BIG1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BIG1_RAT
• UniProt AC: D4A631
• Protein Name: Brefeldin A-inhibited guanine nucleotide-exchange protein 1
• Gene Name: Arfgef1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1846
• Subcellular Localization: Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus . Golgi apparatus, trans-Golgi network . Nucleus. Nucleus, nucleolus. Nucleus matrix. Membrane. Translocates from cytoplasm to membranes and nucleus upon cAMP treatment (By similarity). PRKAR1
• Protein Description: Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity)..
• Protein Sequence: MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGSAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQHHLLQSPVSHHEPESPHLRYLPPQTVDHIAQEQEGDLDPQTHDVDKSLQDDIEPENGSDISSAENEQTEADQATAAETLSKDDVLCDGECEEKPQDIVQSIVEEMVDIIVGDMGEGTAVSASADGNAGAVEDGSDSENVQANGIPGTPISAAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQAGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTSGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCVINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPTSGEAAPPSPSAMSEKQLDAISQKSVDIHDSAQPRSSDNRQQAPLVSVSPASEEVSKGRPTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGIVFQISQPPEQELGINKQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
23	Acetylation	LEKILADKEVKKAHH HHHHHCCHHHHHHHH		22902405
48	Phosphorylation	LEEIKVETEKQSPPH HHHHCCCCCCCCCCC		28432305
52	Phosphorylation	KVETEKQSPPHGEAK CCCCCCCCCCCCCCC		30411139
70	Phosphorylation	GTLPPVKSKTNFIEA CCCCCCCCCCCCEEC		25575281
109	Phosphorylation	CLQKLIAYGHLTGSA HHHHHHHHCHHHCCC		25575281
113	Phosphorylation	LIAYGHLTGSAPDST HHHHCHHHCCCCCCC		25575281
115	Phosphorylation	AYGHLTGSAPDSTTP HHCHHHCCCCCCCCC		25575281
119	Phosphorylation	LTGSAPDSTTPGKKL HHCCCCCCCCCCHHH		25575281
120	Phosphorylation	TGSAPDSTTPGKKLI HCCCCCCCCCCHHHH		25575281
121	Phosphorylation	GSAPDSTTPGKKLID CCCCCCCCCCHHHHH		25575281
234	Phosphorylation	QQHHLLQSPVSHHEP HHHHHHCCCCCCCCC		28432305
237	Phosphorylation	HLLQSPVSHHEPESP HHHCCCCCCCCCCCC		28432305
243	Phosphorylation	VSHHEPESPHLRYLP CCCCCCCCCCCCCCC		28432305
269	Phosphorylation	EGDLDPQTHDVDKSL CCCCCCCCCCCCHHH		-
286	Phosphorylation	DIEPENGSDISSAEN CCCCCCCCCCCHHHC		22673903
289	Phosphorylation	PENGSDISSAENEQT CCCCCCCCHHHCHHC		22673903
290	Phosphorylation	ENGSDISSAENEQTE CCCCCCCHHHCHHCH		22673903
296	Phosphorylation	SSAENEQTEADQATA CHHHCHHCHHHHHHH		28689409
391	Phosphorylation	SLPDDRLSVSSNDTQ CCCCCCEECCCCCCC		27097102
393	Phosphorylation	PDDRLSVSSNDTQES CCCCEECCCCCCCCC		21738781
394	Phosphorylation	DDRLSVSSNDTQESG CCCEECCCCCCCCCC		27097102
397	Phosphorylation	LSVSSNDTQESGNSS EECCCCCCCCCCCCC		27097102
400	Phosphorylation	SSNDTQESGNSSGPS CCCCCCCCCCCCCCC		27097102
403	Phosphorylation	DTQESGNSSGPSPGA CCCCCCCCCCCCCCC		27097102
404	Phosphorylation	TQESGNSSGPSPGAK CCCCCCCCCCCCCCC		27097102
407	Phosphorylation	SGNSSGPSPGAKFSH CCCCCCCCCCCCHHH		28689409
659	Phosphorylation	HPETINRYGSLNSLE CHHHHHCCCCCCCCC		22673903
661	Phosphorylation	ETINRYGSLNSLEST HHHHCCCCCCCCCCC		22673903
664	Phosphorylation	NRYGSLNSLESTSSS HCCCCCCCCCCCCCC		22673903
667	Phosphorylation	GSLNSLESTSSSGIG CCCCCCCCCCCCCCC		22673903
668	Phosphorylation	SLNSLESTSSSGIGS CCCCCCCCCCCCCCC		22673903
669	Phosphorylation	LNSLESTSSSGIGSY CCCCCCCCCCCCCCC		22673903
670	Phosphorylation	NSLESTSSSGIGSYS CCCCCCCCCCCCCCC		22673903
671	Phosphorylation	SLESTSSSGIGSYST CCCCCCCCCCCCCCC		22673903
675	Phosphorylation	TSSSGIGSYSTQMSG CCCCCCCCCCCCCCC		22673903
676	Phosphorylation	SSSGIGSYSTQMSGT CCCCCCCCCCCCCCC		22673903
677	Phosphorylation	SSGIGSYSTQMSGTD CCCCCCCCCCCCCCC		22673903
678	Phosphorylation	SGIGSYSTQMSGTDN CCCCCCCCCCCCCCC		22673903
681	Phosphorylation	GSYSTQMSGTDNPEQ CCCCCCCCCCCCHHH		22673903
683	Phosphorylation	YSTQMSGTDNPEQFE CCCCCCCCCCHHHHH		22673903
754	Ubiquitination	EFLGDNDKFNKEVMY HHCCCCCCCCCHHHH		-
1076	Phosphorylation	TVRGREGSLTGTKDQ EECCCCCCCCCCCCC		29779826
1078	Phosphorylation	RGREGSLTGTKDQAP CCCCCCCCCCCCCCC		22673903
1080	Phosphorylation	REGSLTGTKDQAPDE CCCCCCCCCCCCCCC		25575281
1542	Phosphorylation	ALLTWRPTSGEAAPP HHCCCCCCCCCCCCC		28689409
1550	Phosphorylation	SGEAAPPSPSAMSEK CCCCCCCCCCCCCHH		27097102
1552	Phosphorylation	EAAPPSPSAMSEKQL CCCCCCCCCCCHHHH		28432305
1555	Phosphorylation	PPSPSAMSEKQLDAI CCCCCCCCHHHHHHH		27097102
1563	Phosphorylation	EKQLDAISQKSVDIH HHHHHHHHHCCCCCC		29779826
1566	Phosphorylation	LDAISQKSVDIHDSA HHHHHHCCCCCCCCC		29779826
1572	Phosphorylation	KSVDIHDSAQPRSSD CCCCCCCCCCCCCCC		28689409
1588	Phosphorylation	RQQAPLVSVSPASEE CCCCCEEEECCCCCC		28432305
1590	Phosphorylation	QAPLVSVSPASEEVS CCCEEEECCCCCCHH		28432305
1593	Phosphorylation	LVSVSPASEEVSKGR EEEECCCCCCHHCCC		30181290

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BIG1_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BIG1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BIG1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of BIG1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.