGGSPVPTRRALEMA CCCCCCCHHHHHHHH	32.78	19060867
427	Phosphorylation	ALEMAQHSQRSRSVS HHHHHHHHHHHHCCC	18.40	22324799
430	Phosphorylation	MAQHSQRSRSVSGAS HHHHHHHHHCCCCCC	22.67	19060867
432	Phosphorylation	QHSQRSRSVSGASTG HHHHHHHCCCCCCCC	23.14	25521595
434	Phosphorylation	SQRSRSVSGASTGLS HHHHHCCCCCCCCCC	29.86	19060867
437	Phosphorylation	SRSVSGASTGLSSSP HHCCCCCCCCCCCCC	26.91	30372032
438	Phosphorylation	RSVSGASTGLSSSPL HCCCCCCCCCCCCCC	41.42	25293948
441	Phosphorylation	SGASTGLSSSPLSSP CCCCCCCCCCCCCCC	30.07	18388127
442	Phosphorylation	GASTGLSSSPLSSPR CCCCCCCCCCCCCCC	40.80	18388127
443	Phosphorylation	ASTGLSSSPLSSPRS CCCCCCCCCCCCCCC	26.87	25521595
446	Phosphorylation	GLSSSPLSSPRSPVF CCCCCCCCCCCCCCE	41.50	19060867
447	Phosphorylation	LSSSPLSSPRSPVFS CCCCCCCCCCCCCEE	31.83	25521595
450	Phosphorylation	SPLSSPRSPVFSFSP CCCCCCCCCCEEECC	28.88	21082442
454	Phosphorylation	SPRSPVFSFSPEPGA CCCCCCEEECCCCCC	25.67	22322096
456	Phosphorylation	RSPVFSFSPEPGAGD CCCCEEECCCCCCCC	27.44	25521595
466	Methylation	PGAGDEARGGGSPTS CCCCCCCCCCCCCCC	42.09	24129315
472	Phosphorylation	ARGGGSPTSKTQTLP CCCCCCCCCCCCCCC	44.55	20139300
473	Phosphorylation	RGGGSPTSKTQTLPS CCCCCCCCCCCCCCC	36.77	20139300
481	Methylation	KTQTLPSRGPRGGGA CCCCCCCCCCCCCCC	58.14	24129315
484	Methylation	TLPSRGPRGGGAGEQ CCCCCCCCCCCCCCC	60.25	24129315
498	Methylation	QPPPPSARSTPLPGP CCCCCCCCCCCCCCC	45.46	24129315
499	Phosphorylation	PPPPSARSTPLPGPP CCCCCCCCCCCCCCC	33.81	25521595
500	Phosphorylation	PPPSARSTPLPGPPG CCCCCCCCCCCCCCC	24.24	24925903
508	Phosphorylation	PLPGPPGSPRSSGGT CCCCCCCCCCCCCCC	23.94	25521595
511	Phosphorylation	GPPGSPRSSGGTPLH CCCCCCCCCCCCCCC	36.40	18388127
512	Phosphorylation	PPGSPRSSGGTPLHS CCCCCCCCCCCCCCC	42.39	18388127
515	Phosphorylation	SPRSSGGTPLHSPLH CCCCCCCCCCCCCCC	26.63	22322096
519	Phosphorylation	SGGTPLHSPLHTPRA CCCCCCCCCCCCCCC	36.52	18388127
523	Phosphorylation	PLHSPLHTPRASPTG CCCCCCCCCCCCCCC	23.35	22324799
525	Methylation	HSPLHTPRASPTGTP CCCCCCCCCCCCCCC	49.18	24129315
527	Phosphorylation	PLHTPRASPTGTPGT CCCCCCCCCCCCCCC	25.03	18388127
529	Phosphorylation	HTPRASPTGTPGTTP CCCCCCCCCCCCCCC	51.71	18388127
531	Phosphorylation	PRASPTGTPGTTPPP CCCCCCCCCCCCCCC	22.48	18388127
534	Phosphorylation	SPTGTPGTTPPPSPG CCCCCCCCCCCCCCC	37.01	18388127
535	Phosphorylation	PTGTPGTTPPPSPGG CCCCCCCCCCCCCCC	39.45	18388127
539	Phosphorylation	PGTTPPPSPGGGVGG CCCCCCCCCCCCCCH	41.78	18388127
550	Methylation	GVGGAAWRSRLNSIR CCCHHHHHHHHHHHH	13.74	24129315
551	Phosphorylation	VGGAAWRSRLNSIRN CCHHHHHHHHHHHHH	30.34	22817900
