BECN1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BECN1_RAT
• UniProt AC: Q91XJ1
• Protein Name: Beclin-1
• Gene Name: Becn1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 448
• Subcellular Localization: Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein
• Protein Description: Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense (By similarity).; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors..
• Protein Sequence: MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGESQEEEANSGEEPFIETRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEMLEQMNEGDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQMQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSAPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEGSKASS -------CCCCCCCC	13.69	-
7	Phosphorylation	-MEGSKASSSTMQVS -CCCCCCCCCCCEEE	28.67	27097102
8	Phosphorylation	MEGSKASSSTMQVSF CCCCCCCCCCCEEEE	34.39	27097102
9	Phosphorylation	EGSKASSSTMQVSFV CCCCCCCCCCEEEEE	25.96	27097102
10	Phosphorylation	GSKASSSTMQVSFVC CCCCCCCCCEEEEEE	17.36	27097102
28	Phosphorylation	SQPLKLDTSFKILDR CCCCCCCCCEEEECE	46.64	25575281
29	Phosphorylation	QPLKLDTSFKILDRV CCCCCCCCEEEECEE	24.98	25575281
88	Phosphorylation	IPPARMMSTESANSF CCCHHHCCCCCCCCE	21.49	-
91	Phosphorylation	ARMMSTESANSFTLI HHHCCCCCCCCEEEE	32.60	22276854
94	Phosphorylation	MSTESANSFTLIGEA CCCCCCCCEEEEEEC	21.23	22276854
117	Phosphorylation	LSRRLKVTGDLFDIM HHHHHHHCCCHHHHH	24.32	-
183	Acetylation	EQLQRELKELALEEE HHHHHHHHHHHHHHH	46.02	22902405
232	Phosphorylation	AQYQREYSEFKRQQL HHHHHHHHHHHHHHC	31.63	-
293	Phosphorylation	FRLGRLPSAPVEWNE EECCCCCCCCCCHHH	50.20	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
88	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot
91	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot
94	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot
117	T	Phosphorylation	Kinase	DAPK1	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
11	K	ubiquitylation	Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages (By similarity).	-
117	T	Phosphorylation	Phosphorylation at Thr-117 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.	-
400	K	ubiquitylation	'Lys-48'-linked polyubiquitination of Lys-400 leads to degradation (By similarity).	-
400	K	ubiquitylation	Polyubiquitinated at Lys-400 with 'Lys-48'-linkages (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BECN1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of BECN1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.