BRK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BRK1_HUMAN
• UniProt AC: Q8WUW1
• Protein Name: Protein BRICK1
• Gene Name: BRK1
• Organism: Homo sapiens (Human).
• Sequence Length: 75
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity)..
• Protein Sequence: MAGQEDPVQREIHQDWANREYIEIITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTKGETLT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAGQEDPVQ ------CCCCCCHHH	28.51	22814378
21	Phosphorylation	QDWANREYIEIITSS HHHHHHHHHHHHHHH	10.70	30243723
21 (in isoform 2)	Phosphorylation	-	10.70	27642862
26	Phosphorylation	REYIEIITSSIKKIA HHHHHHHHHHHHHHH	23.29	30243723
27	Phosphorylation	EYIEIITSSIKKIAD HHHHHHHHHHHHHHH	21.47	30243723
28	Phosphorylation	YIEIITSSIKKIADF HHHHHHHHHHHHHHH	29.76	30243723
30	Ubiquitination	EIITSSIKKIADFLN HHHHHHHHHHHHHHH	39.82	21890473
30 (in isoform 1)	Ubiquitination	-	39.82	21890473
30	Ubiquitination	EIITSSIKKIADFLN HHHHHHHHHHHHHHH	39.82	21890473
30 (in isoform 2)	Ubiquitination	-	39.82	21890473
31 (in isoform 2)	Ubiquitination	-	41.26	-
31	Ubiquitination	IITSSIKKIADFLNS HHHHHHHHHHHHHHH	41.26	23000965
41	Sulfoxidation	DFLNSFDMSCRSRLA HHHHHCCHHHHHHHH	3.72	21406390
46	Methylation	FDMSCRSRLATLNEK CCHHHHHHHHHHHHH	14.69	-
53 (in isoform 2)	Ubiquitination	-	50.49	21890473
53	Ubiquitination	RLATLNEKLTALERR HHHHHHHHHHHHHHH	50.49	23000965
53	2-Hydroxyisobutyrylation	RLATLNEKLTALERR HHHHHHHHHHHHHHH	50.49	-
53	Ubiquitination	RLATLNEKLTALERR HHHHHHHHHHHHHHH	50.49	21890473
53 (in isoform 1)	Ubiquitination	-	50.49	21890473
63 (in isoform 2)	Phosphorylation	-	11.16	27642862
63	Phosphorylation	ALERRIEYIEARVTK HHHHHHHHHHHHHCC	11.16	25884760
70	Ubiquitination	YIEARVTKGETLT-- HHHHHHCCCCCCC--	52.47	33845483
70 (in isoform 2)	Ubiquitination	-	52.47	-
70 (in isoform 1)	Ubiquitination	-	52.47	21890473
75	Phosphorylation	VTKGETLT------- HCCCCCCC-------	43.94	25332170

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BRK1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BRK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BRK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DTBP1_HUMAN	DTNBP1	physical	25416956

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
PubMed: 19413330

Phosphorylation

"An extensive survey of tyrosine phosphorylation revealing new sitesin human mammary epithelial cells.";
Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A.,Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D.,Wiley H.S., Qian W.-J.;
J. Proteome Res. 8:3852-3861(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-63, AND MASSSPECTROMETRY.
PubMed: 19534553