555	Phosphorylation	AWRSRLNSIRNSFLG HHHHHHHHHHHHHCC	27.76	22817900
559	Phosphorylation	RLNSIRNSFLGSPRF HHHHHHHHHCCCHHH	16.40	22817900
563	Phosphorylation	IRNSFLGSPRFHRRK HHHHHCCCHHHHCCC	18.30	25521595
575	Phosphorylation	RRKMQVPTAEEMSSL CCCCCCCCHHHHHHC	48.09	24925903
580	Phosphorylation	VPTAEEMSSLTPESS CCCHHHHHHCCCCCC	26.02	25293948
581	Phosphorylation	PTAEEMSSLTPESSP CCHHHHHHCCCCCCH	34.65	24925903
583	Phosphorylation	AEEMSSLTPESSPEL HHHHHHCCCCCCHHH	27.27	24925903
586	Phosphorylation	MSSLTPESSPELAKR HHHCCCCCCHHHHHH	53.01	25521595
587	Phosphorylation	SSLTPESSPELAKRS HHCCCCCCHHHHHHC	22.26	25521595
594	Phosphorylation	SPELAKRSWFGNFIS CHHHHHHCCCCCEEC	26.50	29899451
601	Phosphorylation	SWFGNFISLDKEEQI CCCCCEECCCHHHEE	26.78	29899451
669	Phosphorylation	RFQVDISSSEGPEPS EEEEECCCCCCCCCC	32.51	28059163
676	Phosphorylation	SSEGPEPSPRRDGSS CCCCCCCCCCCCCCC	29.40	22817900
683	Phosphorylation	SPRRDGSSGGGIYSV CCCCCCCCCCCEEEE	47.16	21183079
688	Phosphorylation	GSSGGGIYSVTFTLI CCCCCCEEEEEEEEE	10.60	21183079
689	Phosphorylation	SSGGGIYSVTFTLIS CCCCCEEEEEEEEEE	16.86	21183079
691	Phosphorylation	GGGIYSVTFTLISGP CCCEEEEEEEEEECC	12.64	28576409
693	Phosphorylation	GIYSVTFTLISGPSR CEEEEEEEEEECCCH	17.51	21183079
739	Phosphorylation	AQTRPAGTPPRSLQP CCCCCCCCCCCCCCC	32.02	-
751	Phosphorylation	LQPPPGRSDPDLSSS CCCCCCCCCCCCCCC	60.45	23527152
756	Phosphorylation	GRSDPDLSSSPRRGP CCCCCCCCCCCCCCC	35.83	23527152
757	Phosphorylation	RSDPDLSSSPRRGPP CCCCCCCCCCCCCCC	52.22	23527152
758	Phosphorylation	SDPDLSSSPRRGPPK CCCCCCCCCCCCCCC	21.03	23527152

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
189	T	Phosphorylation	Kinase	STK11	Q9WTK7	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
189	T	Phosphorylation	17482548
May be inactivated via dephosphorylation of Thr-189 by PP2C.
189	T	Phosphorylation	17482548
Not phosphorylated at Thr-189 by CaMKK2.
189	T	Phosphorylation	17482548
Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BRSK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of BRSK1_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BRSK1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"LKB1 and SAD kinases define a pathway required for the polarizationof cortical neurons."; Barnes A.P., Lilley B.N., Pan Y.A., Plummer L.J., Powell A.W.,Raines A.N., Sanes J.R., Polleux F.; Cell 129:549-563(2007). Cited for: FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT THR-189, ANDMUTAGENESIS OF THR-189.	17482548 [Abstract]

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