BKI1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BKI1_ARATH
• UniProt AC: Q9FMZ0
• Protein Name: BRI1 kinase inhibitor 1
• Gene Name: BKI1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 337
• Subcellular Localization: Cell membrane . Cytoplasm . Once phosphorylated following brassinosteroid treatment, displaced from the plasma membrane into the cytosol where it is inactive.
• Protein Description: Negative regulator of brassinosteroid signaling. When associated to the membrane, limits the interaction of BRI1 with BAK1 by binding to the kinase-inactive form of BRI1..
• Protein Sequence: METNLQQVKNSSQTFSEKQNPKQEASPSPISSTCSSPSHDFSFTISLQPLSSSSKHISPTLRSPSKTTSSYQQTDPFAVDLSPADEIFFHGHLLPLHLLSHLPVSPRTSTGSYNDGFTLPVKDILPDQPTNNNNNTENAITNISTEAKDDNTEDKAEGEIRVKTKPIKSFSLFGLSKWRKGFESNEREQEQQQQKIKKPMSLDLSHAVKKYIRMLFQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNSKTMINGSYNKRDLIRGRRGELFSAPASMRTSPTNSGHLRVSTAGLSSSSGSTSSSSSDSTMEELQAAIQAAIAHCKNSSAVDRDDKVKDS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
58	Phosphorylation	SSSSKHISPTLRSPS CCCCCCCCCCCCCCC	16.14	25561503
60	Phosphorylation	SSKHISPTLRSPSKT CCCCCCCCCCCCCCC	28.19	25561503
63	Phosphorylation	HISPTLRSPSKTTSS CCCCCCCCCCCCCCC	36.32	25561503
105	Phosphorylation	LLSHLPVSPRTSTGS HHHCCCCCCCCCCCC	13.51	29654922
108	Phosphorylation	HLPVSPRTSTGSYND CCCCCCCCCCCCCCC	33.74	25561503
109	Phosphorylation	LPVSPRTSTGSYNDG CCCCCCCCCCCCCCC	31.86	30407730
110	Phosphorylation	PVSPRTSTGSYNDGF CCCCCCCCCCCCCCE	29.54	30407730
112	Phosphorylation	SPRTSTGSYNDGFTL CCCCCCCCCCCCEEE	22.35	30407730
113	Phosphorylation	PRTSTGSYNDGFTLP CCCCCCCCCCCEEEE	20.72	30407730
118	Phosphorylation	GSYNDGFTLPVKDIL CCCCCCEEEEHHHHC	35.36	25561503
171	Phosphorylation	TKPIKSFSLFGLSKW CCCCCEEEECCHHHH	29.85	25561503
211	Phosphorylation	LSHAVKKYIRMLFQK HHHHHHHHHHHHHHH	6.50	21289069
270	Phosphorylation	GRRGELFSAPASMRT CCCCCCEECCCHHCC	46.06	29654922
274	Phosphorylation	ELFSAPASMRTSPTN CCEECCCHHCCCCCC	14.03	29654922
277	Phosphorylation	SAPASMRTSPTNSGH ECCCHHCCCCCCCCC	30.34	25561503
278	Phosphorylation	APASMRTSPTNSGHL CCCHHCCCCCCCCCE	21.25	29654922
280	Phosphorylation	ASMRTSPTNSGHLRV CHHCCCCCCCCCEEE	41.61	29654922
282	Phosphorylation	MRTSPTNSGHLRVST HCCCCCCCCCEEEEE	30.02	25561503

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BKI1_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BKI1_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BKI1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BRI1_ARATH	BRI1	physical	16857903

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Tyrosine phosphorylation controls brassinosteroid receptor activationby triggering membrane release of its kinase inhibitor.";
Jaillais Y., Hothorn M., Belkhadir Y., Dabi T., Nimchuk Z.L.,Meyerowitz E.M., Chory J.;
Genes Dev. 25:232-237(2011).
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-211, AND MUTAGENESIS OF179-ARG-LYS-180; 197-LYS-LYS-198; 209-LYS-LYS-210; TYR-211;218-LYS-LYS-219 AND CYS-322.
PubMed: 21289069